LOCUS       pENTR221-hMALT1-WT        5027 bp    DNA     circular     21-NOV-2013
DEFINITION  LMBP 9104.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|679242277|
COMMENT     VNTDBDATE|679242277|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR221-hMALT1-WT|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3131 0 0 "") 
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COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
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COMMENT        #15=(CFSignal (CObList) "T7 primer" 27 0 1 106 125 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "M13 reverse primer" 27 0 1 130 146 0 
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COMMENT        #17=(CFSignal (CObList) "M13 (-40) forward primer" 27 0 0 2382 2398 0 
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COMMENT        #18=(CFSignal (CObList) "M13 (-20) forward primer" 27 0 0 2402 2417 0 
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COMMENT        #19=(CFSignal (CObList) "Kan(R)" 4 0 0 259 1068 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "hMALT1-WT" 4 0 0 2536 5010 0 (CStringList) 
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COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
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COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
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COMMENT                          "Select Entry Clones on agar plates with antibiotic kanamycin." 
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FEATURES             Location/Qualifiers
     misc_feature    2435..2530
                     /vntifkey="21"
                     /label=attL1
     misc_feature    complement(5020..88)
                     /vntifkey="21"
                     /label=attL2
     terminator      2133..2160
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      2292..2335
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     promoter        complement(109..125)
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          complement(106..125)
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     primer          complement(130..146)
                     /invitrogen="2010000"
                     /vntifkey="27"
                     /label=M13\reverse\primer
     primer          2382..2398
                     /invitrogen="2000000"
                     /vntifkey="27"
                     /label=M13\(-40)\forward\primer
     primer          2402..2417
                     /invitrogen="1980000"
                     /vntifkey="27"
                     /label=M13\(-20)\forward\primer
     CDS             259..1068
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     CDS             2536..5010
                     /vntifkey="4"
                     /label=hMALT1-WT
     misc_feature    2534..2535
                     /vntifkey="21"
                     /label=CC
     misc_feature    5008..5010
                     /vntifkey="21"
                     /label=Stop\codon
     misc_feature    5011..5016
                     /vntifkey="21"
                     /label=barcode\sequence\'WT'
BASE COUNT     1365 a      1109 c      1212 g      1341 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg 
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 
      181 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac 
      241 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 
      301 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 
      361 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 
      421 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 
      481 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 
      541 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 
      601 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 
      661 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 
      721 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 
      781 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 
      841 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 
      901 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 
      961 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 
     1021 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 
     1081 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 
     1141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt 
     2101 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct 
     2161 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt 
     2221 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg 
     2281 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat 
     2341 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt 
     2401 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata 
     2461 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac 
     2521 aaaaaagcag gctccatggc gctgttgggg gacccgctac aggccctgcc gccctcggcc 
     2581 gcccccacgg ggccgctgct cgcccctccg gccggcgcga ccctcaaccg cctgcgggag 
     2641 ccgctgctgc ggaggctcag cgagctcctg gatcaggcgc ccgagggccg gggctggagg 
     2701 agactggcgg agctggcggg gagtcgcggg cgcctccgcc tcagttgcct agacctggag 
     2761 cagtgttctc ttaaggtact ggagcctgaa ggaagcccca gcctgtgtct gctgaagtta 
     2821 atgggtgaaa aaggttgcac agtcacagaa ttgagtgatt tcctgcaggc tatggaacac 
     2881 actgaagttc ttcagcttct cagcccccca ggaataaaga ttactgtaaa cccagagtca 
     2941 aaggcagtct tggctggaca gtttgtgaaa ctgtgttgcc gggcaactgg acatcctttt 
     3001 gttcaatatc agtggttcaa aatgaataaa gagattccaa atggaaatac atcagagctt 
     3061 atttttaatg cagtgcatgt aaaagatgca ggcttttatg tctgtcgagt taataacaat 
     3121 ttcacctttg aattcagcca gtggtcacag ctggatgttt gcgacatccc agagagcttc 
     3181 cagagaagtg ttgatggcgt ctctgaatcc aagttgcaaa tctgtgttga accaacttcc 
     3241 caaaagctga tgccaggcag cacattggtt ttacagtgtg ttgctgttgg aagccctatt 
     3301 cctcactacc agtggttcaa aaatgaatta ccattaacac atgagaccaa aaagctatac 
     3361 atggtgcctt atgtggattt ggaacaccaa ggaacctact ggtgtcatgt atataatgat 
     3421 cgagacagtc aagatagcaa gaaggtagaa atcatcatag gaagaacaga tgaggcagtg 
     3481 gagtgcactg aagatgaatt aaataatctt ggtcatcctg ataataaaga gcaaacaact 
     3541 gaccagcctt tggcgaagga caaggttgcc cttttgatag gaaatatgaa ttaccgggag 
     3601 caccccaagc tcaaagctcc tttggtggat gtgtacgaat tgactaactt actgagacag 
     3661 ctggacttca aagtggtttc actgttggat cttactgaat atgagatgcg taatgctgtg 
     3721 gatgagtttt tactcctttt agacaaggga gtatatgggt tattatatta tgcaggacat 
     3781 ggttatgaaa attttgggaa cagcttcatg gtccccgttg atgctccaaa tccatatagg 
     3841 tctgaaaatt gtctgtgtgt acaaaatata ctgaaattga tgcaagaaaa agaaactgga 
     3901 cttaatgtgt tcttattgga tatgtgtagg aaaagaaatg actacgatga taccattcca 
     3961 atcttggatg cactaaaagt caccgccaat attgtgtttg gatatgccac gtgtcaagga 
     4021 gctgaagctt ttgaaatcca gcattctgga ttggcaaatg gaatctttat gaaattttta 
     4081 aaagacagat tattagaaga taagaaaatc actgtgttac tggatgaagt tgcagaagat 
     4141 atgggtaagt gtcaccttac caaaggcaaa caggctctag agattcgaag tagtttatct 
     4201 gagaagagag cacttactga tccaatacag ggaacagaat attctgctga atctcttgtg 
     4261 cggaatctac agtgggccaa ggctcatgaa cttccagaaa gtatgtgtct taagtttgac 
     4321 tgtggtgttc agattcaatt aggatttgca gctgagtttt ccaatgtcat gatcatctat 
     4381 acaagtatag tttacaaacc accggagata ataatgtgtg atgcctacgt tactgatttt 
     4441 ccacttgatc tagatattga tccaaaagat gcaaataaag gcacgcctga agaaactggc 
     4501 agctacttgg tatcaaagga tcttcccaag cattgcctct ataccagact cagttcactg 
     4561 caaaaattaa aggaacatct agtcttcaca gtatgtttat catatcagta ctcaggattg 
     4621 gaagatactg tagaggacaa gcaggaagtg aatgttggga aacctctcat tgctaaatta 
     4681 gacatgcatc gaggtttggg aaggaagact tgctttcaaa cttgtcttat gtctaatggt 
     4741 ccttaccaga gttctgcagc cacctcagga ggagcagggc attatcactc attgcaagac 
     4801 ccattccatg gtgtttacca ttcacatcct ggtaatccaa gtaatgttac accagcagat 
     4861 agctgtcatt gcagccggac tccagatgca tttatttcaa gtttcgctca ccatgcttca 
     4921 tgtcatttta gtagaagtaa tgtgccagta gagacaactg atgaaatacc atttagtttc 
     4981 tctgacaggc tcagaatttc tgaaaaatag tgtccaaccc agctttc 
//

