LOCUS       pEF6-F-hCARMA2-H171N        8891 bp    DNA     circular      6-APR-2018
DEFINITION  LMBP 9328.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12233397|
COMMENT     VNTDBDATE|-12233397|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEF6-F-hCARMA2-H171N|
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FEATURES             Location/Qualifiers
     CDS             complement(3137..3997)
                     /vntifkey="4"
                     /label=Ampicilin\resistance
     promoter        complement(4032..4116)
                     /vntifkey="30"
                     /label=bla
     rep_origin      complement(2330..2991)
                     /vntifkey="33"
                     /label=pUC\ori
     polyA_site      1817..1947
                     /vntifkey="26"
                     /label=SV40\polyA
     CDS             1261..1659
                     /vntifkey="4"
                     /label=Blasticidin\resistance
     promoter        1194..1260
                     /vntifkey="29"
                     /label=EM-7
     promoter        831..1139
                     /vntifkey="29"
                     /label=SV40+ori
     rep_origin      376..804
                     /vntifkey="33"
                     /label=f1\ori
     polyA_site      106..330
                     /vntifkey="26"
                     /label=BGH\polyA
     primer_bind     100..117
                     /vntifkey="28"
                     /label=BGH\reverse
     misc_feature    64..117
                     /vntifkey="21"
                     /label=His6
     CDS             15..44
                     /vntifkey="4"
                     /label=Myc
     primer_bind     5679..5698
                     /vntifkey="28"
                     /label=T7
     promoter        4519..5678
                     /vntifkey="29"
                     /label=pEF1-alpha
     CDS             5850..8864
                     /vntifkey="4"
                     /label=hCARD14\mutant
     misc_marker     5790..5813
                     /vntifkey="22"
                     /label=FLAG\epitope
     mutation        6324..6326
                     /vntifkey="62"
                     /label=H171N
                     /note="WT: CAC"
BASE COUNT     1952 a      2394 c      2577 g      1968 t 
ORIGIN
        1 ctagagggcc cttcgaacaa aaactcatct cagaagagga tctgaatatg cataccggtc 
       61 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt 
      121 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 
      181 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 
      241 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 
      301 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 
      361 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
      421 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
      481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
      541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
      601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
      661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
      721 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
      781 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 
      841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
      901 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
      961 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     1021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     1081 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     1141 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac 
     1201 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc 
     1261 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc 
     1321 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc 
     1381 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg 
     1441 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga 
     1501 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg 
     1561 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt 
     1621 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca 
     1681 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 
     1741 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 
     1801 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     1861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     1921 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 
     1981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     2041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 
     2101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 
     2161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     2221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     2281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     2341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     2401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     2461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     2521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     2581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     2641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     2701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     2761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     2821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     2881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     2941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3001 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     3061 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     3121 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     3181 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     3241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     3301 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     3361 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     3421 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     3481 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     3541 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     3601 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     3661 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     3721 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     3781 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     3841 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     3901 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     3961 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4021 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     4081 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 
     4141 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata 
     4201 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 
     4261 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 
     4321 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt 
     4381 gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct 
     4441 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca 
     4501 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
     4561 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
     4621 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
     4681 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
     4741 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 
     4801 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 
     4861 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 
     4921 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 
     4981 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 
     5041 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 
     5101 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 
     5161 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 
     5221 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 
     5281 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 
     5341 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 
     5401 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 
     5461 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 
     5521 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 
     5581 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 
     5641 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag 
     5701 acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca gtgtggtgga 
     5761 attctgcaga taaacttaag cttaccatgg attacaagga cgatgacgat aaggtaccta 
     5821 aggatccagt gtggtggaat tctgcagata tgggggaact gtgccgcagg gactccgcac 
     5881 tcacggcact ggacgaggag acactgtggg agatgatgga gagccaccgc cacaggatcg 
     5941 tacgctgcat ctgccccagc cgcctcaccc cctacctgcg ccaggccaag gtgctgtgcc 
     6001 agctggacga ggaggaggtg ctgcacagcc cccggctcac caacagcgcc atgcgggccg 
     6061 ggcacttgct ggatttgctg aagactcgag ggaagaacgg ggccatcgcc ttcctggaga 
     6121 gcctgaagtt ccacaaccct gacgtctaca ccctggtcac cgggctgcag cctgatgttg 
     6181 acttcagtaa ctttagcggt ctcatggaga catccaagct gaccgagtgc ctggctgggg 
     6241 ccatcggcag cctgcaggag gagctgaacc aggaaaaggg gcagaaggag gtgctgctgc 
     6301 ggcggtgcca gcagctgcag gagaacctgg gcctggccga gacccgtgcc gagggcctgc 
     6361 accagctgga ggctgaccac agccgcatga agcgtgaggt tagcgcacac ttccatgagg 
     6421 tgctgaggct gaaggacgag atgctcagcc tctcgctgca ctatagcaat gcgctgcagg 
     6481 agaaggagct ggccgcctca cgctgccgca gcctgcagga ggagctgtat ctactgaagc 
     6541 aggagctgca gcgagccaac atggtttcct cctgtgagct ggaattgcaa gagcagtccc 
     6601 tgaggacagc cagcgaccag gagtccgggg atgaggagct gaaccgcctg aaggaggaga 
     6661 atgagaaact gcgctcgctg actttcagcc tggcggagaa ggacattctg gagcagagcc 
     6721 tggacgaggc gcgggggagc cgacaggagc tggtggagcg catccactcg ctgcgggagc 
     6781 gggccgtggc tgccgagagg cagcgagagc agtactggga agagaaggaa cagaccctgc 
     6841 tgcagttcca gaagagtaag atggcctgcc aactctacag ggagaaggtg aatgcgctgc 
     6901 aggcccaggt gtgcgagctg cagaaggagc gagaccaggc gtactccgcg agggacagtg 
     6961 ctcagaggga gatttcccag agcctggtgg agaaggactc cctccgcagg caggtgttcg 
     7021 agctgacgga ccaggtctgc gagctgcgca cacagcttcg ccagctgcag gcagagcctc 
     7081 cgggtgtgct caagcaggaa gccaggacca gggagccctg tccacgggag aagcagcggc 
     7141 tggtgcggat gcatgccatc tgccccagag acgacagcga ctgcagcctc gtcagctcca 
     7201 cagagtctca gctcttgtcg gacctgagtg ccacgtccag ccgcgagctg gtggacagct 
     7261 tccgctccag cagccccgcg ccccccagcc agcagtccct gtacaagcgg gtggccgagg 
     7321 acttcgggga agaaccctgg tctttcagca gctgcctgga gatcccggag ggagacccgg 
     7381 gagccctgcc gggagctaag gcaggcgacc cacacctgga ttatgagctc ctagacacgg 
     7441 cagaccttcc gcagctggaa agcagcctgc agccagtctc ccctggaagg cttgatgtct 
     7501 cggagagcgg cgtcctcatg cggcggaggc cagcccgcag gatcctgagc caggtcacca 
     7561 tgctggcgtt ccagggggat gcattgctgg agcagatcag cgtcatcggc gggaacctca 
     7621 cgggcatctt catccaccgg gtcaccccgg gctcggcggc ggaccagatg gccttgcgcc 
     7681 cgggcaccca gattgtgatg gttgattacg aagcctcaga gcccttgttc aaggcagtcc 
     7741 tggaggacac gaccctggag gaggccgtgg ggcttctcag gagggtggac ggcttctgct 
     7801 gcctgtctgt gaaggtcaac acggacggtt ataagaggct actccaggac ctggaggcca 
     7861 aagtggcgac ctcgggggac tcattctaca tccgggtcaa cctggccatg gagggcaggg 
     7921 ccaaagggga gctgcaggtg cattgcaacg aggtcctgca cgtcaccgac accatgttcc 
     7981 agggctgcgg ctgctggcat gcccaccgcg tgaactctta caccatgaag gatactgccg 
     8041 cgcacggcac catccccaac tactccaggg ctcagcagca gctcatagcc ctcatccagg 
     8101 acatgactca gcagtgcacc gtgacccgca agccatcttc tgggggacca cagaagctgg 
     8161 tccgcatcgt cagtatggac aaagccaagg ccagccctct gcgtttgtcc tttgacaggg 
     8221 gccagttgga ccccagcagg atggagggct ccagcacgtg cttctgggcc gagagctgcc 
     8281 tcaccctggt gccctatacc ctggtgcggc cccatcgacc cgcccggccc cggcctgtgc 
     8341 tcctcgtgcc cagggcggtt gggaagatcc tgagcgagaa actgtgcctc ctccaagggt 
     8401 ttaagaagtg cctggcagag tacttgagcc aggaggagta tgaggcctgg agccagagag 
     8461 gggacatcat ccaggaggga gaggtgtccg ggggccgctg ctgggtgacc cgccatgctg 
     8521 tggagtccct catggaaaag aacacccatg ccctcctgga cgtccagctg gacagtgtct 
     8581 gcaccctgca caggatggac atcttcccca tcgtcatcca cgtctctgtc aacgagaaga 
     8641 tggcaaagaa gctcaagaag ggcctacagc ggttgggcac ctcagaggag cagctcctgg 
     8701 aggctgcgag gcaggaggag ggagacctgg accgggcgcc ctgtctatac agcagcctgg 
     8761 ctcctgacgg ctggagcgac ctggacggcc tgctcagctg tgtccgccag gccatcgccg 
     8821 acgagcagaa gaaggtggtg tggacggagc agagcccccg atgaatccag cacagtggcg 
     8881 gccgctcgag t 
//
