LOCUS       pPCV                    3428 bp    DNA     circular     20-FEB-2014
DEFINITION  LMBP 9004.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9004
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-FEB-2014
COMMENT     VNTDATE|688730399|
COMMENT     VNTNAME|pPCV|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPCV" 0 3428 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 662 0 1219 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "PvuII" "cagctg" 4 0 2 530 0 1445 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 658 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XcmI" "ccannnnnnnnntgg" 9 0 2 675 0 1208 0 0 
COMMENT            "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #5=(CFSignal (CObList) "lacZ fragment" 4 0 1 460 661 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(460..816)") 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 0 626 642 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "626..642") 
COMMENT        #7=(CFSignal (CObList) "T3 promoter" 30 0 1 1242 1258 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(775..791)") 
COMMENT        #8=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 21 0 1 1284 1321 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(817..854)") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 1322 1405 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(855..938)") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1497 1899 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1030..1432)") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2440 3300 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1973..2833)") 
COMMENT        #12=(CFSignal (CObList) "URA3 fragment" 4 0 0 678 1199 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "Last modified: 20-FEB-2014" 2 0 0 0 0 0 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 02/03/14 09:59" 1) 
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COMMENT                          "Last Modified: 02/20/14 04:49" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3428 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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COMMENT                                        "lacZ fragment coding for the \346 peptide" 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(460..661)
                     /vntifkey="4"
                     /label=lacZ\fragment
                     /note="lacZ fragment coding for the æ peptide"
     promoter        626..642
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(1242..1258)
                     /vntifkey="30"
                     /label=T3\promoter
     misc_feature    complement(1284..1321)
                     /vntifkey="21"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(1322..1405)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1497..1899)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2440..3300)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             678..1199
                     /vntifkey="4"
                     /label=URA3\fragment
BASE COUNT      856 a       821 c       858 g       893 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcga 
      661 tatcctccaa tactcctttg gagggcacag ttaagccgct aaaggcatta tccgccaagt 
      721 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 
      781 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 
      841 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 
      901 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 
      961 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 
     1021 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 
     1081 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 
     1141 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatc 
     1201 catacaagta ttggagatat cggtacccag cttttgttcc ctttagtgag ggttaattgc 
     1261 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     1321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     1381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     1441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     1501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     1561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     1621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     1681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     1741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     1801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     1861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     1921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     1981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     2041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     2101 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     2161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     2221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     2281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     2341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     2401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     2461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     2521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     2581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     2641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     2701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     2761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     2821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     2881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     2941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     3001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     3061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     3121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     3181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     3241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     3301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     3361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     3421 agtgccac 
//
