LOCUS       pTAL-CMV-T7-v2-018170        5692 bp    DNA     circular     31-OCT-2012
DEFINITION  LMBP 8949.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8949
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
VERSION     pCLS14977.2
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|Private
COMMENT     VNTDATE|641391272|
COMMENT     VNTDBDATE|641391272|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTAL-CMV-T7-v2-018170|
COMMENT     VNTAUTHORNAME|teissieroise|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     polyA_signal    2937..3164
                     /vntifkey="25"
                     /label=BGHpA
     rep_origin      3190..3863
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             complement(4011..4868)
                     /vntifkey="4"
                     /label=AmpR
     promoter        5030..5617
                     /vntifkey="29"
                     /label=pCMV
     promoter        5661..5680
                     /vntifkey="30"
                     /label=T7
     misc_feature    55..2886
                     /vntifkey="21"
                     /label=TALEN_018170
     misc_structure  553..2142
                     /vntifkey="88"
                     /label=DNA\binding\domain
     misc_feature    55..552
                     /vntifkey="21"
                     /label=N-ter\domain
     misc_feature    2143..2886
                     /vntifkey="21"
                     /label=C-ter\domain
BASE COUNT     1364 a      1559 c      1532 g      1237 t 
ORIGIN
        1 gctagcctta ggcgcgccac tagcgctctc gaggtcacgc gccaagccgc caacatgggc 
       61 gatcctaaaa agaaacgtaa ggtcatcgat aaggagaccg ccgctgccaa gttcgagaga 
      121 cagcacatgg acagcatcga tatcgccgat ctacgcacgc tcggctacag ccagcagcaa 
      181 caggagaaga tcaaaccgaa ggttcgttcg acagtggcgc agcaccacga ggcactggtc 
      241 ggccacgggt ttacacacgc gcacatcgtt gcgttaagcc aacacccggc agcgttaggg 
      301 accgtcgctg tcaagtatca ggacatgatc gcagcgttgc cagaggcgac acacgaagcg 
      361 atcgttggcg tcggcaaaca gtggtccggc gcacgcgctc tggaggcctt gctcacggtg 
      421 gcgggagagt tgagaggtcc accgttacag ttggacacag gccaacttct caagattgca 
      481 aaacgtggcg gcgtgaccgc agtggaggca gtgcatgcat ggcgcaatgc actgacgggt 
      541 gccccgctca acttgacccc tcaacaggtc gttgctatcg caagtaacaa tgggggtaaa 
      601 caagctctgg aaactgtaca gcgattgctg cccgtgctct gccaggctca cggcttgact 
      661 cctcagcaag tcgtggccat agcaagcaat ggtggcggaa aacaagccct cgagacagtg 
      721 cagcggcttc ttccagtgct gtgtcaagct catggcctca ctcccgagca ggtcgtagcc 
      781 atcgctagtc atgacggagg taaacaggca cttgaaaccg tccagaggtt gttgccagtg 
      841 ctttgccaag cacacggact cactccacag caagttgtcg ctatcgctag caacgggggt 
      901 ggcaagcagg ctctcgaaac tgtgcagaga ctccttccag tcctgtgcca ggcccacggc 
      961 ctgactcctg aacaagtggt agccatagcc tcccacgatg gtgggaagca ggcactcgag 
     1021 accgttcaga ggctgctgcc tgtcttgtgt caggctcatg ggctgactcc agagcaagtg 
     1081 gttgcgatcg cttctcacga tggaggaaaa caggccctgg aaacggttca gcggttgctg 
     1141 ccagtcctct gccaagcgca cgggctcacc ccagaacagg tcgtcgcaat cgccagtcac 
     1201 gacggcggca agcaagcact ggagacagtt caacgcctgt tgcctgtact ctgtcaggca 
     1261 cacgggctta caccagagca ggtggtcgct attgcgtcca acatcggcgg gaagcaagcg 
     1321 ctcgagactg ttcaggcttt gctcccggta ctctgccaag ctcatggatt gacacctgaa 
     1381 caagttgtag caatcgctag caatatcgga gggaaacagg ccttggagac tgtgcaggcc 
     1441 ctgctgccgg tcctgtgcca agcacatggg ttgacccctc agcaggtagt ggccattgct 
     1501 tcaaacaacg gcggtaaaca ggctttggag acagtccaga ggctgctgcc cgtgctgtgc 
     1561 caggcgcacg gactcactcc agagcaggtg gtcgcgatag caagccatga cggcgggaag 
     1621 caggcactgg agactgtcca gcgcctgctg ccagtgcttt gccaggcaca tggcctgaca 
     1681 ccccagcagg tcgtggctat tgcatcaaat gggggtggga aacaagcact tgaaaccgtg 
     1741 cagaggctcc tgcccgttct ctgtcaagcg cacggcctga ctccagaaca ggtcgtcgct 
     1801 atcgctagtc acgacggggg taagcaagcc cttgaaacag ttcagcgctt gctccctgta 
     1861 ctttgtcagg cccacgggct tactcccgaa caagtcgtag ccattgccag ccacgacggg 
     1921 ggcaaacagg ccctggaaac agtacagcgc ctcctccctg tcctttgcca agcccacgga 
     1981 ctgacacctc aacaagtggt ggccatcgca tccaataatg gaggaaaaca ggctctcgag 
     2041 accgtccaac gactgttgcc agttctgtgt caggcgcatg gtctgacgcc ccagcaagta 
     2101 gttgccatcg cctccaacgg cggcggcaga cccgccctgg agagcattgt tgcccagtta 
     2161 tctcgccctg atccggcgtt ggccgcgttg accaacgacc acctcgtcgc cttggcctgc 
     2221 ctcggcgggc gtcctgcgct ggatgcagtg aaaaagggat tgggggatcc tatcagccgt 
     2281 tcccagctgg tgaagtccga gctggaggag aagaaatccg agttgaggca caagctgaag 
     2341 tacgtgcccc acgagtacat cgagctgatc gagatcgccc ggaacagcac ccaggaccgt 
     2401 atcctggaga tgaaggtgat ggagttcttc atgaaggtgt acggctacag gggcaagcac 
     2461 ctgggcggct ccaggaagcc cgacggcgcc atctacaccg tgggctcccc catcgactac 
     2521 ggcgtgatcg tggacaccaa ggcctactcc ggcggctaca acctgcccat cggccaggcc 
     2581 gacgaaatgc agaggtacgt ggaggagaac cagaccagga acaagcacat caaccccaac 
     2641 gagtggtgga aggtgtaccc ctccagcgtg accgagttca agttcctgtt cgtgtccggc 
     2701 cacttcaagg gcaactacaa ggcccagctg accaggctga accacatcac caactgcaac 
     2761 ggcgccgtgc tgtccgtgga ggagctcctg atcggcggcg agatgatcaa ggccggcacc 
     2821 ctgaccctgg aggaggtgag gaggaagttc aacaacggcg agatcaactt cgcggccgac 
     2881 tgataactcg agcgatcctc tagacgagct cctcgagcct gcagcagctg aagcttcgac 
     2941 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 
     3001 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 
     3061 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 
     3121 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggatatc ttcttaatta 
     3181 agacctagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     3241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     3301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     3361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     3421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     3481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     3541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     3601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     3661 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     3721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     3781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     3841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     3901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     3961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     4021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     4081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     4141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     4201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     4261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     4321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     4381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     4441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     4501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     4561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     4621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     4681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     4741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     4801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     4861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     4921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     4981 ccccgaaaag tgccacctga caaacttggt accataacta gttcggcgcg ttgacattga 
     5041 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     5101 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     5161 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     5221 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     5281 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     5341 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     5401 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     5461 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     5521 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     5581 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact 
     5641 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tg 
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