LOCUS       pUCmini                 1758 bp    DNA     circular      5-SEP-2018
DEFINITION  LMBP 9221.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9221
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-SEP-2018
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|699982267|
COMMENT     VNTDBDATE|-12452721|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pUCmini|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "NdeI" "NruI" "PstI" "PvuI" "SalI" "XbaI") (CStringList "atg" "gtg") 
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COMMENT        #2=(CRSite (CStringList) "DraI" "tttaaa" 4 0 3 774 0 793 0 1485 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 37 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HpaI" "gttaac" 4 0 1 25 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 2 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 34 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 29 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 17 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 9 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 53 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 1278 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 55 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 61 0 0 "")) 
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COMMENT                          "DNA Plasmid 'pUCmini'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 06/05/14 03:31" 1) 
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COMMENT                          "length: 1758 bp" 1) 
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COMMENT                                   (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Dra}I (1485)" "@N (@S)" 5 -12.4707 
COMMENT                                   6.96932 0 -10 4.8722 1.05854 #224#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #226=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1734439494 100) 
COMMENT                     (CObjectList 
COMMENT                      #227=(CLabel (CWidget 0 (15 #16# 0) 1 2 0 0 #136# 0 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 108 41 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 9221" 
COMMENT                            "LMBP 9221" 65535 0.0555433 -2.88825 0 -10 6.91678 
COMMENT                            1.49356 Nil) 
COMMENT                      #228=(CLabel 
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COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 05-SEP-2018" 
COMMENT                            "Last modified: 05-SEP-2018" 65535 -0.185144 
COMMENT                            -4.44347 0 -10 11.789 1.05854 Nil)) (CObjectList)) 
COMMENT               #229=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1952935525 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 25084368 
COMMENT       (CStringList) 17563701 25075936 
COMMENT       (CObList 
COMMENT        #230=(CSampFrag "pUCmini" 967 61 "XbaI" 1028 "BglI" (LOGPEN 0 0 0) 
COMMENT              3.06261e-316 5.13627e-291) 
COMMENT        #231=(CSampFrag "pUCmini" 541 1278 "PvuI" 61 "XbaI" (LOGPEN 0 0 0) 
COMMENT              3.06261e-316 2.5779e-312) 
COMMENT        #232=(CSampFrag "pUCmini" 250 1028 "BglI" 1278 "PvuI" (LOGPEN 0 0 0) 
COMMENT              3.06262e-316 2.5779e-312))))
FEATURES             Location/Qualifiers
     CDS             complement(834..1694)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(72..293)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT      435 a       448 c       418 g       457 t 
ORIGIN
        1 acgcgtcgcg aggccatatg ggttaaccca tggccaagct tgcatgcctg caggtcgact 
       61 ctagaggatc cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
      121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
      181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
      241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
      301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
      361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
      421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
      481 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
      541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
      601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
      661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
      721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
      781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
      841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
      901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
      961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     1021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     1081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     1141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     1201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     1261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     1321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     1381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     1441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     1501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     1561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     1621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     1681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     1741 ctcatgagcg gatacata 
//
