LOCUS       pT2L-UNO                4106 bp    DNA     circular     25-NOV-2014
DEFINITION  LMBP 9386.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 9386.
            Last modified: 25-NOV-2014
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|706979911|
COMMENT     VNTDBDATE|715516746|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2L-UNO|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Pery Hackett|
COMMENT     VNTOAUTHOREML|perry@biosci.cbs.umn.edu|
COMMENT     VNTOAUTHORWWW|http://www.cbs.umn.edu/labs/perry|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pT2L-UNO" 0 4106 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "HincII" "NotI" "PvuI" "PvuII" "EcoRI" "EcoRV" "ScaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4106 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "HincII" "gtyrac" 4 0 3 199 0 349 0 1331 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 975 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 422 0 3011 0 4056 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 653 0 1572 0 4085 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 992 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1004 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 3121 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "IR/DR(R) Rmut13,d130,143,150" 4 0 0 1079 1306 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1079..1306") 
COMMENT        #8=(CFSignal (CObList) "IR/DR(L) Lmut44" 4 0 1 119 345 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(119..345)") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter" 30 0 0 74 94 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "74..94") 
COMMENT        #10=(CFSignal (CObList) "T3 promoter" 30 0 1 1366 1385 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1366..1385)") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2570 3427 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2570..3427)") 
COMMENT        #12=(CFSignal (CObList) "f1 ori" 33 0 0 3559 4014 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3558..4014") 
COMMENT        #13=(CFSignal (CObList) "hEF1-HTLV promoter (pUNO)" 29 0 0 411 963 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "411..963") 
COMMENT        #14=(CFSignal (CObList) "lac promoter" 30 0 1 1449 1532 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 1624 2026 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #16=(CFSignal (CObList) "Last modified: 25-NOV-2014" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #17=(CFSignal (CObList) "Collection N\260.: 9386" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pT2L-UNO'" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Collection N\260.: 9386." 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Pery Hackett" 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/22/14 03:18" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/25/14 10:39" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4106 bp" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 22-AUG-2014" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Pery Hackett" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:perry@biosci.cbs.umn.edu\">perry@biosci.cbs.umn.edu</a>" 
COMMENT                          1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "WWW: <a href=\"http://www.cbs.umn.edu/labs/perry\">http://www.cbs.umn.edu/labs/perry</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "" 1))) "Last modified: 25-NOV-2014") 
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COMMENT                                 "" 1))) "Collection N\260.: 9386"))) 
COMMENT                   "Annotations") 
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COMMENT                                        "Start: 119   End: 345  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]" 
COMMENT                                        1) 
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COMMENT                                        "   complement(119..345)" 1))) 
COMMENT                                 "IR/DR(L) Lmut44") 
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COMMENT                                        "Start: 1079  End: 1306" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (dT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" 
COMMENT                                        1) 
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COMMENT                                        "   1079..1306" 1))) 
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COMMENT                                        "   complement(2570..3427)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                   #62=(CLinePar 
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COMMENT                                        "   411..963" 1))) 
COMMENT                                 "hEF1-HTLV promoter (pUNO)"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                   #67=(CLinePar 
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COMMENT                                        "   74..94" 1))) "T7 promoter") 
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COMMENT                                        "Start: 1366  End: 1385 (Complementary)" 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                        "   3558..4014" 1))) "f1 ori"))) 
COMMENT                          "Replication Origin (2 total)"))) "Feature Map") 
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COMMENT                                        " N1: 975 " 1))))) "NotI: 1 site") 2 6 
COMMENT                          "GCGGCCGC" "CGCCGGCG") 
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COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                          "AGTACT" "TCATGA") 
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COMMENT        #239=(CSampFrag "pT2L-UNO" 2513 1572 "PvuII" 4085 "PvuII" (LOGPEN 0 0 0) 
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FEATURES             Location/Qualifiers
     CDS             1079..1306
                     /vntifkey="4"
                     /label=IR/DR(R)\Rmut13,d130,143,150
                     /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (dT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]"
     CDS             complement(119..345)
                     /vntifkey="4"
                     /label=IR/DR(L)\Lmut44
                     /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]"
     promoter        74..94
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(1366..1385)
                     /vntifkey="30"
                     /label=T3\promoter
     CDS             complement(2570..3427)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      3559..4014
                     /vntifkey="33"
                     /label=f1\ori
     promoter        411..963
                     /vntifkey="29"
                     /label=hEF1-HTLV\promoter\(pUNO)
     promoter        complement(1449..1532)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1624..2026)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT      999 a      1024 c      1001 g      1082 t 
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca 
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta 
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt 
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat 
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt 
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagctaga ggatctgcga 
      421 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 
      481 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 
      541 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 
      601 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 
      661 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 
      721 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 
      781 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 
      841 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 
      901 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacctgaga 
      961 tcaccatcta gagcggccgc gcgcactagt gaattccatg gatatcaagc ttgtggaagg 
     1021 ctactcgaaa tgtttgaccc aagttaaaca atttaaaggc aatgctacca aatactaatt 
     1081 gagtgtatgt aaacttctga cccactggga atgtgatgaa agaaataaaa gctgaaatga 
     1141 atcattctct ctactattat tctgatattt cacattctta aaataaagtg gtgatcctaa 
     1201 ctgacctaag acagggaatt tttactagga ttaaatgtca ggaattgtga aaaagtgagt 
     1261 ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtata gggatcctct 
     1321 agctagagtc gacctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt 
     1381 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     1441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 
     1501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     1561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     1621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     1681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     1741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     1801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     1861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     1921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     1981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     2041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     2101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     2161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     2221 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     2281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     2341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     2401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     2461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     2521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     2581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     2641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     2701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     2761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     2821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     2881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     2941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     3001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     3061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     3121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     3181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     3241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     3301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     3361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     3421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     3481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     3541 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 
     3601 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 
     3661 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 
     3721 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 
     3781 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 
     3841 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 
     3901 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 
     3961 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc 
     4021 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 
     4081 ccagctggcg aaagggggat gtgctg 
//
