LOCUS       pENTR-R2L3-mKate        4285 bp    DNA     circular     28-FEB-2014
DEFINITION  LMBP 9007.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9007
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-FEB-2014
            
            
            
COMMENT     VNTDATE|690896321|
COMMENT     VNTNAME|pENTR-R2L3-mKate|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
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COMMENT                                   "streptomycin/spectinomycin resistance (aadA)" 
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FEATURES             Location/Qualifiers
     rep_origin      3294..3696
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1670..2680
                     /vntifkey="4"
                     /label=streptomycin/spectinomycin\resistance\(aadA)
     attenuator      complement(876..1000)
                     /vntifkey="0"
                     /label=attR2
     CDS             complement(179..874)
                     /vntifkey="4"
                     /label=mKATE
     promoter        23..39
                     /vntifkey="30"
                     /label=T7
     attenuator      79..174
                     /vntifkey="0"
                     /label=attL3
BASE COUNT      987 a      1093 c      1156 g      1049 t 
ORIGIN
        1 tcaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcgc ggccgcgtta 
       61 acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc tgttcgttgc 
      121 aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa gttgtttatc 
      181 tgtgccccag tttgctaggg aggtcgcagt atctggccac agccacctcg tgctgctcga 
      241 cgtaggtctc tttgtcggcc tccttgattc tttccagtct tctgtccaca tagtagacgc 
      301 cgggcatctt gaggttctta gcgggtttct tggatctgta tgtggtcttc aagttgcaga 
      361 tcaggtggcc cccgcccacg agcttcaggg ccatgtcggc tctgccttcc aggccgccgt 
      421 cagcggggta cagggtctcg gtggaggcct cccagccgag tgttttcttc tgcatcacag 
      481 ggccgttgga tgggaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct 
      541 ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct 
      601 cccatgtgaa gccctcgggg aaggactgct taaagaagtc ggggatgccc tgggtgtggt 
      661 tgatgaaggt tttgctgccg tacatgaagc tggtagccag gatgtcgaag gcgaagggga 
      721 gagggccgcc ctcgaccgcc ttgattctca tggtctgggt gccctcgtag ggcttgcctt 
      781 cgccctcgga tgtgcacttg aagtggtggt tgttcacggt gccctccatg tacagcttca 
      841 tgtgcatgtt ctccttaatc agctcgctca ccatggccac tttgtacaag aaagctgaac 
      901 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga 
      961 ctacataata ctgtaaaaca caacatatcc agtcactatg gaattcgagc tctagagctg 
     1021 cagggcccga gcttaagact ggccgtcgtt ttacggtacc gcgatcgctc gcgacctgca 
     1081 ggcataaagc cgtcagtgtc cgcataaaga accacccata atacccataa tagctgtttg 
     1141 ccatcgctac cttaggaccg ttatagttaa ttaccctgtt atccctatta attaagagct 
     1201 cgctacctta agagaggata tcggcgcgcc tctagaattt aaatggatcc tacgtactcg 
     1261 aggaattcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt 
     1321 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 
     1381 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg 
     1441 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg 
     1501 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg 
     1561 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg 
     1621 acataagcct cgttcggttc gtaagctgta atgcaagtag cgtaactgcc gtcacgcaac 
     1681 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct 
     1741 tcttgttatg acatgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc 
     1801 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg 
     1861 cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac 
     1921 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt 
     1981 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct 
     2041 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt 
     2101 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt 
     2161 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga 
     2221 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct 
     2281 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga 
     2341 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac 
     2401 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc 
     2461 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg 
     2521 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta 
     2581 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca 
     2641 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt 
     2701 caagccgacg ccgcttcgcc ggcgttaact caagcgatta gatgcactaa gcacataatt 
     2761 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag 
     2821 ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt 
     2881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     2941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     3001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     3061 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     3121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     3181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     3241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     3301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     3361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     3421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     3481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     3541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     3601 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     3661 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta 
     3721 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg 
     3781 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc 
     3841 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 
     3901 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 
     3961 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg 
     4021 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag 
     4081 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag 
     4141 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg 
     4201 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt 
     4261 ttatatcagt cacttacatg tgtga 
//
