LOCUS       pENTR-L4R1-hCMV         3229 bp    DNA     circular      3-FEB-2014
DEFINITION  LMBP 8997.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8997
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 3-FEB-2014
COMMENT     VNTDATE|688737899|
COMMENT     VNTNAME|pENTR-L4R1-hCMV|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             1617..2432
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      2949..122
                     /vntifkey="33"
                     /label=pMB1\ori
     attenuator      593..688
                     /vntifkey="0"
                     /label=attL4
     promoter        691..1274
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        complement(1473..1489)
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      427..470
                     /vntifkey="43"
                     /label=T1\terminator
     attenuator      complement(1275..1399)
                     /vntifkey="0"
                     /label=attR1
     terminator      complement(268..295)
                     /vntifkey="43"
                     /label=T2\terminator
BASE COUNT      837 a       759 c       781 g       852 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 
      661 ataatgccaa ctttgtatag aaaagttgtt gacattgatt attgactagt tattaatagt 
      721 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 
      781 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 
      841 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 
      901 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 
      961 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 
     1021 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt 
     1081 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 
     1141 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 
     1201 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 
     1261 atataagcag agctccaagt ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa 
     1321 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac 
     1381 aacatatgca gtcactatga accaactact tagatggtat tagtgacctg tagaatcgag 
     1441 ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag 
     1501 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 
     1561 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 
     1621 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 
     1681 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 
     1741 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 
     1801 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 
     1861 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 
     1921 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 
     1981 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 
     2041 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 
     2101 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 
     2161 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 
     2221 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 
     2281 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 
     2341 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 
     2401 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 
     2461 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg 
     2521 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     2581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     2641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     2701 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     2761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     2821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     2881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     2941 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga 
     3001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     3061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     3121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     3181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt 
//
