LOCUS       pT2-SVT                 6623 bp    DNA     circular      9-SEP-2014
DEFINITION  LMBP 9411.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|708614561|
COMMENT     VNTDBDATE|708614441|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2-SVT|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #5=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 3102 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 953 0 1479 0 2648 0 
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COMMENT        #17=(CFSignal (CObList) "SV40L" 30 0 0 2747 3042 1 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "SV40E" 30 0 1 2710 2991 1 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "TATA" 23 0 0 3405 3408 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "759..762") 
COMMENT        #21=(CFSignal (CObList) "F1 Ori" 33 0 0 5656 6112 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3010..3466") 
COMMENT        #22=(CFSignal (CObList) "Amp" 4 0 1 4668 5525 1 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "ColE Ori" 33 0 0 3724 4604 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1078..1958") 
COMMENT        #24=(CFSignal (CObList) "LacZ promoter" 30 0 1 3508 3630 1 (CStringList) 
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COMMENT        #25=(CFSignal (CObList) "T3" 30 0 1 3464 3483 1 (CStringList) 
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COMMENT        #26=(CFSignal (CObList) "T7" 30 0 0 6278 6298 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "74..94") 
COMMENT        #27=(CFSignal (CObList) "IR/DR(L) Lmut44" 4 0 1 6323 6549 1 
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COMMENT                                        "SV40 small t- antigen" 1) 
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COMMENT                                        "IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (\306T), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" 
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FEATURES             Location/Qualifiers
     terminator      70..166
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     terminator      complement(70..166)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     CDS             complement(2115..2639)
                     /vntifkey="4"
                     /label=t
                     /note="SV40 small t- antigen"
     misc_feature    complement(167..2047)
                     /vntifkey="21"
                     /label=T
                     /note="2nd exon of the SV40 large T-antigen"
     intron          complement(2048..2393)
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 large T-antigen"
     misc_feature    complement(2394..2639)
                     /vntifkey="21"
                     /label=T
                     /note="1st exon of the SV40 large T-antigen"
     rep_origin      complement(2678..2753)
                     /vntifkey="33"
                     /label=SVORI
                     /note="bidirectional origin of SV40"
     promoter        2747..3042
                     /vntifkey="30"
                     /label=SV40L
                     /note="SV40 late promoter"
     promoter        complement(2710..2991)
                     /vntifkey="30"
                     /label=SV40E
                     /note="SV40 early promoter"
     modified_base   6319..6322
                     /vntifkey="23"
                     /label=TATA
     modified_base   3405..3408
                     /vntifkey="23"
                     /label=TATA
     rep_origin      5656..6112
                     /vntifkey="33"
                     /label=F1\Ori
     CDS             complement(4668..5525)
                     /vntifkey="4"
                     /label=Amp
     rep_origin      3724..4604
                     /vntifkey="33"
                     /label=ColE\Ori
     promoter        complement(3508..3630)
                     /vntifkey="30"
                     /label=LacZ\promoter
     promoter        complement(3464..3483)
                     /vntifkey="30"
                     /label=T3
     promoter        6278..6298
                     /vntifkey="30"
                     /label=T7
     CDS             complement(6323..6549)
                     /vntifkey="4"
                     /label=IR/DR(L)\Lmut44
                     /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]"
     CDS             3177..3404
                     /vntifkey="4"
                     /label=IR/DR(R)\Rmut13,Æ130,143,150
                     /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ÆT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]"
BASE COUNT     1802 a      1537 c      1356 g      1928 t 
ORIGIN
        1 ctagagccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 
       61 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 
      121 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 
      181 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct 
      241 gattatgatc atgaacagac tgtgaggact gaggggcctg aaatgagcct tgggactgtg 
      301 aatcaatgcc tgtttcatgc cctgagtctt ccatgttctt ctccccacca tcttcatttt 
      361 tatcagcatt ttcctggctg tcttcatcat catcatcact gtttcttagc caatctaaaa 
      421 ctccaattcc catagccaca ttaaacttca ttttttgata cactgacaaa ctaaactctt 
      481 tgtccaatct ctctttccac tccacaattc tgctctgaat actttgagca aactcagcca 
      541 caggtctgta ccaaattaac ataagaagca aagcaatgcc actttgaatt attctctttt 
      601 ctaacaaaaa ctcactgcgt tccaggcaat gctttaaata atctttgggc ctaaaatcta 
      661 tttgttttac aaatctggcc tgcagtgttt taggcacact gtactcattc atggtgacta 
      721 ttccaggggg aaatatttga gttcttttat ttaggtgttt cttttctaag tttaccttaa 
      781 cactgccatc caaataatcc cttaaattgt ccaggttatt aattccctga cctgaaggca 
      841 aatctctgga ctcccctcca gtgcccttta catcctcaaa aactactaaa aactggtcaa 
      901 tagctactcc tagctcaaag ttcagcctgt ccaagggcaa attaacattt aaagctttcc 
      961 ccccacataa ttcaagcaaa gcagctgcta atgtagtttt accactatca attggtcctt 
     1021 taaacagcca gtatcttttt ttaggaatgt tgtacaccat gcattttaaa aagtcataca 
     1081 ccactgaatc cattttgggc aacaaacagt gtagccaagc aactccagcc atccattctt 
     1141 ctatgtcagc agagcctgta gaaccaaaca ttatatccat cctatccaaa agatcattaa 
     1201 atctgtttgt taacatttgt tctctagtta attgtaggct atcaacccgc tttttagcta 
     1261 aaacagtatc aacagcctgt tggcatatgg ttttttggtt tttgctgtca gcaaatatag 
     1321 cagcatttgc ataatgcttt tcatggtact tatagtggct gggctgttct tttttaatac 
     1381 attttaaaca catttcaaaa ctgtactgaa attccaagta catcccaagc aataacaaca 
     1441 catcatcaca ttttgtttcc attgcatact ctgttacaag cttccaggac acttgtttag 
     1501 tttcctctgc ttcttctgga ttaaaatcat gctcctttaa cccacctggc aaactttcct 
     1561 caataacaga aaatggatct ctagtcaagg cactatacat caaatattcc ttattaaccc 
     1621 ctttacaaat taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca 
     1681 ctctatgcct gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac 
     1741 ttataaaggt tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct 
     1801 ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa 
     1861 aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag 
     1921 gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc 
     1981 aaaacaggtt ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg 
     2041 ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa 
     2101 attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac 
     2161 cacagaagta aggttccttc acaaagatca agtccaaacc acattctaaa gcaatcgaag 
     2221 cagtagcaat caacccacac aagtggatct ttcctgtata attttctatt ttcatgcttc 
     2281 atcctcagta agcacagcaa gcatatgcag ttagcagaca ttttctttgc acactcaggc 
     2341 cattgtttgc agtacattgc atcaacacca ggatttaagg aagaagcaaa tacctcagtt 
     2401 gcatcccaga agcctccaaa gtcaggttga tgagcatatt ttactccatc ttccattttc 
     2461 ttgtacagag tattcatttt cttcattttt tcttcatctc ctcctttatc aggatgaaac 
     2521 tccttgcatt tttttaaata tgcctttctc atcagaggaa tattccccca ggcactcctt 
     2581 tcaagaccta gaaggtccat tagctgcaaa gattcctctc tgtttaaaac tttatccatc 
     2641 tttgcaaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa 
     2701 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg 
     2761 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg 
     2821 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc 
     2881 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc 
     2941 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gctggttctt 
     3001 tccgcctcag aaggtaccta accaagttcc tctttcagag gttatttcag gccatggtgc 
     3061 tgcgccgatg ataagctgtc aaacatgaga attccatgga tatcaagctt gtggaaggct 
     3121 actcgaaatg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa tactaattga 
     3181 gtgtatgtaa acttctgacc cactgggaat gtgatgaaag aaataaaagc tgaaatgaat 
     3241 cattctctct actattattc tgatatttca cattcttaaa ataaagtggt gatcctaact 
     3301 gacctaagac agggaatttt tactaggatt aaatgtcagg aattgtgaaa aagtgagttt 
     3361 aaatgtattt ggctaaggtg tatgtaaact tccgacttca actgtatagg gatcctctag 
     3421 ctagagtcga cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta 
     3481 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     3541 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     3601 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     3661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     3721 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     3781 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     3841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     3901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     3961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     4021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     4081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     4141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     4201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     4261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     4321 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     4381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     4501 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     4561 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     4621 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     4681 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     4741 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     4801 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     4861 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     4921 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     4981 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     5041 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     5101 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     5161 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     5221 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     5281 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     5341 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     5401 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     5461 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     5521 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     5581 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     5641 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     5701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     5761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     5821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     5881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     5941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     6001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     6061 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc 
     6121 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 
     6181 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 
     6241 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg 
     6301 aattggagct cggatcccta tacagttgaa gtcggaagtt tacatacact taagttggag 
     6361 tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca atagttttgg 
     6421 caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa caattgttta 
     6481 cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc agaagtttac 
     6541 atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat gatgtcatgg 
     6601 ctttagaagc tagatctggc cat 
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