ID   pENTR-L4R1-hCMV standard; circular DNA;    ; 3229 BP.
DE   LMBP 8997.
CC   Accession number: LMBP 8997
CC   (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium)
CC   Last modified: 3-FEB-2014
CC
CC   VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
CC   VNTAUTHORTEL|0032-9-33-13-843|
CC   VNTAUTHOREML|bccm.genecorner@UGent.be|
CC   VNTAUTHORWWW|www.genecorner.ugent.be|
CC   VNTAUTHORAD1|Ghent University|
CC   VNTAUTHORAD2|Technologiepark 927|
CC   VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
CC
CC   VNTREPLTYPE|Plasmid
FH   Key             Location/Qualifiers
FH
FT   CDS             1617..2432
FT                   /vntifkey="4"
FT                   /label=Tn903\neomycin\resistance
FT   rep_origin      2949..122
FT                   /vntifkey="33"
FT                   /label=pMB1\ori
FT   attenuator      593..688
FT                   /vntifkey="0"
FT                   /label=attL4
FT   promoter        691..1274
FT                   /vntifkey="29"
FT                   /label=hCMV-IE\promoter\and\enhancer
FT   promoter        complement(1473..1489)
FT                   /vntifkey="30"
FT                   /label=T7\promoter
FT   terminator      427..470
FT                   /vntifkey="43"
FT                   /label=T1\terminator
FT   attenuator      complement(1275..1399)
FT                   /vntifkey="0"
FT                   /label=attR1
FT   terminator      complement(268..295)
FT                   /vntifkey="43"
FT                   /label=T2\terminator
SQ   Sequence 3229 BP; 837 A; 758 C; 782 G; 852 t;
     ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga        60
     taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga       120
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca       180
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc       240
     tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta       300
     gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc       360
     acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa       420
     caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg       480
     gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa       540
     aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg       600
     attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt       660
     ataatgccaa ctttgtatag aaaagttgtt gacattgatt attgactagt tattaatagt       720
     aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta       780
     cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga       840
     cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt       900
     tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta       960
     ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg      1020
     actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt      1080
     tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc      1140
     accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat      1200
     gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct      1260
     atataagcag agctccaagt ttgtacaaaa aagttgaacg agaaacgtaa aatgatataa      1320
     atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac      1380
     aacatatgca gtcactatga accaactact tagatggtat tagtgacctg tagaatcgag      1440
     ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag      1500
     ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata      1560
     aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg      1620
     ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg      1680
     atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc      1740
     gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg      1800
     ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc      1860
     cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc      1920
     ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg      1980
     atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta      2040
     acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg      2100
     atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa      2160
     tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg      2220
     ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa      2280
     tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt      2340
     cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc      2400
     agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact      2460
     ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg      2520
     tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt      2580
     gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag      2640
     cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca      2700
     gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca      2760
     agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg      2820
     ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg      2880
     cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct      2940
     acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga      3000
     gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc      3060
     ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg      3120
     agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg      3180
     cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt                  3229
//