LOCUS       pGEX-6P-3-hSC35(AA1-115)        5329 bp    DNA     circular UNA  1-AUG-2017
DEFINITION  LMBP 9170.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9170
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11876424|
COMMENT     VNTDBDATE|-11876424|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGEX-6P-3-hSC35(AA1-115)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
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COMMENT                                   "@N" 1 11.0743 -10.454 0 -10 3.74348 1.16519 
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FEATURES             Location/Qualifiers
     promoter        184..212
                     /vntifkey="30"
                     /label=tac_promoter
                     /note="Unknown feature type:Promoter"
     CDS             258..944
                     /vntifkey="4"
                     /label=GST
     Misc._feature   918..938
                     /vntifkey="21"
                     /label=precision
                     /note="Unknown feature type:Misc._feature"
     CDS             951..1298
                     /vntifkey="4"
                     /label=hSC35(1-115)
     Misc._feature   complement(1379..1401)
                     /vntifkey="21"
                     /label=pGEX_3_primer
                     /note="Unknown feature type:Misc._feature"
     CDS             1737..2597
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 2"
     gene            1737..2597
                     /gene="Ampicillin"
                     /vntifkey="60"
                     /note="Unknown feature type:Gene"
     rep_origin      2752..3371
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             3669..4760
                     /vntifkey="4"
                     /label=lacI
     promoter        4809..4838
                     /vntifkey="30"
                     /label=lac_promoter
                     /note="Unknown feature type:Promoter"
     Misc._feature   4900..5055
                     /vntifkey="21"
                     /label=lacZ_a
                     /note="Unknown feature type:Misc._feature"
BASE COUNT     1288 a      1330 c      1410 g      1301 t 
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atgagctacg 
      961 gccgcccccc tcccgatgtg gagggtatga cctccctcaa ggtggacaac ctgacctacc 
     1021 gcacctcgcc cgacacgctg aggcgcgtct tcgagaagta cgggcgcgtc ggcgacgtgt 
     1081 acatcccgcg ggaccgctac accaaggagt cccgcggctt cgccttcgtt cgctttcacg 
     1141 acaagcgcga cgctgaggac gctatggatg ccatggacgg ggccgtgctg gacggccgcg 
     1201 agctgcgggt gcaaatggcg cgctacggcc gccccccgga ctcacaccac agccgccggg 
     1261 gaccgccacc ccgcaggtac gggggcggtg gctactaaga attcccgggt cgactcgagc 
     1321 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 
     1381 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 
     1441 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 
     1501 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc 
     1561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 
     1621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     1681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     1741 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     1801 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     1861 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     1921 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 
     1981 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     2041 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     2101 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     2161 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     2221 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     2281 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     2341 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     2401 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     2461 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     2521 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     2581 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     2641 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     2701 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     2761 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     2821 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     2881 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     2941 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     3001 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     3061 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     3121 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     3181 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     3241 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     3301 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     3361 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     3421 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     3481 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     3541 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg 
     3601 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 
     3661 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 
     3721 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 
     3781 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 
     3841 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 
     3901 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 
     3961 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 
     4021 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 
     4081 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 
     4141 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 
     4201 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 
     4261 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 
     4321 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 
     4381 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 
     4441 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 
     4501 ataccgaaga cagctcatgt tatatcccgc cgtcaaccac catcaaacag gattttcgcc 
     4561 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 
     4621 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 
     4681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 
     4741 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 
     4801 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 
     4861 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca 
     4921 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 
     4981 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 
     5041 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag 
     5101 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat 
     5161 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc 
     5221 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag 
     5281 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt 
//
