LOCUS       pTAL-CMV-T7-v2-018176        5674 bp    DNA     circular     31-OCT-2012
DEFINITION  LMBP 8950.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8950
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
VERSION     pCLS14976.2
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|Private
COMMENT     VNTDATE|641391383|
COMMENT     VNTDBDATE|641391383|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTAL-CMV-T7-v2-018176|
COMMENT     VNTAUTHORNAME|teissieroise|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     polyA_signal    2919..3146
                     /vntifkey="25"
                     /label=BGHpA
     rep_origin      3172..3845
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             complement(3993..4850)
                     /vntifkey="4"
                     /label=AmpR
     promoter        5012..5599
                     /vntifkey="29"
                     /label=pCMV
     promoter        5643..5662
                     /vntifkey="30"
                     /label=T7
     misc_feature    55..2868
                     /vntifkey="21"
                     /label=TALEN_018176
     misc_feature    535..2124
                     /vntifkey="21"
                     /label=DNA\binding\domain
     misc_feature    55..534
                     /vntifkey="21"
                     /label=N-ter\domain
     misc_feature    2125..2868
                     /vntifkey="21"
                     /label=C-ter\domain
BASE COUNT     1356 a      1554 c      1522 g      1242 t 
ORIGIN
        1 gctagcctta ggcgcgccac tagcgctctc gaggtcacgc gccaagccgc caacatgggc 
       61 gatcctaaaa agaaacgtaa ggtcatcgat tacccatacg atgttccaga ttacgctatc 
      121 gatatcgccg atctacgcac gctcggctac agccagcagc aacaggagaa gatcaaaccg 
      181 aaggttcgtt cgacagtggc gcagcaccac gaggcactgg tcggccacgg gtttacacac 
      241 gcgcacatcg ttgcgttaag ccaacacccg gcagcgttag ggaccgtcgc tgtcaagtat 
      301 caggacatga tcgcagcgtt gccagaggcg acacacgaag cgatcgttgg cgtcggcaaa 
      361 cagtggtccg gcgcacgcgc tctggaggcc ttgctcacgg tggcgggaga gttgagaggt 
      421 ccaccgttac agttggacac aggccaactt ctcaagattg caaaacgtgg cggcgtgacc 
      481 gcagtggagg cagtgcatgc atggcgcaat gcactgacgg gtgccccgct caacttgacc 
      541 cctcaacagg tcgttgctat cgcaagtaac aatgggggta aacaagctct ggaaactgta 
      601 cagcgattgc tgcccgtgct ctgccaggct cacggcttga ctcctgagca agtcgtggcc 
      661 atagcaagcc acgatggcgg aaaacaagcc ctcgagacag tgcagcggct tcttccagtg 
      721 ctgtgtcaag ctcatggcct cactcccgag caggtcgtag ccatcgctag tcatgacgga 
      781 ggtaaacagg cacttgaaac cgtccagagg ttgttgccag tgctttgcca agcacacgga 
      841 ctcactccac agcaagttgt cgctatcgct agcaacgggg gtggcaagca ggctctcgaa 
      901 actgtgcaga gactccttcc agtcctgtgc caggcccacg gcctgactcc tgaacaagtg 
      961 gtagccatag cctccaacat cggtgggaag caggcactcg agaccgttca ggctctgctg 
     1021 cctgtcttgt gtcaggctca tgggctgact ccagagcaag tggttgcgat cgcttctcac 
     1081 gacggaggaa aacaggccct ggaaacggtt caaaggttgc tgccagtcct ctgccaagcg 
     1141 cacgggctca ccccacagca ggtcgtcgca atcgccagta acaatggcgg caagcaagca 
     1201 ctggagacag ttcaacgcct gttgcctgta ctctgtcagg cacacgggct tacaccagag 
     1261 caggtggtcg ctattgcgtc ccacgatggc gggaagcaag cgctcgagac tgttcagcgg 
     1321 ttgctcccgg tactctgcca agctcatgga ttgacacctc agcaagttgt agcaatcgct 
     1381 agcaatggtg gagggaaaca ggccttggag actgtgcagc ggctcctgcc ggtcctgtgc 
     1441 caagcacatg ggttgacccc tcagcaggta gtggccattg cttcaaacaa cggcggtaaa 
     1501 caggctttgg agacagtcca gaggctgctg cccgtgctgt gccaggcgca cggactcact 
     1561 ccagagcagg tggtcgcgat agcaagccat gacggcggga agcaggcact ggagactgtc 
     1621 cagcgcctgc tgccagtgct ttgccaggca catggcctga cacccgagca ggtcgtggct 
     1681 attgcatcac atgatggtgg gaaacaagca cttgaaaccg tgcagaggct cctgcccgtt 
     1741 ctctgtcaag cgcacggcct gactccagaa caggtcgtcg ctatcgctag tcacgacggg 
     1801 ggtaagcaag cccttgaaac agttcagcgc ttgctccctg tactttgtca ggcccacggg 
     1861 cttactcccg aacaagtcgt agccattgcc agcaacatcg ggggcaaaca ggccctggaa 
     1921 acagtacagg ctctcctccc tgtcctttgc caagcccacg gactgacacc tgaacaagtg 
     1981 gtggccatcg catcccacga tggaggaaaa caggctctcg agaccgtcca acgactgttg 
     2041 ccagttctgt gtcaggcgca tggtctgacg ccccagcaag tagttgccat cgcctccaac 
     2101 ggcggcggca gacccgccct ggagagcatt gttgcccagt tatctcgccc tgatccggcg 
     2161 ttggccgcgt tgaccaacga ccacctcgtc gccttggcct gcctcggcgg gcgtcctgcg 
     2221 ctggatgcag tgaaaaaggg attgggggat cctatcagcc gttcccagct ggtgaagtcc 
     2281 gagctggagg agaagaaatc cgagttgagg cacaagctga agtacgtgcc ccacgagtac 
     2341 atcgagctga tcgagatcgc ccggaacagc acccaggacc gtatcctgga gatgaaggtg 
     2401 atggagttct tcatgaaggt gtacggctac aggggcaagc acctgggcgg ctccaggaag 
     2461 cccgacggcg ccatctacac cgtgggctcc cccatcgact acggcgtgat cgtggacacc 
     2521 aaggcctact ccggcggcta caacctgccc atcggccagg ccgacgaaat gcagaggtac 
     2581 gtggaggaga accagaccag gaacaagcac atcaacccca acgagtggtg gaaggtgtac 
     2641 ccctccagcg tgaccgagtt caagttcctg ttcgtgtccg gccacttcaa gggcaactac 
     2701 aaggcccagc tgaccaggct gaaccacatc accaactgca acggcgccgt gctgtccgtg 
     2761 gaggagctcc tgatcggcgg cgagatgatc aaggccggca ccctgaccct ggaggaggtg 
     2821 aggaggaagt tcaacaacgg cgagatcaac ttcgcggccg actgataact cgagcgatcc 
     2881 tctagacgag ctcctcgagc ctgcagcagc tgaagcttcg actgtgcctt ctagttgcca 
     2941 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 
     3001 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 
     3061 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 
     3121 tgctggggat gcggtgggct ctatgggata tcttcttaat taagacctag catgtgagca 
     3181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     3241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     3301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     3361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     3421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     3481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     3541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     3601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     3661 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     3721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     3781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     3841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     3901 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     3961 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     4021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     4081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     4141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     4201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     4261 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     4321 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     4381 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     4441 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     4501 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     4561 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     4621 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     4681 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     4741 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     4801 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     4861 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     4981 gacaaacttg gtaccataac tagttcggcg cgttgacatt gattattgac tagttattaa 
     5041 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 
     5101 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 
     5161 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 
     5221 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 
     5281 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 
     5341 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 
     5401 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 
     5461 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 
     5521 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 
     5581 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa 
     5641 attaatacga ctcactatag ggagacccaa gctg 
//
