LOCUS       pEF6-P2A-Zeo            4544 bp    DNA     circular UNA  4-APR-2014
DEFINITION  LMBP 9214.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|694346904|
COMMENT     VNTNAME|pEF6-P2A-Zeo|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-P2A-Zeo" 0 4544 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 4906 4915 1 4544 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #1=(CFSignal (CObList) "intron hEF1a promoter" 21 0 0 582 1520 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 21 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #3=(CFSignal (CObList) "P2A" 4 0 0 1615 1680 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1615..1680") 
COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 21 0 0 2228 2302 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3803..3877") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 21 0 1 2228 2302 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3803..3877)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 21 0 1 2616 3007 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "AMP" 4 0 1 3548 4408 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "ZeoR" 4 0 0 1696 2070 0 (CStringList) 
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COMMENT                         "DNA Plasmid 'pEF6-P2A-Zeo'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9214." 
COMMENT                         1) 
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COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                         "Created: 04/04/14 10:08" 1) 
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COMMENT                         "Last Modified: 04/04/14 10:08" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4544 bp" 1) 
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COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 14.2029 -12.1948 40.3368 40.3368 0.01 10 -1 4544 4544 1 0 0) 
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COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
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COMMENT               #136=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -889 100) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.95387 4.04499 1 0.082322) 0.8 1.8 0) 
COMMENT                             #139=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "P2A" "@N" 1 
COMMENT                                   10.5015 -8.71165 0 -10 1.76018 1.16519 #138#) 
COMMENT                             #140=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.187754 1.3764 1 0.082322) 0.8 1.8 1) 
COMMENT                             #141=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AMP" "@N" 1 
COMMENT                                   -10.0046 8.71165 0 -10 1.90893 1.16519 #140#) 
COMMENT                             #142=(CWideArrow 
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COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #75# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.41546 3.93317 1 0.082322) 0.8 1.8 0) 
COMMENT                             #143=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ZeoR" "@N" 1 
COMMENT                                   7.8808 -10.454 0 -10 2.256 1.16519 #142#)) 
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COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   4.16234 5.74168) 
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COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hEF1a promoter" 
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COMMENT                                   "intron hEF1a promoter" "@N" 1 16.7453 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 4.57607 -12.1963 0 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 4.37816 -13.9386 0 
COMMENT                                   -10 6.54489 1.16519 #153#) 
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COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 2.12189 
COMMENT                                   2.66307) 
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COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     Promoter        386..1529
                     /vntifkey="21"
                     /label=hEF1a\promoter
                     /note="Unknown feature type:Promoter"
     Intron          582..1520
                     /vntifkey="21"
                     /label=intron\hEF1a\promoter
                     /note="Unknown feature type:Intron"
     Promoter        1546..1562
                     /vntifkey="21"
                     /label=T7\promoter
                     /note="Unknown feature type:Promoter"
     CDS             1615..1680
                     /vntifkey="4"
                     /label=P2A
     Terminator      2228..2302
                     /vntifkey="21"
                     /label=SV40\polyA\early
                     /note="Unknown feature type:Terminator"
     Terminator      complement(2228..2302)
                     /vntifkey="21"
                     /label=SV40\polyA\late
                     /note="Unknown feature type:Terminator"
     Replication_ori complement(2616..3007)
                     /vntifkey="21"
                     /label=pMB1\ori
                     /note="Unknown feature type:Replication_ori"
     CDS             complement(3548..4408)
                     /vntifkey="4"
                     /label=AMP
     CDS             1696..2070
                     /vntifkey="4"
                     /label=ZeoR
BASE COUNT      987 a      1163 c      1276 g      1118 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtggaagc 
     1621 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 
     1681 cacagtggcg gccgcatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac 
     1741 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag 
     1801 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac 
     1861 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac 
     1921 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc 
     1981 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc 
     2041 gtgcacttcg tggccgagga gcaggactga gcacttcgtg gccgaggagc aggactgaca 
     2101 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     2161 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     2221 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     2281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     2341 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 
     2401 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     2461 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     2521 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     2581 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     2641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     2701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagga 
     2761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     2821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     2881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     2941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     3061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     3121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     3181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     3241 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     3301 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     3361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     3421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     3481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     3541 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     3601 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     3661 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     3721 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     3781 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     3841 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     3901 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     3961 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4021 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4081 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     4141 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     4201 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4261 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4321 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     4381 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     4441 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     4501 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//
