LOCUS       pdcDNA3-R4R3            7003 bp    DNA     circular     28-FEB-2014
DEFINITION  LMBP 9009.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9009
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-FEB-2014
            
COMMENT     VNTDATE|690892142|
COMMENT     VNTNAME|pdcDNA3-R4R3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pdcDNA3-R4R3" 0 7003 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 6583 6582 1 7003 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #1=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6007 6867 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 30 0 0 863 879 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "attR4" 0 0 1 895 1019 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 1707 2366 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "lacUV5 promoter" 30 0 1 2415 2471 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "ccdB" 4 0 1 1060 1365 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "attR3" 0 0 0 2475 2599 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "SP6 promoter" 30 0 1 2630 2646 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "BGH polyA" 43 0 0 2672 2896 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 5064 5466 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "f1 ori" 33 0 0 2942 3370 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3375 3584 0 
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COMMENT        #13=(CFSignal (CObList) "SV40 ori" 33 0 0 3541 3616 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 3708 4502 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4676 4750 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4676 4750 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "lac promoter" 30 0 1 4889 4972 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "Last modified: 28-FEB-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pdcDNA3-R4R3'" 1) 
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COMMENT                          1) 
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COMMENT                          "Created: 02/28/14 10:29" 1) 
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COMMENT                          "Last Modified: 02/28/14 02:39" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7003 bp" 1) 
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COMMENT                          "CDS (4 total)") 
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COMMENT                          "Replication Origin (3 total)") 
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COMMENT                   "Feature Map")))) 
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COMMENT                                   -10 6.7928 1.16519 #169#) 
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FEATURES             Location/Qualifiers
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             complement(6007..6867)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(895..1019)
                     /vntifkey="0"
                     /label=attR4
     CDS             complement(1707..2366)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     promoter        complement(2415..2471)
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             complement(1060..1365)
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2475..2599
                     /vntifkey="0"
                     /label=attR3
     promoter        complement(2630..2646)
                     /vntifkey="30"
                     /label=SP6\promoter
     terminator      2672..2896
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      complement(5064..5466)
                     /vntifkey="33"
                     /label=pMB1\ori
     rep_origin      2942..3370
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3375..3584
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      3541..3616
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             3708..4502
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4676..4750
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4676..4750)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(4889..4972)
                     /vntifkey="30"
                     /label=lac\promoter
BASE COUNT     1695 a      1772 c      1696 g      1840 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctttcaact 
      901 ttgtatagaa aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 
      961 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg 
     1021 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag 
     1081 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat 
     1141 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat 
     1201 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag 
     1261 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag 
     1321 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt 
     1381 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct 
     1441 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc 
     1501 ttaccgaacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc 
     1561 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac 
     1621 atatcagtat atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg 
     1681 gcttttagta agccggatcc tctagattac gccccgccct gccactcatc gcagtactgt 
     1741 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 
     1801 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 
     1861 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 
     1921 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 
     1981 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 
     2041 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 
     2101 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga 
     2161 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 
     2221 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 
     2281 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 
     2341 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga 
     2401 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg 
     2461 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 
     2521 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa 
     2581 ctttattata catagttgac ctcgagcatg catctagagg gccctattct atagtgtcac 
     2641 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 
     2701 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 
     2761 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 
     2821 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 
     2881 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 
     2941 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     3001 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     3061 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt 
     3121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 
     3181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     3241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 
     3301 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     3361 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 
     3421 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     3481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     3541 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 
     3601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 
     3661 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     3721 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     3781 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     3841 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 
     3901 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     3961 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     4021 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     4081 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     4141 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     4201 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 
     4261 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     4321 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     4381 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     4441 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     4501 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 
     4561 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     4621 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     4681 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     4741 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     4801 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     4861 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4921 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4981 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     5041 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     5101 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     5161 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     5221 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     5281 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     5341 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     5401 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 
     5461 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     5521 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     5581 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     5641 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     5701 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     5761 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     5821 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     5881 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     5941 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     6001 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     6061 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     6121 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     6181 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     6241 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     6301 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     6361 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     6421 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     6481 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     6541 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     6601 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     6661 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     6721 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     6781 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     6841 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     6901 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     6961 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc 
//
