LOCUS       Cre-excised RMCE-hA20 ZF4/7 mut        8175 bp    DNA     circular UNA 14-APR-2014
DEFINITION  LMBP 9064.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9064
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-APR-2014
            
            
            
COMMENT     VNTDATE|695232960|
COMMENT     VNTNAME|Cre excised RMCE-hA20 ZF4/7 mut|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT                          "Created: 04/14/14 04:16" 1) 
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COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
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COMMENT                            1.74233 0.722222 0 "Cre excised RMCE-hA20 ZF4/7 mut" 
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COMMENT                             #204=(CWideArrow 
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COMMENT                             #205=(CLabel 
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COMMENT                             #207=(CLabel 
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COMMENT                            (CObjectList 
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COMMENT                                   "chicken \337-globin 5'HS4 insulator core sequence" 
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COMMENT                                   "chicken \337-globin 5'HS4 insulator core sequence" 
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COMMENT                                   #211#) 
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COMMENT                                   #213#) 
COMMENT                             #215=(CScratch 
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COMMENT                             #217=(CScratch 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
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COMMENT                            (CObjectList)) 
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COMMENT                            (CObjectList 
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COMMENT                             #221=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 70 26 0 0 400 0 1 0 0 3 2 1 34 
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COMMENT                                   -2.74739 -13.4689 0 -10 2.4626 1.15693 #220#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #222=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -667 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #171# 0 100) 
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COMMENT                                    0.997237 5.47052 5.86034 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #224=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 70 26 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mPGK1 promoter" 
COMMENT                                   "@N" 1 11.149 11.3065 0 -10 7.70182 1.15693 
COMMENT                                   #223#)) (CObjectList)) 
COMMENT                      #225=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -979 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #176# 0 100) 
COMMENT                                    (LOGPEN 0 2 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.28482 5.34928 1 0.082322) 0.8 1.8 1) 
COMMENT                             #227=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #174# 28243780 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 70 26 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 13.6253 8.20194 0 -10 5.55324 1.15693 
COMMENT                                   #226#)) (CObjectList)) 
COMMENT                      #228=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -874 100) 
COMMENT                            (CObjectList 
COMMENT                             #229=(CLine 
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COMMENT                                    100) (LOGPEN 0 8 16711680) 8 0.835356 
COMMENT                                   0.791918 1.17176) 
COMMENT                             #230=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #175# 28451164 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 70 26 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of the EMCV polyprotein gene (IRES)" 
COMMENT                                   "@N" 1 -21.2226 8.29152 0 -10 19.8165 1.15693 
COMMENT                                   #229#)) (CObjectList)) 
COMMENT                      #231=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -140 100) 
COMMENT                            (CObjectList 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #146# 0 100) 
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COMMENT                                    4.90575 5.21499 1 0.082322) 0.8 1.8 1) 
COMMENT                             #233=(CLabel 
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FEATURES             Location/Qualifiers
     misc_feature    35..277
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    278..520
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    1070..1117
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(2323..3183)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3801..3848
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4031..4064)
                     /vntifkey="21"
                     /label=loxP
     RBS             6649..7143
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             7156..7875
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(1205..1288)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1380..1782)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        539..1046
                     /vntifkey="29"
                     /label=mPGK1\promoter
     terminator      7906..8130
                     /vntifkey="43"
                     /label=BGH\polyA
     attenuator      4120..4144
                     /vntifkey="0"
                     /label=attB1
     attenuator      6581..6605
                     /vntifkey="0"
                     /label=attB2
     misc_marker     4156..4194
                     /vntifkey="22"
                     /label=E-tag
     CDS             4204..6576
                     /vntifkey="4"
                     /label=hA20\mutant
     mutation        6073..6075
                     /vntifkey="62"
                     /label=C624A
     mutation        6082..6084
                     /vntifkey="62"
                     /label=C627A
     mutation        6526..6528
                     /vntifkey="62"
                     /label=C775A
     mutation        6538..6540
                     /vntifkey="62"
                     /label=C779A
BASE COUNT     1955 a      2279 c      2165 g      1776 t 
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 
     4081 cagcccaagc tagcttatcg ataccgtcga cctcgaatca caagtttgta caaaaaagca 
     4141 ggctgcttaa ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
     4201 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg 
     4261 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat 
     4321 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag 
     4381 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc 
     4441 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt 
     4501 gacggcaatt gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac 
     4561 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa 
     4621 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat 
     4681 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca 
     4741 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt 
     4801 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa 
     4861 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct 
     4921 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc 
     4981 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac 
     5041 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag 
     5101 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat 
     5161 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc 
     5221 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac 
     5281 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag 
     5341 ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac 
     5401 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca 
     5461 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg 
     5521 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg 
     5581 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg 
     5641 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac 
     5701 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg 
     5761 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc 
     5821 ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc tccttcctgt 
     5881 caccagcgtt ccaagtcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc 
     5941 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg 
     6001 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa 
     6061 aacaagggct ttgccacact ggctttcatc gagtacagag aaaacaaaca ttttgctgct 
     6121 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg 
     6181 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt 
     6241 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc 
     6301 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc 
     6361 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc 
     6421 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag 
     6481 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaaggccaa cggctacgcc 
     6541 aacgaatgct ttcagttcaa gcagatgtat ggctaatacc accactttgt acaagaaagc 
     6601 tgggtgattc gaggtcgacg gtatcgataa gcttgatatc gaattccgcc cctctccctc 
     6661 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgag cgtttgtcta 
     6721 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgaa acctggccct 
     6781 gtcttcttga cgacgattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 
     6841 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaca acgtctgtag 
     6901 cgaccctttg caggcagcgg aaccccccac ctggcgacat ggatagttgt ggaaagagtc 
     6961 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 
     7021 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 
     7081 aaaaacgtct aggccccccg aaccacgggg acgtggtttt ggtttgaaaa acacgatgat 
     7141 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 
     7201 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 
     7261 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 
     7321 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 
     7381 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 
     7441 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 
     7501 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 
     7561 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 
     7621 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 
     7681 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 
     7741 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 
     7801 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 
     7861 gagctgtaca agtaaagcgg ccgcgagctc gctgatcagc ctcgactgtg ccttctagtt 
     7921 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
     7981 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
     8041 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
     8101 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
     8161 cgatcctcta gttgg 
//
