LOCUS       pUC57-APC               2913 bp    DNA     circular     25-AUG-2014
DEFINITION  LMBP 9011.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9011
COMMENT     VNTDATE|691867682|
COMMENT     VNTNAME|pUC57-APC|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                        "Created: 03/07/14 05:28" 1) 
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COMMENT                        "Last Modified: 08/25/14 03:27" 1) 
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COMMENT                        "length: 2913 bp" 1) 
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COMMENT                         "CDS (1 total)") 
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COMMENT                                   "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                  "@N" 1 14.6894 -1.74233 0 -10 5.05742 1.16519 
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COMMENT                           (CWidget 32 (7 32 0) 1 2 0 0 Nil -656 100) 
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COMMENT                                  (LOGPEN 0 6 16711680) 8 1 4.77434 1.9 0.082322 
COMMENT                                  1) 
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COMMENT                                   "Arial") 1.74233 0.666667 0 
COMMENT                                  "5' UTR of lacZ, incl. RBS" "@N" 1 17.255 0 0 
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COMMENT                                   "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 1 
COMMENT                                  10.6793 -8.71165 0 -10 3.56994 1.16519 #117#)) 
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COMMENT                    (CObjectList) (CObjectList)) 
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COMMENT                    (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
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FEATURES             Location/Qualifiers
     misc_feature    432..634
                     /vntifkey="21"
                     /label=APC
     CDS             complement(1853..2713)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(910..1312)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(735..735)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(697..697)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
BASE COUNT      724 a       726 c       748 g       715 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga tgatgcttct tggggcttct tgttcaacac cggaaccgaa acatcattgt 
      481 cccgctgttc tcaacacaac gcacgacttt ctgaactcaa agcacccaca attatgccaa 
      541 tgttggtgac tggagtttga gtatatcggc actccatgga aagaagcagg tgtgcggaag 
      601 aaggaatggg ttgcgaggag gttgatgtgg tggtatcgga tcccgggccc gtcgactgca 
      661 gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 
      721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 
      781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 
      841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
      901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
      961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     1021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     1081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     1141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     1201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     1261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     1321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     1381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     1441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     1501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     1561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     1621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     1681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     1741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     1801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     1861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     1921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     1981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     2041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     2101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     2161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     2221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     2281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     2341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     2401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     2461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     2521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     2581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     2641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     2701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     2761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     2821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 
     2881 ataaaaatag gcgtatcacg aggccctttc gtc 
//
