LOCUS       pENT-miR125a            2347 bp    DNA     circular     14-APR-2014
DEFINITION  LMBP 9051.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9051
            Last modified: 14-APR-2014
COMMENT     VNTDATE|695208192|
COMMENT     VNTNAME|pENT-miR125a|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENT-miR125a" 0 2347 
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COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT         "DraI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
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COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT        #1=(CFSignal (CObList) "T1 terminator" 43 0 1 106 149 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(106..149)") 
COMMENT        #2=(CFSignal (CObList) "T2 terminator" 43 0 0 281 308 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "281..308") 
COMMENT        #3=(CFSignal (CObList) "pMB1 ori" 33 0 0 2067 45 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2020..45") 
COMMENT        #4=(CFSignal (CObList) "miR125a" 4 0 0 460 527 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "460..527") 
COMMENT        #5=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 798 1607 0 
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COMMENT        #6=(CFSignal (CObList) "attL2" 0 0 1 532 627 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "Last modified: 14-APR-2014" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pENT-miR125a'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 04/14/14 09:23" 1) 
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COMMENT                          "Last Modified: 04/14/14 09:23" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     attenuator      359..454
                     /vntifkey="0"
                     /label=attL1
     terminator      complement(106..149)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      281..308
                     /vntifkey="43"
                     /label=T2\terminator
     rep_origin      2067..45
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             460..527
                     /vntifkey="4"
                     /label=miR125a
     CDS             798..1607
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     attenuator      complement(532..627)
                     /vntifkey="0"
                     /label=attL2
     promoter        complement(648..664)
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT      597 a       526 c       579 g       644 t         1 others
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttac tgggtccctg agacccttta 
      481 acctgtgagg acgtccaggg tcacaggtga ggttcttggg agcctgggac ccagctttct 
      541 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca 
      601 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgmgtcgt 
      661 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt 
      721 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 
      781 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 
      841 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 
      901 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 
      961 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 
     1021 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 
     1081 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 
     1141 gtgaaaatat tgttgatgcg ctggcagtgt ccctgcgccg gttgcattcg attcctgttt 
     1201 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 
     1261 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 
     1321 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 
     1381 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 
     1441 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 
     1501 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 
     1561 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 
     1621 attggttgta acattattca gattgggccc cgttccactg agcgtcagac cccgtagaaa 
     1681 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     1741 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     1801 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     1861 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     1921 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     1981 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     2041 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     2101 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     2161 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     2221 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     2281 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     2341 acatgtt 
//
