LOCUS       pEF6-P2A-Hyg            5204 bp    DNA     circular UNA  4-APR-2014
DEFINITION  LMBP 9215.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|694349250|
COMMENT     VNTNAME|pEF6-P2A-Hyg|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-P2A-Hyg" 0 5204 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5204 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "hEF1a promoter" 21 0 0 386 1529 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #1=(CFSignal (CObList) "intron hEF1a promoter" 21 0 0 582 1520 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 21 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #3=(CFSignal (CObList) "P2A" 4 0 0 1615 1680 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1615..1680") 
COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 21 0 0 2888 2962 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4463..4537") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 21 0 1 2888 2962 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4463..4537)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 21 0 1 3276 3667 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4851..5242)") 
COMMENT        #7=(CFSignal (CObList) "AMP" 4 0 1 4208 5068 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(5783..6643)") 
COMMENT        #8=(CFSignal (CObList) "HygR" 4 0 0 1696 2730 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1696..2730")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #9=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT            (CObjectList 
COMMENT             #10=(CRefLinePar 
COMMENT                  (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pEF6-P2A-Hyg" 2) 5 
COMMENT                  "" 0 4) 
COMMENT             #11=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "DNA Plasmid 'pEF6-P2A-Hyg'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9215." 
COMMENT                         1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Created: 04/04/14 10:47" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Last Modified: 04/04/14 10:47" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 5204 bp" 1) 
COMMENT                    #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
COMMENT                    #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "form: Circular" 1))) "General Description") 
COMMENT             #20=(CFolderPar 
COMMENT                  (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                   (CObjectList 
COMMENT                    #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Division: UNA" 1) 
COMMENT                    #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 4-APR-2014" 1))) 
COMMENT                  "Standard Fields") 
COMMENT             #24=(CRefLinePar 
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COMMENT                  0 0) 
COMMENT             #25=(CFolderPar 
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COMMENT                  "Annotations") 
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COMMENT                                       "Start: 1615  End: 1680" 1) 
COMMENT                                  #30=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   1615..1680" 1))) "P2A") 
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COMMENT                                 (CObjectList 
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COMMENT                                       "Start: 1696  End: 2730" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #35=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   1696..2730" 1))) "HygR") 
COMMENT                           #36=(CFolderPar 
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COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(5783..6643)" 1))) "AMP"))) 
COMMENT                         "CDS (3 total)") 
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COMMENT                                 (CObjectList 
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COMMENT                                       "Start: 386   End: 1529" 1) 
COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Unknown feature type:Promoter" 1) 
COMMENT                                  #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   386..1529" 1))) "hEF1a promoter") 
COMMENT                           #46=(CFolderPar 
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COMMENT                                       "Start: 582   End: 1520" 1) 
COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Unknown feature type:Intron" 1) 
COMMENT                                  #49=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #50=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   582..1520" 1))) 
COMMENT                                "intron hEF1a promoter") 
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COMMENT                                       "Start: 1546  End: 1562" 1) 
COMMENT                                  #53=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Unknown feature type:Promoter" 1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #55=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   1546..1562" 1))) "T7 promoter") 
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COMMENT                                       "Start: 2888  End: 2962" 1) 
COMMENT                                  #58=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Unknown feature type:Terminator" 1) 
COMMENT                                  #59=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                "SV40 polyA late") 
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COMMENT                                       "Unknown feature type:Replication_ori" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   complement(4851..5242)" 1))) 
COMMENT                                "pMB1 ori"))) "Misc. Feature (6 total)"))) 
COMMENT                  "Feature Map") 
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COMMENT                         "   Similarity between ambiguous nucleotides (if any)" 
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COMMENT                    #73=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "is calculated as an AVERAGE similarity" 1))) 
COMMENT                  "Motifs (both strands, best fit only)")))) 
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COMMENT                                   "intron hEF1a promoter" "@N" 1 16.2271 
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COMMENT                                   "SV40 polyA early" "@N" 1 -8.88009 -12.1963 0 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     Promoter        386..1529
                     /vntifkey="21"
                     /label=hEF1a\promoter
                     /note="Unknown feature type:Promoter"
     Intron          582..1520
                     /vntifkey="21"
                     /label=intron\hEF1a\promoter
                     /note="Unknown feature type:Intron"
     Promoter        1546..1562
                     /vntifkey="21"
                     /label=T7\promoter
                     /note="Unknown feature type:Promoter"
     CDS             1615..1680
                     /vntifkey="4"
                     /label=P2A
     Terminator      2888..2962
                     /vntifkey="21"
                     /label=SV40\polyA\early
                     /note="Unknown feature type:Terminator"
     Terminator      complement(2888..2962)
                     /vntifkey="21"
                     /label=SV40\polyA\late
                     /note="Unknown feature type:Terminator"
     Replication_ori complement(3276..3667)
                     /vntifkey="21"
                     /label=pMB1\ori
                     /note="Unknown feature type:Replication_ori"
     CDS             complement(4208..5068)
                     /vntifkey="4"
                     /label=AMP
     CDS             1696..2730
                     /vntifkey="4"
                     /label=HygR
BASE COUNT     1147 a      1329 c      1450 g      1278 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtggaagc 
     1621 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 
     1681 cacagtggcg gccgcatgga tagatccgga aagcctgaac tcaccgcgac gtctgtcgag 
     1741 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa 
     1801 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc 
     1861 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc 
     1921 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc 
     1981 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg 
     2041 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg 
     2101 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc 
     2161 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg 
     2221 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg 
     2281 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca 
     2341 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc 
     2401 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg 
     2461 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac 
     2521 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga 
     2581 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga 
     2641 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc 
     2701 cccagcactc gtccgagggc aaaggaatag gcacttcgtg gccgaggagc aggactgaca 
     2761 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 
     2821 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 
     2881 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     2941 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     3001 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 
     3061 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     3121 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     3181 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     3241 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     3301 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     3361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagga 
     3421 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3481 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3541 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3601 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3661 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     3721 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     3781 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     3841 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     3901 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     3961 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4021 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4081 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4141 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     4801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     4861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     5101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//
