LOCUS       pEF6-P2A-Blast          4568 bp    DNA     circular UNA 24-APR-2014
DEFINITION  LMBP 9216.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|696085113|
COMMENT     VNTNAME|pEF6-P2A-Blast|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-P2A-Blast" 0 4568 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5558 5557 1 4568 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "hEF1a promoter" 21 0 0 386 1529 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #1=(CFSignal (CObList) "intron hEF1a promoter" 21 0 0 582 1520 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 21 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #3=(CFSignal (CObList) "P2A" 4 0 0 1615 1680 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1615..1680") 
COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 21 0 0 2252 2326 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3827..3901") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 21 0 1 2252 2326 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3827..3901)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 21 0 1 2640 3031 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4215..4606)") 
COMMENT        #7=(CFSignal (CObList) "AMP" 4 0 1 3572 4432 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(5147..6007)") 
COMMENT        #8=(CFSignal (CObList) "BlastR" 4 0 0 1696 2094 0 (CStringList) 
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COMMENT                         "DNA 'pEF6-P2A-Blast'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "DMB 3964  (Invitrogen vector)." 1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Created: 04/24/14 12:58" 1) 
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COMMENT                         "Last Modified: 04/24/14 12:58" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4568 bp" 1) 
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COMMENT                         "Division: UNA" 1) 
COMMENT                    #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 24-APR-2014" 1))) 
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COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
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COMMENT         -3261) 1 1 0 1 1 
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COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "P2A" "@N" 1 
COMMENT                                   10.5944 -8.71165 0 -10 1.76018 1.16519 #138#) 
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COMMENT                             #141=(CLabel 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AMP" "@N" 1 
COMMENT                                   -9.96959 8.71165 0 -10 1.90893 1.16519 #140#) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "BlastR" "@N" 1 
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COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "hEF1a promoter" 
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COMMENT                                   "SV40 polyA early" "@N" 1 -4.76155 -13.9386 0 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 -4.33222 -12.1963 0 
COMMENT                                   -10 6.54489 1.16519 #153#) 
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COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     Promoter        386..1529
                     /vntifkey="21"
                     /label=hEF1a\promoter
                     /note="Unknown feature type:Promoter"
     Intron          582..1520
                     /vntifkey="21"
                     /label=intron\hEF1a\promoter
                     /note="Unknown feature type:Intron"
     Promoter        1546..1562
                     /vntifkey="21"
                     /label=T7\promoter
                     /note="Unknown feature type:Promoter"
     CDS             1615..1680
                     /vntifkey="4"
                     /label=P2A
     Terminator      2252..2326
                     /vntifkey="21"
                     /label=SV40\polyA\early
                     /note="Unknown feature type:Terminator"
     Terminator      complement(2252..2326)
                     /vntifkey="21"
                     /label=SV40\polyA\late
                     /note="Unknown feature type:Terminator"
     Replication_ori complement(2640..3031)
                     /vntifkey="21"
                     /label=pMB1\ori
                     /note="Unknown feature type:Replication_ori"
     CDS             complement(3572..4432)
                     /vntifkey="4"
                     /label=AMP
     CDS             1696..2094
                     /vntifkey="4"
                     /label=BlastR
BASE COUNT     1024 a      1150 c      1246 g      1148 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtggaagc 
     1621 ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct 
     1681 cacagtggcg gccgcatggc caagcctttg tctcaagaag aatccaccct cattgaaaga 
     1741 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct 
     1801 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct 
     1861 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt 
     1921 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag 
     1981 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg 
     2041 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt 
     2101 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta 
     2161 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     2221 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 
     2281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     2341 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 
     2401 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     2461 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     2521 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     2581 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     2641 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     2701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     2761 gaacatgtga gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     3241 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     4561 ctgacgtc 
//
