LOCUS       pDEST-myr              7207 bp    DNA     circular     21-JUN-2013
DEFINITION  LMBP 9234.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     CDS             complement(6211..7071)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3910..4704
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      4880..4954
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4880..4954)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5268..5670)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5093..5176)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3743..3818
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3072..3500
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      2802..3026
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        3505..3786
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     attenuator      complement(1058..1182)
                     /vntifkey="0"
                     /label=attR1
     attenuator      2638..2762
                     /vntifkey="0"
                     /label=attR2
     CDS             1291..1950
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2292..2597
                     /vntifkey="4"
                     /label=ccdB
     promoter        1190..1242
                     /vntifkey="30"
                     /label=lacUV5\promoter
BASE COUNT     1753 a      1773 c      1812 g      1869 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagctga 
     1081 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca 
     1141 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc 
     1201 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg 
     1261 gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 
     1321 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 
     1381 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 
     1441 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 
     1501 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 
     1561 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 
     1621 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 
     1681 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 
     1741 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 
     1801 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 
     1861 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 
     1921 tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga 
     1981 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat 
     2041 acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca 
     2101 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac 
     2161 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga 
     2221 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag 
     2281 ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 
     2341 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg 
     2401 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 
     2461 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 
     2521 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 
     2581 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat 
     2641 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa 
     2701 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg 
     2761 gtgatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 
     2821 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 
     2881 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 
     2941 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 
     3001 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 
     3061 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     3121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     3181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     3241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     3301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     3361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     3421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     3481 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 
     3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 
     3601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     3661 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 
     3721 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 
     3781 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 
     3841 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 
     3901 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 
     3961 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 
     4021 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 
     4081 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 
     4141 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 
     4201 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 
     4261 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 
     4321 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 
     4381 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 
     4441 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 
     4501 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 
     4561 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 
     4621 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 
     4681 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc 
     4741 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 
     4801 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 
     4861 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 
     4921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
     4981 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 
     5041 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     5101 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 
     5161 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 
     5221 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 
     5281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
     5341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
     5401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
     5461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
     5521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     5581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     5641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     5701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     5761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     5821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     5881 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     5941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     6001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     6061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     6121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     6181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     6241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     6301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     6361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     6421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     6481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     6541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     6601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     6661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     6721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     6781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     6841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     6901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     6961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     7021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     7081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     7141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     7201 tgacgtc 
//

