LOCUS       pENTR221-CnA-dCAM-AI        3724 bp    DNA     circular      1-JUL-2013
DEFINITION  LMBP 9038.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|666458434|
COMMENT     VNTNAME|pENTR221-CnA-dCAM-AI|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
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COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 1546 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2426 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3335 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 3496 0 3706 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
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COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "attL2" 21 0 1 3717 88 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 0 2133 
COMMENT             1 "") 
COMMENT        #13=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 0 2292 
COMMENT             1 "") 
COMMENT        #14=(CFSignal (CObList) "T7 promoter" 30 0 1 109 125 0 (CStringList) 
COMMENT        #15=(CFSignal (CObList) "T7 primer" 27 0 1 106 125 0 (CStringList) 
COMMENT        #16=(CFSignal (CObList) "M13 reverse primer" 27 0 1 130 146 0 
COMMENT        #17=(CFSignal (CObList) "M13 (-40) forward primer" 27 0 0 2382 2398 0 
COMMENT        #18=(CFSignal (CObList) "M13 (-20) forward primer" 27 0 0 2402 2417 0 
COMMENT        #19=(CFSignal (CObList) "Kan(R)" 4 0 0 259 1068 0 (CStringList) 
COMMENT        #20=(CFSignal (CObList) "G" 21 0 0 3713 3713 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "GG" 21 0 0 2534 2535 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "CnA-dCAM-AI" 4 0 0 2536 3712 0 (CStringList) 
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COMMENT                          "Created: 07/01/13 03:20" 1) 
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COMMENT                          "length: 3724 bp" 1) 
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COMMENT                          "If necessary, linearize attB sequence attB1-hSTAT1-attB2 at a unique REN site outside the attB region. Ethanol precipitate and resuspend." 
COMMENT                          1) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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COMMENT                          1) 
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COMMENT                          "Select Entry Clones on agar plates with antibiotic kanamycin." 
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FEATURES             Location/Qualifiers
     misc_feature    2435..2530
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(3717..88)
                     /vntifkey="21"
                     /label=attL2
     terminator      2133..2160
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /vntifkey="4"
                     /label=Kan(R)
     misc_feature    3713..3713
                     /vntifkey="21"
                     /label=G
     misc_feature    2534..2535
                     /vntifkey="21"
                     /label=GG
     CDS             2536..3712
                     /vntifkey="4"
                     /label=CnA-dCAM-AI
BASE COUNT      985 a       844 c       890 g      1005 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gctggatgtc cgagcccaag gcaattgatc ccaagttgtc gacgaccgac 
     2581 agggtggtga aagctgttcc atttcctcca agtcaccggc ttacagcaaa agaagtgttt 
     2641 gataatgatg gaaaacctcg tgtggatatc ttaaaggcgc atcttatgaa ggagggaagg 
     2701 ctggaagaga gtgttgcatt gagaataata acagagggtg catcaattct tcgacaggaa 
     2761 aaaaatttgc tggatattga tgcgccagtc actgtttgtg gggacattca tggacaattc 
     2821 tttgatttga tgaagctctt tgaagtcggg ggatctcctg ccaacactcg ctacctcttc 
     2881 ttaggggact atgttgacag agggtacttc agtattgaat gtgtgctgta tttgtgggcc 
     2941 ttgaaaattc tctaccccaa aacactgttt ttacttcgtg gaaatcatga atgtagacat 
     3001 ctaacagagt atttcacatt taaacaagaa tgtaagataa agtattcaga acgcgtatat 
     3061 gatgcctgta tggatgcctt tgactgcctt cccctggctg ccctgatgaa ccaacagttc 
     3121 ctgtgtgtgc atggtggttt gtctccagag attaacactt tagatgatat cagaaaatta 
     3181 gaccgattca aagaaccacc tgcatatgga cctatgtgtg atatcctgtg gtcagacccc 
     3241 ctggaagatt ttggaaatga gaagactcag gaacatttca ctcacaacac agtcaggggg 
     3301 tgttcatact tctacagtta cccggctgta tgtgaattct tacagcacaa taacttgtta 
     3361 tctatactcc gagcccacga agcccaagat gcagggtacc gcatgtacag gaaaagccaa 
     3421 acaacaggct tcccttctct aattacaatt ttttcagcac caaattactt agatgtatac 
     3481 aataacaaag ctgcagtatt gaagtatgag aacaatgtta tgaatatcag gcaattcaac 
     3541 tgttctcctc atccatactg gcttccaaat ttcatggatg tttttacttg gtcccttcca 
     3601 tttgttgggg aaaaagtgac tgagatgctg gtaaatgtcc tcaacatctg ctcagatgat 
     3661 gaactagggt cagaagaaga tggatttgat ggtgcaacag ctgcagcccg gcgacccagc 
     3721 tttc 
//

