LOCUS       pENT-hA20-ZF4/7-mut        4706 bp    DNA     circular     14-APR-2014
DEFINITION  LMBP 9056.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9056
            Last modified: 14-APR-2014
            
            
            
            
COMMENT     VNTDATE|695215962|
COMMENT     VNTNAME|pENT-hA20 ZF4/7 mut|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENT-hA20 ZF4/7 mut" 0 4706 
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COMMENT         "DraI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "complement(106..149)") 
COMMENT        #2=(CFSignal (CObList) "T2 terminator" 43 0 0 281 308 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "281..308") 
COMMENT        #3=(CFSignal (CObList) "pMB1 ori" 33 0 0 4426 45 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4379..45") 
COMMENT        #4=(CFSignal (CObList) "hA20 mutant" 4 0 0 514 2886 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "514..2886") 
COMMENT        #5=(CFSignal (CObList) "C624A" 62 0 0 2383 2385 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "C627A" 62 0 0 2392 2394 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "C775A" 62 0 0 2836 2838 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "C779A" 62 0 0 2848 2850 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 3157 3966 0 
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COMMENT        #11=(CFSignal (CObList) "attL2" 0 0 1 2891 2986 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pENT-hA20 ZF4/7 mut'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 04/14/14 11:32" 1) 
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COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4706 bp" 1) 
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FEATURES             Location/Qualifiers
     attenuator      359..454
                     /vntifkey="0"
                     /label=attL1
     terminator      complement(106..149)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      281..308
                     /vntifkey="43"
                     /label=T2\terminator
     rep_origin      4426..45
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             514..2886
                     /vntifkey="4"
                     /label=hA20\mutant
     mutation        2383..2385
                     /vntifkey="62"
                     /label=C624A
                     /note="WT: TGC"
     mutation        2392..2394
                     /vntifkey="62"
                     /label=C627A
                     /note="WT: TGT"
     misc_marker     466..504
                     /vntifkey="22"
                     /label=E-tag
     mutation        2836..2838
                     /vntifkey="62"
                     /label=C775A
     mutation        2848..2850
                     /vntifkey="62"
                     /label=C779A
     CDS             3157..3966
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     attenuator      complement(2891..2986)
                     /vntifkey="0"
                     /label=attL2
     promoter        complement(3007..3023)
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT     1230 a      1183 c      1166 g      1126 t         1 others
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttaa ccatgggtgc gccggtgccg 
      481 tatccggacc cgctggaacc gcgtgcggcc gcaatggctg aacaagtcct tcctcaggct 
      541 ttgtatttga gcaatatgcg gaaagctgtg aagatacggg agagaactcc agaagacatt 
      601 tttaaaccta ctaatgggat cattcatcat tttaaaacca tgcaccgata cacactggaa 
      661 atgttcagaa cttgccagtt ttgtcctcag tttcgggaga tcatccacaa agccctcatc 
      721 gacagaaaca tccaggccac cctggaaagc cagaagaaac tcaactggtg tcgagaagtc 
      781 cggaagcttg tggcgctgaa aacgaacggt gacggcaatt gcctcatgca tgccacttct 
      841 cagtacatgt ggggcgttca ggacacagac ttggtactga ggaaggcgct gttcagcacg 
      901 ctcaaggaaa cagacacacg caactttaaa ttccgctggc aactggagtc tctcaaatct 
      961 caggaatttg ttgaaacggg gctttgctat gatactcgga actggaatga tgaatgggac 
     1021 aatcttatca aaatggcttc cacagacaca cccatggccc gaagtggact tcagtacaac 
     1081 tcactggaag aaatacacat atttgtcctt tgcaacatcc tcagaaggcc aatcattgtc 
     1141 atttcagaca aaatgctaag aagtttggaa tcaggttcca atttcgcccc tttgaaagtg 
     1201 ggtggaattt acttgcctct ccactggcct gcccaggaat gctacagata ccccattgtt 
     1261 ctcggctatg acagccatca ttttgtaccc ttggtgaccc tgaaggacag tgggcctgaa 
     1321 atccgagctg ttccacttgt taacagagac cggggaagat ttgaagactt aaaagttcac 
     1381 tttttgacag atcctgaaaa tgagatgaag gagaagctct taaaagagta cttaatggtg 
     1441 atagaaatcc ccgtccaagg ctgggaccat ggcacaactc atctcatcaa tgccgcaaag 
     1501 ttggatgaag ctaacttacc aaaagaaatc aatctggtag atgattactt tgaacttgtt 
     1561 cagcatgagt acaagaaatg gcaggaaaac agcgagcagg ggaggagaga ggggcacgcc 
     1621 cagaatccca tggaaccttc cgtgccccag ctttctctca tggatgtaaa atgtgaaacg 
     1681 cccaactgcc ccttcttcat gtctgtgaac acccagcctt tatgccatga gtgctcagag 
     1741 aggcggcaaa agaatcaaaa caaactccca aagctgaact ccaagccggg ccctgagggg 
     1801 ctccctggca tggcgctcgg ggcctctcgg ggagaagcct atgagccctt ggcgtggaac 
     1861 cctgaggagt ccactggggg gcctcattcg gccccaccga cagcacccag cccttttctg 
     1921 ttcagtgaga ccactgccat gaagtgcagg agccccggct gccccttcac actgaatgtg 
     1981 cagcacaacg gattttgtga acgttgccac aacgcccggc aacttcacgc cagccacgcc 
     2041 ccagaccaca caaggcactt ggatcccggg aagtgccaag cctgcctcca ggatgttacc 
     2101 aggacattta atgggatctg cagtacttgc ttcaaaagga ctacagcaga ggcctcctcc 
     2161 agcctcagca ccagcctccc tccttcctgt caccagcgtt ccaagtcaga tccctcgcgg 
     2221 ctcgtccgga gcccctcccc gcattcttgc cacagagctg gaaacgacgc ccctgctggc 
     2281 tgcctgtctc aagctgcacg gactcctggg gacaggacgg ggacgagcaa gtgcagaaaa 
     2341 gccggctgcg tgtattttgg gactccagaa aacaagggct ttgccacact ggctttcatc 
     2401 gagtacagag aaaacaaaca ttttgctgct gcctcaggga aagtcagtcc cacagcgtcc 
     2461 aggttccaga acaccattcc gtgcctgggg agggaatgcg gcacccttgg aagcaccatg 
     2521 tttgaaggat actgccagaa gtgtttcatt gaagctcaga atcagagatt tcatgaggcc 
     2581 aaaaggacag aagagcaact gagatcgagc cagcgcagag atgtgcctcg aaccacacaa 
     2641 agcacctcaa ggcccaagtg cgcccgggcc tcctgcaaga acatcctggc ctgccgcagc 
     2701 gaggagctct gcatggagtg tcagcatccc aaccagagga tgggccctgg ggcccaccgg 
     2761 ggtgagcctg cccccgaaga cccccccaag cagcgttgcc gggcccccgc ctgtgatcat 
     2821 tttggcaatg ccaaggccaa cggctacgcc aacgaatgct ttcagttcaa gcagatgtat 
     2881 ggctaatacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt 
     2941 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat 
     3001 atcccctata gtgmgtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg 
     3061 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa 
     3121 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 
     3181 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 
     3241 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca 
     3301 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 
     3361 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 
     3421 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta 
     3481 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtc cctgcgccgg 
     3541 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 
     3601 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 
     3661 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 
     3721 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 
     3781 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 
     3841 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 
     3901 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 
     3961 ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga 
     4021 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     4081 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     4141 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     4201 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     4261 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     4321 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     4381 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     4441 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     4501 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     4561 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     4621 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     4681 ttttgctggc cttttgctca catgtt 
//
