LOCUS       pEF6-FKBP-F-MALT1C        7671 bp    DNA     circular     28-JUN-2013
DEFINITION  LMBP 9036.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|666019908|
COMMENT     VNTNAME|pEF6-FKBP-F-MALT1C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-FKBP-F-MALT1C" 0 7671 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7671 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 2659 0 3905 0 4193 0 
COMMENT            5848 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 22 0 1181 0 4557 0 4659 0 
COMMENT            4845 0 5360 0 5477 0 5894 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 5764 0 6183 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 6120 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 4426 0 5746 0 6139 0 
COMMENT            6680 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 1067 0 1287 0 5768 0 6146 
COMMENT            0 7461 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 4828 0 5333 0 6129 0 7413 
COMMENT            0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 1183 0 5896 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 2 1181 0 5894 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "Kozak" 21 0 0 5769 5774 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "FKBP" 4 0 0 5775 6116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "Ampicilin resistance" 4 0 1 3164 4024 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "bla" 30 0 1 4059 4143 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "pUC ori" 33 0 1 2357 3018 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA" 26 0 0 1844 1974 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "Blasticidin resistance" 4 0 0 1288 1686 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "EM-7" 29 0 0 1221 1287 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "SV40+ori" 29 0 0 858 1166 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "f1 ori" 33 0 0 403 831 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "BGH polyA" 26 0 0 133 357 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "BGH reverse" 28 0 0 127 144 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "His6" 21 0 0 91 144 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "Myc" 4 0 0 42 71 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "T7" 28 0 0 5706 5725 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "pEF1-alpha" 29 0 0 4546 5705 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "casp like" 31 0 0 6219 6611 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "992..1384") 
COMMENT        #27=(CFSignal (CObList) "TRAF6" 31 0 0 7599 7616 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2372..2389") 
COMMENT        #28=(CFSignal (CObList) "PCR artefact" 62 0 0 7139 7139 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1912..1912") 
COMMENT        #29=(CFSignal (CObList) "stability creator" 23 0 0 6677 6677 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1450..1450") 
COMMENT        #30=(CFSignal (CObList) "VDJ4" 31 0 0 7149 7280 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1922..2053") 
COMMENT        #31=(CFSignal (CObList) "flag epitope" 21 0 0 6147 6173 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "920..946") 
COMMENT        #32=(CFSignal (CObList) "MALT1-C" 4 0 0 6189 7661 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "962..2434")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                    "pEF6-FKBP-F-MALT1C" 2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pEF6-FKBP-F-MALT1C'" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/28/13 01:31" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/28/13 01:36" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7671 bp" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   "Standard Fields") 
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COMMENT                   0 0) 
COMMENT              #45=(CFolderPar 
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COMMENT                   "Annotations") 
COMMENT              #46=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 1288  End: 1686" 1))) 
COMMENT                                 "Blasticidin resistance") 
COMMENT                            #52=(CFolderPar 
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COMMENT                                  (CParagraph 3164 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3164  End: 4024 (Complementary)" 
COMMENT                                        1))) "Ampicilin resistance") 
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COMMENT                                  (CParagraph 5775 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5775  End: 6116" 1))) "FKBP") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6189 (3 #32# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6189  End: 7661" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   962..2434" 1))) "MALT1-C"))) 
COMMENT                          "CDS (5 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                        "Start: 91    End: 144 " 1))) "His6") 
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COMMENT                                  (CObjectList 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6147 (3 #31# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6147  End: 6173" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   920..946" 1))) "flag epitope"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #69=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 23 (7 23 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6677 (3 #29# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6677  End: 6677" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1450..1450" 1))) "stability creator"))) 
COMMENT                          "Modified Base (1 total)") 
COMMENT                     #75=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 133 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 133   End: 357 " 1))) 
COMMENT                                 "BGH polyA") 
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COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1844 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1844  End: 1974" 1))) 
COMMENT                                 "SV40 polyA"))) "PolyA Site (2 total)") 
COMMENT                     #80=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #81=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 127 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 127   End: 144 " 1))) 
COMMENT                                 "BGH reverse") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5706 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5706  End: 5725" 1))) "T7"))) 
COMMENT                          "Primer Binding Site (2 total)") 
COMMENT                     #85=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 858 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 858   End: 1166" 1))) "SV40+ori") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1221 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1221  End: 1287" 1))) "EM-7") 
COMMENT                            #90=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4546  End: 5705" 1))) 
COMMENT                                 "pEF1-alpha"))) "Promoter Eukaryotic (3 total)") 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
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COMMENT                                        "Start: 4059  End: 4143 (Complementary)" 
COMMENT                                        1))) "bla"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #95=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 31 (7 31 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6219  End: 6611" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   992..1384" 1))) "casp like") 
COMMENT                            #100=(CFolderPar 
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COMMENT                                   (CObjectList 
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COMMENT                                          "Start: 7149  End: 7280" 1) 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   1922..2053" 1))) "VDJ4") 
COMMENT                            #104=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 7599 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #105=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 7599  End: 7616" 1) 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   2372..2389" 1))) "TRAF6"))) 
COMMENT                          "Protein Binding Site (3 total)") 
COMMENT                     #108=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #109=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 403 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 403   End: 831 " 1))) 
COMMENT                                   "f1 ori") 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2357 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2357  End: 3018 (Complementary)" 
COMMENT                                           1))) "pUC ori"))) 
COMMENT                           "Replication Origin (2 total)") 
COMMENT                     #113=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 62 (7 62 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #114=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7139 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7139  End: 7139" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "silent mutation, G replaces A" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1912..1912" 1))) "PCR artefact"))) 
COMMENT                           "Mutation (1 total)"))) "Feature Map") 
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COMMENT                     (CObjectList 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24745072 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2659 " 1) 
COMMENT                                      #123=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3905 " 1) 
COMMENT                                      #124=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4193 " 1) 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5848 " 1))))) "ApaLI: 4 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
COMMENT                      #126=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24745040 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #127=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 22 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1181 " 1) 
COMMENT                                      #130=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4557 " 1) 
COMMENT                                      #131=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4659 " 1) 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 4845 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 5360 " 1) 
COMMENT                                      #134=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 5477 " 1) 
COMMENT                                      #135=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 5894 " 1))))) "AvaI: 8 sites") 
COMMENT                            1 5 "CYCGRG" "GRGCYC") 
COMMENT                      #136=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 24745008 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #137=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5764 " 1) 
COMMENT                                      #139=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
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COMMENT                            1 5 "GGATCC" "CCTAGG") 
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FEATURES             Location/Qualifiers
     misc_feature    5769..5774
                     /vntifkey="21"
                     /label=Kozak
     CDS             5775..6116
                     /vntifkey="4"
                     /label=FKBP
     CDS             complement(3164..4024)
                     /vntifkey="4"
                     /label=Ampicilin\resistance
     promoter        complement(4059..4143)
                     /vntifkey="30"
                     /label=bla
     rep_origin      complement(2357..3018)
                     /vntifkey="33"
                     /label=pUC\ori
     polyA_site      1844..1974
                     /vntifkey="26"
                     /label=SV40\polyA
     CDS             1288..1686
                     /vntifkey="4"
                     /label=Blasticidin\resistance
     promoter        1221..1287
                     /vntifkey="29"
                     /label=EM-7
     promoter        858..1166
                     /vntifkey="29"
                     /label=SV40+ori
     rep_origin      403..831
                     /vntifkey="33"
                     /label=f1\ori
     polyA_site      133..357
                     /vntifkey="26"
                     /label=BGH\polyA
     primer_bind     127..144
                     /vntifkey="28"
                     /label=BGH\reverse
     misc_feature    91..144
                     /vntifkey="21"
                     /label=His6
     CDS             42..71
                     /vntifkey="4"
                     /label=Myc
     primer_bind     5706..5725
                     /vntifkey="28"
                     /label=T7
     promoter        4546..5705
                     /vntifkey="29"
                     /label=pEF1-alpha
     protein_bind    6219..6611
                     /vntifkey="31"
                     /label=casp\like
     protein_bind    7599..7616
                     /vntifkey="31"
                     /label=TRAF6
     mutation        7139..7139
                     /vntifkey="62"
                     /label=PCR\artefact
                     /note="silent mutation, G replaces A"
     modified_base   6677..6677
                     /vntifkey="23"
                     /label=stability\creator
                     /note="T replaces A as GCAGAAG recombines with the same heptanucleotide present in API2"
     protein_bind    7149..7280
                     /vntifkey="31"
                     /label=VDJ4
     misc_feature    6147..6173
                     /vntifkey="21"
                     /label=flag\epitope
     CDS             6189..7661
                     /vntifkey="4"
                     /label=MALT1-C
BASE COUNT     1860 a      1841 c      1984 g      1986 t 
ORIGIN
        1 atccagcaca gtggcggccg ctcgagtcta gagggccctt cgaacaaaaa ctcatctcag 
       61 aagaggatct gaatatgcat accggtcatc atcaccatca ccattgagtt taaacccgct 
      121 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
      181 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
      241 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
      301 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
      361 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
      421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
      481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
      541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
      601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
      661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
      721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
      781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
      841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
      901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
      961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     1021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     1081 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     1141 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     1201 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta 
     1261 taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc 
     1321 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc 
     1381 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt 
     1441 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc 
     1501 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga 
     1561 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt 
     1621 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag 
     1681 ggctaagcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 
     1741 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     1801 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     1861 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     1921 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     1981 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     2041 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     2101 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     2161 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     2221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     2281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     2341 taacgcagga aagaacatgt gagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     2401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     2461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     2521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     2581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     2641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     2701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     2761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     2821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 
     2881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     2941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     3001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     3061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     3121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     4141 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct 
     4201 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 
     4261 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 
     4321 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc 
     4381 cagatatacg cgttgacatt gattattgac taggcttttg caaaaagctt tgcaaagatg 
     4441 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg 
     4501 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga 
     4561 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa 
     4621 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta 
     4681 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca 
     4741 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt 
     4801 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg 
     4861 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt 
     4921 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg 
     4981 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct 
     5041 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt 
     5101 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg 
     5161 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc 
     5221 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg 
     5281 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc 
     5341 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag 
     5401 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag 
     5461 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt 
     5521 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca 
     5581 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa 
     5641 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt 
     5701 ggtactaata cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc 
     5761 tcggatccat ggaaagagga gtgcaggtgg agactatctc cccaggagac gggcgcacct 
     5821 tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga 
     5881 aagttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg 
     5941 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga 
     6001 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg 
     6061 ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagtggaggt ggaggttgga 
     6121 attctgcaga taaacttaag cttaccatgg attacaagga cgatgacgat aaggtaccta 
     6181 aggatcctac aactgaccag cctttggcga aggacaaggt tgcccttttg ataggaaata 
     6241 tgaattaccg ggagcacccc aagctcaaag ctcctttggt ggatgtgtac gaattgacta 
     6301 acttactgag acagctggac ttcaaagtgg tttcactgtt ggatcttact gaatatgaga 
     6361 tgcgtaatgc tgtggatgag tttttactcc ttttagacaa gggagtatat gggttattat 
     6421 attatgcagg acatggttat gaaaattttg ggaacagctt catggtcccc gttgatgctc 
     6481 caaatccata taggtctgaa aattgtctgt gtgtacaaaa tatactgaaa ttgatgcaag 
     6541 aaaaagaaac tggacttaat gtgttcttat tggatatgtg taggaaaaga aatgactacg 
     6601 atgataccat tccaatcttg gatgcactaa aagtcaccgc caatattgtg tttggatatg 
     6661 ccacgtgtca aggagctgaa gcttttgaaa tccagcattc tggattggca aatggaatct 
     6721 ttatgaaatt tttaaaagac agattattag aagataagaa aatcactgtg ttactggatg 
     6781 aagttgcaga agatatgggt aagtgtcacc ttaccaaagg caaacaggct ctagagattc 
     6841 gaagtagttt atctgagaag agagcactta ctgatccaat acagggaaca gaatattctg 
     6901 ctgaatctct tgtgcggaat ctacagtggg ccaaggctca tgaacttcca gaaagtatgt 
     6961 gtcttaagtt tgactgtggt gttcagattc aattaggatt tgcagctgag ttttccaatg 
     7021 tcatgatcat ctatacaagt atagtttaca aaccaccgga gataataatg tgtgatgcct 
     7081 acgttactga ttttccactt gatctagata ttgatccaaa agatgcaaat aaaggcacgc 
     7141 ctgaagaaac tggcagctac ttggtatcaa aggatcttcc caagcattgc ctctatacca 
     7201 gactcagttc actgcaaaaa ttaaaggaac atctagtctt cacagtatgt ttatcatatc 
     7261 agtactcagg attggaagat actgtagagg acaagcagga agtgaatgtt gggaaacctc 
     7321 tcattgctaa attagacatg catcgaggtt tgggaaggaa gacttgcttt caaacttgtc 
     7381 ttatgtctaa tggtccttac cagagttctg cagccacctc aggaggagca gggcattatc 
     7441 actcattgca agacccattc catggtgttt accattcaca tcctggtaat ccaagtaatg 
     7501 ttacaccagc agatagctgt cattgcagcc ggactccaga tgcatttatt tcaagtttcg 
     7561 ctcaccatgc ttcatgtcat tttagtagaa gtaatgtgcc agtagagaca actgatgaaa 
     7621 taccatttag tttctctgac aggctcagaa tttctgaaaa agggcccgtt t 
//

