LOCUS       pENTR-L4R1-EF1a            3790 bp    DNA     circular      6-AUG-2013
DEFINITION  LMBP 9471.
SOURCE      
  ORGANISM  
COMMENT     
            
            
COMMENT     VNTDATE|669653740|
COMMENT     VNTNAME|pENTR-L4R1-EF1a|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pENTR-L4R1-EF1a" 0 3790 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "hCMV-IE enhancer" 
COMMENT             "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT             "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 0 
COMMENT             (CStringList "euk. promoter") 0) 
COMMENT         #1=(COligo "hCMV-IE promoter and enhancer" 
COMMENT             "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT             "Fragment of pCDM8; Length: 584" 0 (CStringList "euk. promoter") 0)) 
COMMENT        1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 3790 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "kanamycin resistance" 4 0 0 2178 2993 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1583..2398") 
COMMENT        #3=(CFSignal (CObList) "pMB1 ori" 33 0 0 3510 122 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2915..122") 
COMMENT        #4=(CFSignal (CObList) "attL4" 0 0 0 593 688 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "593..688") 
COMMENT        #5=(CFSignal (CObList) "T7" 30 0 1 2034 2050 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1439..1455)") 
COMMENT        #6=(CFSignal (CObList) "T1 terminator" 43 0 0 427 470 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "427..470") 
COMMENT        #7=(CFSignal (CObList) "attR1" 0 0 1 1835 1959 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1273..1397)") 
COMMENT        #8=(CFSignal (CObList) "EF1a" 29 0 0 691 1834 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) 
COMMENT       (CObList 
COMMENT        #9=(CMSite "hCMV-IE enhancer" 
COMMENT            "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT            "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 0) 
COMMENT        #10=(CMSite "hCMV-IE promoter and enhancer" 
COMMENT             "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT             "Fragment of pCDM8; Length: 584" 0)) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #11=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #12=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pENTR-L4R1-EF1a" 2) 
COMMENT                   5 "" 0 4) 
COMMENT              #13=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pENTR-L4R1-EF1a'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/06/13 02:55" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/06/13 02:55" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3790 bp" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-AUG-2013" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #24=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #25=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #28=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 593 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #29=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 593   End: 688 " 1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   593..688" 1))) "attL4") 
COMMENT                            #32=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1835 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1835  End: 1959 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #34=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1273..1397)" 1))) "attR1"))) 
COMMENT                          "Attenuator (2 total)") 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2178 (3 #2# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2178  End: 2993" 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #40=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1583..2398" 1))) 
COMMENT                                 "kanamycin resistance"))) "CDS (1 total)") 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 691 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 691   End: 1834" 1))) "EF1a"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #44=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2034 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2034  End: 2050 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1439..1455)" 1))) "T7"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #49=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #50=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3510 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3510  End: 122 " 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2915..122" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)") 
COMMENT                     #54=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #55=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 427 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 427   End: 470 " 1) 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   427..470" 1))) "T1 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
COMMENT              #59=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 14 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "   Similarity between ambiguous nucleotides (if any)" 
COMMENT                          1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "is calculated as an AVERAGE similarity" 1) 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 35118760 (9 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CGroupPar (CParagraph 0 (11 #9# 0) 1 2 2 0 180) 
COMMENT                                 (CObjectList 
COMMENT                                  #64=(COlyLinePar 
COMMENT                                       (CLinePar 
COMMENT                                        (CParagraph 0 
COMMENT                                         (17 
COMMENT                                          #65=(COligo "hCMV-IE enhancer" 
COMMENT                                               "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT                                               "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 
COMMENT                                               0 (CStringList) 0) 0) 1 2 2 0 181) 
COMMENT                                        " GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCA" 
COMMENT                                        1)) 
COMMENT                                  #66=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                       "Fragment of pCAGGS-FLAGAVPShOMI; Length: 378" 
COMMENT                                       1))))) "hCMV-IE enhancer (0 sites)") 
COMMENT                     #67=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 24465920 (9 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #68=(CGroupPar 
COMMENT                                 (CParagraph 0 (11 #10# 0) 1 2 2 0 180) 
COMMENT                                 (CObjectList 
COMMENT                                  #69=(COlyLinePar 
COMMENT                                       (CLinePar 
COMMENT                                        (CParagraph 0 
COMMENT                                         (17 
COMMENT                                          #70=(COligo 
COMMENT                                               "hCMV-IE promoter and enhancer" 
COMMENT                                               "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT                                               "Fragment of pCDM8; Length: 584" 
COMMENT                                               0 (CStringList) 0) 0) 1 2 2 0 181) 
COMMENT                                        " GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT                                        1)) 
COMMENT                                  #71=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                       "Fragment of pCDM8; Length: 584" 1))))) 
COMMENT                          "hCMV-IE promoter and enhancer (0 sites)"))) 
COMMENT                   "Motifs (both strands)")))) 
COMMENT       (CGraphView 
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COMMENT          #73=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
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COMMENT          #80=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #81=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #82=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #83=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 
COMMENT               2 "?" 0) 
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COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT               "@N (@H%)" 0) 
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COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #87=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
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COMMENT          #94=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #95=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #96=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #97=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #98=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #99=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 
COMMENT               3 "@T @N " 0) 
COMMENT          #100=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #101=(CWidgetStyle "Annotation" 0 0 1 
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COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #103=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
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COMMENT          #104=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #105=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #106=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
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FEATURES             Location/Qualifiers
     CDS             2178..2993
                     /vntifkey="4"
                     /label=kanamycin\resistance
     rep_origin      3510..122
                     /vntifkey="33"
                     /label=pMB1\ori
     attenuator      593..688
                     /vntifkey="0"
                     /label=attL4
     promoter        complement(2034..2050)
                     /vntifkey="30"
                     /label=T7
     terminator      427..470
                     /vntifkey="43"
                     /label=T1\terminator
     attenuator      complement(1835..1959)
                     /vntifkey="0"
                     /label=attR1
     promoter        691..1834
                     /vntifkey="29"
                     /label=EF1a
BASE COUNT      874 a       916 c      1025 g       975 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 
      661 ataatgccaa ctttgtatag aaaagttgga gcccacagtc cccgagaagt tggggggagg 
      721 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 
      781 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 
      841 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg 
      901 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc 
      961 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag 
     1021 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg 
     1081 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga 
     1141 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga 
     1201 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg 
     1261 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg 
     1321 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct 
     1381 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc 
     1441 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg 
     1501 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc 
     1561 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt 
     1621 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt 
     1681 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc 
     1741 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt 
     1801 tcaaagtttt tttcttccat ttcaggtgtc gtgaacaagt ttgtacaaaa aagctgaacg 
     1861 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
     1921 tacataatac tgtaaaacac aacatatcca gtcactatga accaactact tagatggtat 
     1981 tagtgacctg tagaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg 
     2041 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct 
     2101 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 
     2161 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat 
     2221 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 
     2281 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga 
     2341 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 
     2401 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 
     2461 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg 
     2521 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc 
     2581 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 
     2641 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 
     2701 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca 
     2761 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 
     2821 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 
     2881 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 
     2941 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat 
     3001 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct 
     3061 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag 
     3121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     3181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     3241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     3301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     3361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     3421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     3481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa 
     3541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     3601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     3661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     3721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     3781 ctcacatgtt 
//
