LOCUS       pC2HB-GC               10922 bp    DNA     circular UNA 19-MAR-1980
DEFINITION  LMBP 9242.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pC2HB-GC|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|


FEATURES             Location/Qualifiers
     CDS             321..2503
                     /vntifkey="4"
                     /label=CmLEU2
     misc_feature    2741..3854
                     /vntifkey="21"
                     /label=Integration\sequence
     CDS             4499..5402
                     /vntifkey="4"
                     /label=kanamycin\resistance
     promoter        5563..7001
                     /vntifkey="29"
                     /label=MET3p
     misc_feature    7008..7043
                     /vntifkey="21"
                     /label=SV40\NLS
     CDS             7044..7661
                     /vntifkey="4"
                     /label=LexA
     glycine_linker  7668..7685
                     /vntifkey="21"
                     /label=glycine\linker
                     /note="Unknown feature type:glycine_linker"
     misc_marker     7686..7712
                     /vntifkey="22"
                     /label=HA\tag
     attenuator      7743..7867
                     /vntifkey="0"
                     /label=attR1
     CDS             7976..8635
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             8977..9282
                     /vntifkey="4"
                     /label=ccdB
     attenuator      9323..9447
                     /vntifkey="0"
                     /label=attR2
     terminator      9478..9991
                     /vntifkey="43"
                     /label=CaACT1term
     rep_origin      10121..10794
                     /vntifkey="33"
                     /label=pucORi
BASE COUNT     3162 a      2193 c      2376 g      3191 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg 
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga 
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc 
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa 
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc 
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc 
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc 
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc 
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga 
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa 
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc 
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa 
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata 
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt 
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta 
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca 
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac 
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac 
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg 
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt 
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga 
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt 
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg 
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac 
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta 
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc 
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg 
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc 
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg 
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta 
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga 
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg 
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta 
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta 
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat 
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat 
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg 
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta 
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac 
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa 
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac 
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca 
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca 
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat 
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt 
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg 
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa 
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc 
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg 
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag 
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga 
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc 
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt 
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa 
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga 
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg 
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac 
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca 
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt 
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga 
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca 
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt 
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga 
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc 
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg 
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga 
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat 
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca 
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc 
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt 
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat 
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg 
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct 
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga 
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg 
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa 
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa 
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact 
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg 
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caattgtggt 
     6481 gtttttctaa caaatttttc aacatttaaa gtatttctgg aacttttcta caaggaacga 
     6541 catagatcca ttaatgcgcc cctctaaaac aatacccata atgtcttctt aagtatgcat 
     6601 ctgcttataa atgtttctgg atttacttta gttagaatgt cgacacgaca atgaactatt 
     6661 gctgtagatc acgtgcgatc actgaattat gagtttcttt ttatttttct ttttgggctt 
     6721 ttttttttgc aatttttttt ttccaccttt gccaattgcg tagtcacata taatgtatac 
     6781 aatttttgca actctgacac tataattgat ccaataataa agtatataat agatcatcaa 
     6841 gtatacgtaa tctccccaaa aaaaaaattg cataaaataa aatctataaa ccttttttag 
     6901 atttttagtt gttccttttt tctttctatt cttcaacttc caagtgttgt cactttcttc 
     6961 ctgttaacta tatttaaaag taaataccct ccccagaaaa ctccggaatg gctgctcctc 
     7021 caaaaaagaa gagaaaggta gctagagaat taacaaaacg acaaagcgaa atatataact 
     7081 atattaaaca agttgttcaa atgaaaggtt atccgcctag tgttcgcgaa attggtgagg 
     7141 cagttggctt agcatccagt tcaactgttc atggtcacct ttcacgtctt gaagaaaaag 
     7201 gctatataag aagagatcca acgaaaccac gtgctataga aattgtaagt gatcaaacaa 
     7261 atgataatat taatatggaa gaaacgattc atgtgccagt tattggtaaa gtcacagcag 
     7321 gtgttcctat taccgcagta gaaaatattg aagaatattt tccattacct gaacacttaa 
     7381 catcgacaca caatagcgac atattcatat taaacgtcgt aggcgacagt atgattgagg 
     7441 ctggtatatt agacggagac aaagtaattg ttcgcagtca aaccatagca gaaaatggag 
     7501 acattattgt tgctatgact gaggaagatg aagcaactgt caaacgcttc tataaagaaa 
     7561 aaaatcgtta tcgattacaa cctgaaaata gtacaatgga gccaatttac ctcgacaatg 
     7621 ttgctgtaat cgggaaagta attggtttgt accgcgaaat gagatctggt gctggtggtg 
     7681 ttggttatcc gtatgatgtg cctgactacg caggtgctgg tggtgttggt gctagcagga 
     7741 tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat 
     7801 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 
     7861 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa 
     7921 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga 
     7981 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 
     8041 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 
     8101 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 
     8161 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 
     8221 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 
     8281 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 
     8341 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 
     8401 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 
     8461 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 
     8521 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 
     8581 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg 
     8641 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg 
     8701 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag 
     8761 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg 
     8821 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg 
     8881 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga 
     8941 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat 
     9001 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc 
     9061 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt 
     9121 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg 
     9181 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat 
     9241 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata 
     9301 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt 
     9361 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc 
     9421 tcgttcagct ttcttgtaca aagtggtgat cgcgtagtac ttaaccgcgc cgtcgacgag 
     9481 tgaaattctg gaaatctgga aatctggttt tgtattcttg ttattcttct ttttgttatt 
     9541 acatatataa cttgttactt ttttaaaaaa atctttgttt attttataaa tatataaaac 
     9601 taaatttaag aaaaagagaa aaatgtttta tttgagagat tgaaatttta cttgaattta 
     9661 gcttagcttt tataaagtat tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt 
     9721 aagactataa aatagccacc caaggcattt ctatatcttg ttgttgttgt tttcatcttc 
     9781 tgtatcagag gaacttattt tattattttc gtcacgggta ttttctcttg tttgatgatt 
     9841 catcccattc attccatcat aaaattctag aaggaccacc tttgattgta aatagtaata 
     9901 attaccaccc ttatttaatt atttatttaa cttatttatt tatttattat acatatatac 
     9961 aaatctaata aagtgaaaat ctcccccttc acacgtggcc gaggagcagg actgacacgt 
    10021 gctaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
    10081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
    10141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
    10201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 
    10261 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 
    10321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 
    10381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 
    10441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 
    10501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 
    10561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 
    10621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 
    10681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 
    10741 aaacgccagc aacgcggcct ttttacggtt cctgggcttt tgctggcctt ttgctcacat 
    10801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 
    10861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 
    10921 ag 
//
