LOCUS       pEXPR-E-sciN             5960 bp    DNA     circular     30-SEP-2011
DEFINITION  LMBP 9476.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9476
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-SEP-2011
            
            
            
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|598099854|
COMMENT     VNTDBDATE|602593678|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-E-sciN|
COMMENT     VNTAUTHORNAME|GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CObList) -1 "") 
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COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 682 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 403 0 692 0 2283 0 3018 0 
COMMENT            0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1030 0 1668 0 2692 0 
COMMENT            3761 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 2120 0 5429 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 611 0 1405 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 1363 0 0 "")) 
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COMMENT        #9=(CFSignal (CObList) "FLAG epitope" 22 0 0 696 719 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "696..719") 
COMMENT        #10=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 27 610 0 
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COMMENT        #12=(CFSignal (CObList) "SP6 promoter" 30 0 1 1379 1395 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "BGH polyA" 43 0 0 1421 1645 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1585..1809") 
COMMENT        #14=(CFSignal (CObList) "f1 ori" 33 0 0 1691 2119 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1855..2283") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 2290 2365 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2454..2529") 
COMMENT        #16=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 2457 3251 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2621..3415") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3425 3499 0 
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COMMENT        #18=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3425 3499 0 
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COMMENT        #19=(CFSignal (CObList) "lac promoter" 30 0 1 3638 3721 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 3813 4215 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "SV40 early promoter" 29 0 0 2124 2333 0 
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COMMENT        #23=(CFSignal (CObList) "attB2.1" 0 0 1 1337 1359 0 (CStringList) 
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COMMENT                          "Foreign object. Author: GeneCorner Plasmid Collection. Original author: UNKNOWN" 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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FEATURES             Location/Qualifiers
     CDS             complement(4756..5616)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     696..719
                     /vntifkey="22"
                     /label=FLAG\epitope
     promoter        27..610
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     promoter        655..671
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(1379..1395)
                     /vntifkey="30"
                     /label=SP6\promoter
     terminator      1421..1645
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      1691..2119
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      2290..2365
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             2457..3251
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3425..3499
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3425..3499)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(3638..3721)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(3813..4215)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        2124..2333
                     /vntifkey="29"
                     /label=SV40\early\promoter
     attenuator      730..752
                     /vntifkey="0"
                     /label=attB1.1
     attenuator      complement(1337..1359)
                     /vntifkey="0"
                     /label=attB2.1
     CDS             756..794
                     /vntifkey="4"
                     /label=E-tag
     CDS             804..1336
                     /vntifkey="4"
                     /label=SciN
BASE COUNT     1393 a      1549 c      1528 g      1490 t 
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 
      661 gactcactat agggagaccc aagcttacca ccatggacta caaggatgac gatgacaaag 
      721 cggccatcac aagtttgtac aaaaaagttg gcatgggtgc gccggtgccg tatccggacc 
      781 cgctggaacc gcgtgcggcc gctaatgaat ggcgtaaccc cactcggtgg ttatgtgcgg 
      841 tagctatgcc ttttgcactg ctcctgcttt ccggatgcgg cagtagcgat gcgttacctg 
      901 acctcgaatc acagcgactc gacctgagcg tgaaagcctc cgataaggtg aatcctgaca 
      961 atcagaagaa ggccgcgccc attgagatac gtgtttatga actgaaaaat gacgccgctt 
     1021 tcacgacagc tgattactgg tcgctccatg acaacgacaa atccgtcctt accgacgatt 
     1081 tagtgcgtcg cgacagcttt attttgcgtc ccggcgaaga gaaaaaactg cgtcgcccgc 
     1141 tgaatgcgca gaccacggca atcggcgtac tggccggata ccgtaacctg gccaaatcgg 
     1201 tctggcgggt aacctacaaa atcccggaag ccccggaaaa agcctggtac agcagcttca 
     1261 tatcggggaa aggaaaagtg cagttggagg cggaactgga acaaagcgcc attgtaatta 
     1321 cggaacggga taaatgccca actttcttgt acaaagtggg atctagaggg ccctattcta 
     1381 tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag 
     1441 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 
     1501 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 
     1561 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 
     1621 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 
     1681 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     1741 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     1801 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg 
     1861 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     1921 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     1981 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     2041 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     2101 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 
     2161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     2221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     2281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     2341 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     2401 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 
     2461 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 
     2521 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 
     2581 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 
     2641 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 
     2701 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 
     2761 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 
     2821 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 
     2881 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 
     2941 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 
     3001 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 
     3061 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 
     3121 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 
     3181 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 
     3241 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 
     3301 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     3361 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     3421 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     3481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     3541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     3601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     3661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     3721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     3781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     3841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     3901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     3961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     4021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     4081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     4141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 
     4201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     4261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     4321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     4381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     4441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     4501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     4561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     4621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     4681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     4741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     4801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     4861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     4921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     4981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     5041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     5101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     5161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     5221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     5281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     5341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     5401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     5461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     5521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     5581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     5641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     5701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc 
     5761 gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga tgccgcatag 
     5821 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa 
     5881 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt 
     5941 aggcgttttg cgctgcttcg 
//

