LOCUS       pENTR-R2L3-APEX         4339 bp    DNA     circular     28-FEB-2014
DEFINITION  LMBP 9005.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9005
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-FEB-2014
            
COMMENT     VNTDATE|690893934|
COMMENT     VNTNAME|pENTR-R2L3-APEX|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     rep_origin      3348..3750
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1724..2734
                     /vntifkey="4"
                     /label=streptomycin/spectinomycin\resistance\(aadA)
     attenuator      complement(930..1054)
                     /vntifkey="0"
                     /label=attR2
     CDS             complement(179..925)
                     /vntifkey="4"
                     /label=APEX
     promoter        23..39
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      79..174
                     /vntifkey="0"
                     /label=attL3
BASE COUNT     1020 a      1112 c      1150 g      1057 t 
ORIGIN
        1 tcaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcgc ggccgcgtta 
       61 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc 
      121 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttgtttatg 
      181 cctcggcgaa tcccagttca gacagtttca ggtgtgcctc agcatagtcg gcgaagaaca 
      241 catcctcgtc ggctgcatac ttttcgacca gaggcctgaa cacgctatct gtcagcaggg 
      301 ctttgtcaga gggcagctgc agcagtccat ccttctcgcc ggtcagcagt tcagtaaagt 
      361 aggaattgtc gaagatcaga gggttgcttg tccagggtcc ctcgaagcct gagcgttcct 
      421 tatgagcggc gccaatggtg tgccctccac tcagggccac gatatcctgg tcagacagcc 
      481 ccattgcttt tccgaacacg tcccgcagat ggtcgctgcc cttggtagca tctggcagtc 
      541 tcccctctgg agggggttca ggtttgtcct cccttcctgg gtggaaaggg accttggggc 
      601 cgccggtaat ctccacagcg accacgccgg ccagctggta gaagtctgcg taggagacaa 
      661 tggggaactg ttctttgatt ggctccagca gtctcacagc aatgtccagg ccattgttgg 
      721 ccccatgtgc cagttcagcc tggtgcttga tagtcccaaa gggtccgcct gttttggtct 
      781 tggagtcaaa tgttcctgcg ctgtggaaag ccagtcgcag gatcagtggg gcgcatttct 
      841 tttctgcgat gaatcccctc agtttccttt ttgccttctc aatagcgtcc tggtaatctg 
      901 ggctcacggt tgggtatgac tttcccctat ccactttgta caagaaagtt gaacgagaaa 
      961 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat 
     1021 aatactgtaa aacacaacat atccagtcac tatggaattc gagctctaga gctgcagggc 
     1081 ccgagcttaa gactggccgt cgttttacgg taccgcgatc gctcgcgacc tgcaggcata 
     1141 aagccgtcag tgtccgcata aagaaccacc cataataccc ataatagctg tttgccatcg 
     1201 ctaccttagg accgttatag ttaattaccc tgttatccct attaattaag agctcgctac 
     1261 cttaagagag gatatcggcg cgcctctaga atttaaatgg atcctacgta ctcgaggaat 
     1321 tcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 
     1381 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc 
     1441 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca 
     1501 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa 
     1561 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt 
     1621 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa 
     1681 gcctcgttcg gttcgtaagc tgtaatgcaa gtagcgtaac tgccgtcacg caactggtcc 
     1741 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttcttgt 
     1801 tatgacatgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta 
     1861 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg 
     1921 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact 
     1981 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt 
     2041 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac 
     2101 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac 
     2161 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca 
     2221 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca 
     2281 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat 
     2341 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact 
     2401 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg 
     2461 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat 
     2521 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat 
     2581 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg 
     2641 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt 
     2701 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc 
     2761 gacgccgctt cgccggcgtt aactcaagcg attagatgca ctaagcacat aattgctcac 
     2821 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac 
     2881 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact 
     2941 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 
     3001 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 
     3061 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 
     3121 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 
     3181 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 
     3241 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 
     3301 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 
     3361 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 
     3421 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 
     3481 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 
     3541 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 
     3601 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 
     3661 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 
     3721 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 
     3781 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 
     3841 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 
     3901 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     3961 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     4021 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt 
     4081 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg 
     4141 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt 
     4201 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa 
     4261 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat 
     4321 cagtcactta catgtgtga 
//
