LOCUS pCfB255 6050 bp DNA circular 1-APR-2015 DEFINITION LMBP 9494. SOURCE ORGANISM COMMENT Accession number: LMBP 9494 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 1-APR-2015 COMMENT VNTDATE|728837741| COMMENT VNTNAME|pCfB255| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pCfB255" 0 6050 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) (CStringList "HindIII" "NcoI" "NsiI" "XmnI" "HaeII") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6050 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2315 0 0 "") COMMENT #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3760 0 0 "") COMMENT #2=(CRSite (CStringList) "NsiI" "atgcat" 6 0 2 2687 0 4627 0 0 "") COMMENT #3=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 672 0 0 "") COMMENT #4=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 125 0 1110 0 1567 0 2529 COMMENT 0 4920 0 5525 0 0 "")) COMMENT (CObList COMMENT #5=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1951 2052 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "complement(1951..2052)") COMMENT #6=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2110 2300 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "2110..2300") COMMENT #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 5156 5555 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(5156..5555)") COMMENT #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6049 859 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(6049..859)") COMMENT #9=(CFSignal (CObList) "loxP" 21 0 1 2348 2381 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2348..2381)") COMMENT #10=(CFSignal (CObList) "loxP" 21 0 1 3834 3867 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3834..3867)") COMMENT #11=(CFSignal (CObList) "K. lactis URA3 promoter" 29 0 1 3319 3814 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(3319..3814)") COMMENT #12=(CFSignal (CObList) "K. lactis URA3 terminator" 43 0 1 2397 2514 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2397..2514)") COMMENT #13=(CFSignal (CObList) "T7 promoter" 30 0 1 3913 3929 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3913..3929)") COMMENT #14=(CFSignal (CObList) "T1 terminator" 43 0 1 1163 1200 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(1163..1200)") COMMENT #15=(CFSignal (CObList) "FRT" 21 0 0 1244 1277 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1244..1277") COMMENT #16=(CFSignal (CObList) "FRT" 21 0 1 4548 4581 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4548..4581)") COMMENT #17=(CFSignal (CObList) "T7 promoter" 30 0 1 4582 4596 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4582..4596)") COMMENT #18=(CFSignal (CObList) COMMENT "S. cerevisiae chrom. X integration targeting sequence" 21 0 0 4016 COMMENT 4506 0 (CStringList) (CStringList) 1 1 1 1 "4016..4505") COMMENT #19=(CFSignal (CObList) COMMENT "S. cerevisiae chrom. X integration targeting sequence" 21 0 0 1318 COMMENT 1848 0 (CStringList) (CStringList) 1 1 1 1 "1318..1848") COMMENT #20=(CFSignal (CObList) "USER cloning cassette" 21 0 0 2082 2110 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2087..2110") COMMENT #21=(CFSignal (CObList) "K. lactis URA3" 4 0 1 2515 3318 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList COMMENT #22=(CFSignal (CObList) "LMBP 9494" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #23=(CFSignal (CObList) "Last modified: 1-APR-2015" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #25=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pCfB255" 2) 5 "" COMMENT 0 4) COMMENT #26=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid 'pCfB255'" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9494." COMMENT 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 03/19/15 02:55" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 04/01/15 11:36" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 6050 bp" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 26-MAR-2015" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: URA3" 1))) "Standard Fields") COMMENT #39=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #40=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #42=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) COMMENT "" 1))) "LMBP 9494") COMMENT #43=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) COMMENT (CObjectList COMMENT #44=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) COMMENT "" 1))) "Last modified: 1-APR-2015"))) COMMENT "Annotations") COMMENT #45=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #46=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #47=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2515 (3 #21# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2515 End: 3318 (Complementary)" COMMENT 1))) "K. lactis URA3") COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 6049 (3 #8# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 6049 End: 859 (Complementary)" COMMENT 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(6049..859)" 1))) COMMENT "ampicillin resistance"))) "CDS (2 total)") COMMENT #53=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #54=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1244 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1244 End: 1277" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1244..1277" 1))) "FRT") COMMENT #58=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1318 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1318 End: 1848" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1318..1848" 1))) COMMENT "S. cerevisiae chrom. X integration targeting sequence") COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2082 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2082 End: 2110" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2087..2110" 1))) COMMENT "USER cloning cassette") COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2348 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2348 End: 2381 (Complementary)" COMMENT 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2348..2381)" 1))) "loxP") COMMENT #70=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3834 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3834 End: 3867 (Complementary)" COMMENT 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3834..3867)" 1))) "loxP") COMMENT #74=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4016 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4016 End: 4506" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4016..4505" 1))) COMMENT "S. cerevisiae chrom. X integration targeting sequence") COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4548 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4548 End: 4581 (Complementary)" COMMENT 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4548..4581)" 1))) "FRT"))) COMMENT "Misc. Feature (7 total)") COMMENT #82=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #83=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3319 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3319 End: 3814 (Complementary)" COMMENT 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3319..3814)" 1))) COMMENT "K. lactis URA3 promoter"))) COMMENT "Promoter Eukaryotic (1 total)") COMMENT #87=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #88=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3913 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3913 End: 3929 (Complementary)" COMMENT 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(3913..3929)" 1))) COMMENT "T7 promoter") COMMENT #92=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4582 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4582 End: 4596 (Complementary)" COMMENT 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(4582..4596)" 1))) COMMENT "T7 promoter"))) COMMENT "Promoter Prokaryotic (2 total)") COMMENT #96=(CFolderPar COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #97=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5156 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5156 End: 5555 (Complementary)" COMMENT 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(5156..5555)" 1))) COMMENT "pMB1 ori"))) "Replication Origin (1 total)") COMMENT #101=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #102=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1163 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1163 End: 1200 (Complementary)" COMMENT 1) COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #105=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1163..1200)" 1))) COMMENT "T1 terminator") COMMENT #106=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1951 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1951 End: 2052 (Complementary)" COMMENT 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1951..2052)" 1))) COMMENT "S. cerevisiae ADH1 terminator") COMMENT #110=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2110 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #111=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2110 End: 2300" 1) COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #113=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2110..2300" 1))) COMMENT "S. cerevisiae CYC1 terminator") COMMENT #114=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2397 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2397 End: 2514 (Complementary)" COMMENT 1) COMMENT #116=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2397..2514)" 1))) COMMENT "K. lactis URA3 terminator"))) COMMENT "Terminator (4 total)"))) "Feature Map") COMMENT #118=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #119=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #120=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 2315 " 1))))) COMMENT "HindIII: 1 site") 1 5 "AAGCTT" "TTCGAA") COMMENT #122=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #123=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #124=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 3760 " 1))))) "NcoI: 1 site") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #126=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 672 " 1))))) "XmnI: 1 site") 5 COMMENT 5 "GAANNNNTTC" "CTTNNNNAAG") COMMENT #128=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #129=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #130=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 2687 " 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (1 #2# 2) 1 2 2 0 191) COMMENT " N2: 4627 " 1))))) "NsiI: 2 sites") COMMENT 5 1 "ATGCAT" "TACGTA") COMMENT #132=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #133=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #134=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 125 " 1) COMMENT #135=(CLinePar COMMENT (CParagraph 0 (1 #4# 2) 1 2 2 0 191) COMMENT " N2: 1110 " 1) COMMENT #136=(CLinePar COMMENT (CParagraph 0 (1 #4# 3) 1 2 2 0 191) COMMENT " N3: 1567 " 1) COMMENT #137=(CLinePar COMMENT (CParagraph 0 (1 #4# 4) 1 2 2 0 191) COMMENT " N4: 2529 " 1) COMMENT #138=(CLinePar COMMENT (CParagraph 0 (1 #4# 5) 1 2 2 0 191) COMMENT " N5: 4920 " 1) COMMENT #139=(CLinePar COMMENT (CParagraph 0 (1 #4# 6) 1 2 2 0 191) COMMENT " N6: 5525 " 1))))) "HaeII: 6 sites") COMMENT 5 1 "RGCGCY" "YCGCGR"))) COMMENT "Restriction/Methylation Map") COMMENT #140=(CFolderPar COMMENT (CGroupPar (CParagraph 20 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #141=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) COMMENT " 2212: pCfB255: AvaI(5040) - AvaI(1202)" 1) 4 "" COMMENT 5040 1201) COMMENT #142=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) COMMENT " 1726: pCfB255: AvaI(1803) - AvaI(3529)" 1) 4 "" COMMENT 1803 3528) COMMENT #143=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) COMMENT " 1221: pCfB255: AvaI(3819) - AvaI(5040)" 1) 4 "" COMMENT 3819 5039) COMMENT #144=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) COMMENT " 601: pCfB255: AvaI(1202) - AvaI(1803)" 1) 4 "" COMMENT 1202 1802) COMMENT #145=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) COMMENT " 290: pCfB255: AvaI(3529) - AvaI(3819)" 1) 4 "" COMMENT 3529 3818))) "Restriction Fragments")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #146=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 5 "@N (@S)" 0) COMMENT #147=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #148=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 COMMENT "@L bp" 0) COMMENT #149=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) COMMENT #150=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 COMMENT 0 0) COMMENT #151=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 0 0 0) COMMENT #152=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) COMMENT 0 0 0) COMMENT #153=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #154=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #155=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) COMMENT #156=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 5 "@N (@S)" 0) COMMENT #157=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #158=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #159=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 COMMENT "@N (@H%)" 0) COMMENT #160=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #161=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #162=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #163=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #164=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) #165=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #166=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #167=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #168=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #169=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #170=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #171=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #172=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #173=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #174=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #175=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #176=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #177=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #178=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #179=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 COMMENT 65535 "@N" 0) COMMENT #180=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #181=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #182=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #183=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #184=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #185=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0) COMMENT #186=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #187=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #188=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #189=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 COMMENT (LOGBRUSH 0 10040064 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #190=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #191=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #192=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 COMMENT (LOGBRUSH 0 8388736 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #193=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #194=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #195=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #196=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16750848 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #197=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #198=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #199=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 COMMENT "@N" 0) COMMENT #200=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #201=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 COMMENT "@N (@H%)" 0) COMMENT #202=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 16711680 1 5 "@N (@S)" 0) COMMENT #203=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 32896 1 5 "@N (@S)" 0) COMMENT #204=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) COMMENT #205=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) COMMENT 0.164644 1.74233 0.164644 2.53336 COMMENT (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 COMMENT (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 COMMENT (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 COMMENT (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 COMMENT (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 COMMENT (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 COMMENT (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 COMMENT (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 COMMENT (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 COMMENT (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 COMMENT (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 COMMENT (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 COMMENT (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 COMMENT (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 COMMENT (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 COMMENT (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 COMMENT (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 COMMENT (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 COMMENT (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 COMMENT (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 COMMENT -3261) 1 1 0 1 1 COMMENT (mapper: 38.3357 -16.723 62.8334 62.8334 0.01 10 -1 6050 6050 1 0 0) COMMENT #206=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList COMMENT #207=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1305 100) COMMENT (CObjectList COMMENT #208=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #152# 25178068 0) COMMENT (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27522 COMMENT 6.27322 1 0.0214037) 0.103689) COMMENT #209=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #149# 28996212 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 99 37 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.91 0 "pCfB255" "@N" 1 0 0.871165 0 -10 COMMENT 5.33156 1.4801 Nil) COMMENT #210=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #148# 28994540 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 114 43 0 0 400 0 0 0 0 3 2 1 49 COMMENT "Courier New") 1.74233 1.05 0 "6050 bp" "@L bp" 16 COMMENT 0 -0.871165 0 -10.8 4.98143 1.75066 Nil)) COMMENT (CObjectList)) COMMENT #211=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList COMMENT #212=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -464 100) COMMENT (CObjectList COMMENT #213=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #150# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 5.3825 COMMENT 0.00207378 1 0.082322) 0.8 1.8 1) COMMENT #214=(CLabel (CWidget 0 (0 0) 1 2 0 0 #190# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "ampicillin resistance" "@N" 1 10.3817 COMMENT 13.6025 0 -10 9.05569 1.1618 #213#) COMMENT #215=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #150# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 COMMENT 2.83278 3.66644 1 0.082322) 0.8 1.8 1) COMMENT #216=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #190# 89926864 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "K. lactis URA3" COMMENT "@N" 1 -1.8848 -15.3938 0 -10 6.57294 1.1618 COMMENT #215#)) (CObjectList)) COMMENT #217=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -1167 100) COMMENT (CObjectList COMMENT #218=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #153# 1102 100) COMMENT (LOGPEN 0 10 16711680) 8 1 3.8396 1.9 COMMENT 0.082322 1) COMMENT #219=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 0 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT 10.4611 -12.3077 0 -10 2.05305 1.1618 #218#) COMMENT #220=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 1 2.29879 1.9 COMMENT 0.082322 1) COMMENT #221=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 29470636 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 COMMENT -11.596 -9.51574 0 -10 2.05305 1.1618 #220#) COMMENT #222=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 1 4.98433 1.9 COMMENT 0.082322 1) COMMENT #223=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 28706540 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 COMMENT 15.6975 4.54374 0 -10 2.11671 1.1618 #222#) COMMENT #224=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 1 1.55845 1.9 COMMENT 0.082322 1) COMMENT #225=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 0 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 COMMENT -15.0636 -0.541113 0 -10 2.11671 1.1618 #224#) COMMENT #226=(CLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 0.897917 1.60096 COMMENT 2.11007) COMMENT #227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# -1 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "S. cerevisiae chrom. X integration targeting sequence" COMMENT "@N" 1 -25.2575 -4.86927 0 -10 23.9204 1.1618 COMMENT #226#) COMMENT #228=(CLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #153# 544104808 COMMENT 100) (LOGPEN 0 10 16711680) 8 0.835356 COMMENT 4.35701 4.9076) COMMENT #229=(CLabel (CWidget 0 (0 0) 1 2 0 0 #191# 0 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "S. cerevisiae chrom. X integration targeting sequence" COMMENT "@N" 1 23.9959 -2.06462 0 -10 23.9204 1.1618 COMMENT #228#) COMMENT #230=(CScratch COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #153# 819 100) COMMENT (LOGPEN 0 10 16711680) 8 1 4.11542 1.9 COMMENT 0.082322 1) COMMENT #231=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #191# 393228 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "USER cloning cassette" "@N" 1 15.119 COMMENT -6.96932 0 -10 10.4403 1.1618 #230#)) COMMENT (CObjectList)) COMMENT #232=(CGroupWidget COMMENT (CWidget 29 (7 29 0) 1 2 0 0 Nil -956 100) COMMENT (CObjectList COMMENT #233=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #187# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) COMMENT 0.998308 2.31849 2.83278 1 0.082322) 0.8 1.8 COMMENT 1) COMMENT #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #190# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "K. lactis URA3 promoter" "@N" 1 -13.0999 COMMENT -12.3638 0 -10 10.9178 1.1618 #233#)) COMMENT (CObjectList)) COMMENT #235=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -816 100) COMMENT (CObjectList COMMENT #236=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #169# 0 100) COMMENT (LOGPEN 0 6 128) 6 0.835356 2.21687 2.30414) COMMENT 0.4 0.082322 0.915356 1.8 1.8 1) COMMENT #237=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #190# 90174072 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T7 promoter" COMMENT "@N" 1 -14.1129 -8.13945 0 -10 5.66578 1.1618 COMMENT #236#) COMMENT #238=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #169# 0 100) COMMENT (LOGPEN 0 6 128) 6 0.670712 1.52319 1.61046) COMMENT 0.4 0.082322 0.750712 1.8 1.8 1) COMMENT #239=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #190# 28542772 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T7 promoter" COMMENT "@N" 1 -17.1042 1.18605 0 -10 5.66578 1.1618 COMMENT #238#)) (CObjectList)) COMMENT #240=(CGroupWidget COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -1024 100) COMMENT (CObjectList COMMENT #241=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #162# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 COMMENT 0.513261 0.928017 1 0.082322) 0.8 1.8 1) COMMENT #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #190# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" COMMENT 1 -11.3796 10.8761 0 -10 4.12201 1.1618 #241#)) COMMENT (CObjectList)) COMMENT #243=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList COMMENT #244=(CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #153# 1081041522 COMMENT 100) (LOGPEN 0 10 16711680) 8 0.835356 COMMENT 4.14549 4.25125) COMMENT #245=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #200# 28861060 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "S. cerevisiae ADH1 terminator" "@N" 1 COMMENT 17.4557 -5.22699 0 -10 13.5756 1.1618 #244#) COMMENT #246=(CLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #153# 1699160122 COMMENT 100) (LOGPEN 0 10 16711680) 8 0.835356 COMMENT 3.88834 4.08638) COMMENT #247=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #200# 27877484 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "S. cerevisiae CYC1 terminator" "@N" 1 COMMENT 15.9556 -8.71165 0 -10 13.5756 1.1618 #246#) COMMENT #248=(CLine COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 0.835356 3.66644 COMMENT 3.7888) COMMENT #249=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #200# 1819241839 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "K. lactis URA3 terminator" "@N" 1 13.7712 COMMENT -13.6203 0 -10 11.2838 1.1618 #248#) COMMENT #250=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #153# 0 100) COMMENT (LOGPEN 0 10 16711680) 8 1 5.06832 1.9 COMMENT 0.082322 1) COMMENT #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #200# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 72 27 0 0 400 0 1 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T1 terminator" COMMENT "@N" 1 16.7997 5.79025 0 -10 6.03182 1.1618 COMMENT #250#)) (CObjectList))) (CObjectList)) COMMENT #252=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -254 100) COMMENT (CObjectList COMMENT #253=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -675 100) COMMENT (CObjectList COMMENT #254=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #205# 0 100) COMMENT (LOGPEN 0 0 16724736) 1 1 3.87382 1.9 COMMENT 0.082322 1) COMMENT #255=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #202# 28651364 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Hin}dIII (2315)" "@N (@S)" 5 12.4388 COMMENT -10.8837 0 -10 5.92042 1.06631 #254#)) COMMENT (CObjectList)) COMMENT #256=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -816 100) COMMENT (CObjectList COMMENT #257=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #205# 0 100) COMMENT (LOGPEN 0 0 16724736) 1 1 2.37552 1.9 COMMENT 0.082322 1) COMMENT #258=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #202# 89845048 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Nco}I (3760)" "@N (@S)" 5 -12.4237 COMMENT -10.9398 0 -10 4.87002 1.06631 #257#)) COMMENT (CObjectList)) COMMENT #259=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -886 100) COMMENT (CObjectList COMMENT #260=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #205# 0 100) COMMENT (LOGPEN 0 0 16724736) 1 1 5.57743 1.9 COMMENT 0.082322 1) COMMENT #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #202# 0 100) COMMENT (LOGPEN 0 0 16724787) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 16711680 COMMENT "{\\i Xmn}I (672)" "@N (@S)" 5 11.3279 COMMENT 11.9345 0 -10 4.59946 1.06631 #260#)) COMMENT (CObjectList)) COMMENT #262=(CGroupWidget COMMENT (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -1027 100) COMMENT (CObjectList COMMENT #263=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 3.4881 1.9 0.082322 1) COMMENT #264=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #203# 24902348 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nsi}I (2687)" "@N (@S)" 5 7.656 COMMENT -16.4684 0 -10 4.64721 1.06631 #263#) COMMENT #265=(CScratch COMMENT (CWidget 2 (1 #2# 2) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 1.47653 1.9 0.082322 1) COMMENT #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Nsi}I (4627)" "@N (@S)" 5 -16.3432 COMMENT 2.33119 0 -10 4.64721 1.06631 #265#)) COMMENT (CObjectList)) COMMENT #267=(CGroupWidget COMMENT (CWidget 6 (10 #4# 0) 1 2 0 0 Nil -675 100) COMMENT (CObjectList COMMENT #268=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 6.14461 1.9 0.082322 1) COMMENT #269=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #203# 2081718712 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (125)" "@N (@S)" 5 4.40219 COMMENT 15.0825 0 -10 4.75862 1.06631 #268#) COMMENT #270=(CScratch COMMENT (CWidget 2 (1 #4# 2) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 5.12327 1.9 0.082322 1) COMMENT #271=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (1110)" "@N (@S)" 5 16.3581 COMMENT 7.28466 0 -10 5.23607 1.06631 #270#) COMMENT #272=(CScratch COMMENT (CWidget 3 (1 #4# 3) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 4.64942 1.9 0.082322 1) COMMENT #273=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #203# 28535648 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (1567)" "@N (@S)" 5 16.3286 0 0 COMMENT -10 5.23607 1.06631 #272#) COMMENT #274=(CScratch COMMENT (CWidget 4 (1 #4# 4) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 3.65193 1.9 0.082322 1) COMMENT #275=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (2529)" "@N (@S)" 5 10.1582 COMMENT -15.2035 0 -10 5.23607 1.06631 #274#) COMMENT #276=(CScratch COMMENT (CWidget 5 (1 #4# 5) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 1.17272 1.9 0.082322 1) COMMENT #277=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #203# 27723296 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (4920)" "@N (@S)" 5 -13.6171 COMMENT 5.22699 0 -10 5.23607 1.06631 #276#) COMMENT #278=(CScratch COMMENT (CWidget 6 (1 #4# 6) 1 2 0 0 #204# 0 100) COMMENT (LOGPEN 0 0 39321) 1 1 0.545404 1.9 0.082322 COMMENT 1) COMMENT #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 3381657) 1 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.611111 32896 COMMENT "{\\i Hae}II (5525)" "@N (@S)" 5 -9.07332 COMMENT 12.7844 0 -10 5.23607 1.06631 #278#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #280=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -746 100) COMMENT (CObjectList COMMENT #281=(CLabel COMMENT (CWidget 0 (15 #22# 0) 1 2 0 0 #175# 14746048 100) COMMENT (LOGPEN 0 0 8388608) 1 COMMENT (LOGFONT 99 37 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.91 0 "LMBP 9494" COMMENT "LMBP 9494" 65535 0 -2.75331 0 -10 7.12997 1.54376 COMMENT Nil) COMMENT #282=(CLabel COMMENT (CWidget 0 (15 #23# 0) 1 2 0 0 #175# 393238 100) COMMENT (LOGPEN 24933976 29085416 16777216) 14746136 COMMENT (LOGFONT 66 25 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 1-APR-2015" COMMENT "Last modified: 1-APR-2015" 65535 0.334217 -4.29708 COMMENT 0 -10 10.5517 1.00265 Nil)) (CObjectList)) COMMENT #283=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -605 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 COMMENT (CObList COMMENT #284=(CSampFrag "pCfB255" 2212 5040 "AvaI" 1202 "AvaI" (LOGPEN 0 0 0) COMMENT 3.17257e-316 1.02894e-314) COMMENT #285=(CSampFrag "pCfB255" 1726 1803 "AvaI" 3529 "AvaI" (LOGPEN 0 0 0) COMMENT 1.6976e-313 2.32731e-300) COMMENT #286=(CSampFrag "pCfB255" 1221 3819 "AvaI" 5040 "AvaI" (LOGPEN 0 0 0) COMMENT 2.28051e+016 4.20657e+035) COMMENT #287=(CSampFrag "pCfB255" 601 1202 "AvaI" 1803 "AvaI" (LOGPEN 0 0 0) COMMENT 1.38017e-316 1.02894e-314) COMMENT #288=(CSampFrag "pCfB255" 290 3529 "AvaI" 3819 "AvaI" (LOGPEN 0 0 0) COMMENT 1.6976e-313 2.59903e-300)))) FEATURES Location/Qualifiers misc_feature 1318..1848 /vntifkey="21" /label=S.\cerevisiae\chrom.\X\integration\targeting\sequence terminator complement(1951..2052) /vntifkey="43" /label=S.\cerevisiae\ADH1\terminator terminator 2110..2300 /vntifkey="43" /label=S.\cerevisiae\CYC1\terminator misc_feature 4016..4506 /vntifkey="21" /label=S.\cerevisiae\chrom.\X\integration\targeting\sequence rep_origin complement(5156..5555) /vntifkey="33" /label=pMB1\ori CDS complement(6049..859) /vntifkey="4" /label=ampicillin\resistance misc_feature complement(2348..2381) /vntifkey="21" /label=loxP misc_feature complement(3834..3867) /vntifkey="21" /label=loxP promoter complement(3319..3814) /vntifkey="29" /label=K.\lactis\URA3\promoter terminator complement(2397..2514) /vntifkey="43" /label=K.\lactis\URA3\terminator promoter complement(3913..3929) /vntifkey="30" /label=T7\promoter terminator complement(1163..1200) /vntifkey="43" /label=T1\terminator misc_feature 1244..1277 /vntifkey="21" /label=FRT misc_feature complement(4548..4581) /vntifkey="21" /label=FRT promoter complement(4582..4596) /vntifkey="30" /label=T7\promoter misc_feature 2082..2110 /vntifkey="21" /label=USER\cloning\cassette CDS complement(2515..3318) /vntifkey="4" /label=K.\lactis\URA3 BASE COUNT 1647 a 1388 c 1253 g 1762 t ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt 1321 ctatgaggag actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga 1381 tatattcttt ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg 1441 tatattcgta tctgtatgta cacatatact atatacagga aaagataagc aagagagagg 1501 atatcaacta cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat 1561 cggcgcttag tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta 1621 actcctatag tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc 1681 tatcctcttt aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat 1741 ctatttgccg tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt 1801 cctcgggcag agaaactcgc aggcaacttg ctctcgaagt ggtcacgtat taagtcctca 1861 gcgagctcgc atggaatgcg tgcgatgagc gacctcatgc tatacctgag aaagcaacct 1921 gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact 1981 ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa tcataaatca 2041 taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacggaat gcgtgcgatc 2101 gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc 2161 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc 2221 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga 2281 aggttttggg acgctcgaag atcgcgtcag ctgaagcttc gtacgctgca ggtcgacaac 2341 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatcccaat 2401 acaacagatc acgtgatctt ttgtaagatg aagttgaagt gagtgttgca ccgtgccaat 2461 gcaggtggct attagattaa atatgtgatt tgttctatta agtttcctgt ataattaatg 2521 gggagcgctg attctctttt ggtacgcttc ccatccagca tttctgtatc tttcaccttc 2581 aaccttagga tctctaccct tggcgaaaag tcctctgcca acaatgatga tatctgatcc 2641 accacttaca acttcgtcga cggttctgta ctgctgaccc aatgcatcgc ctttgtcgtc 2701 taaacctaca cctggggtca tgattagcca atcaaaccct tcttctcttc ctcccatatc 2761 gttctgagca atgaacccaa taacgaaatc tttatcactc tttgcaatat caacggtacc 2821 cttagtatat tcaccgtgtg ctagagaacc cttggaagac aattcagcaa gcatcaataa 2881 tccccttggt tctttggtga cctcttgcgc accttgtttc aagccagcaa caataccagc 2941 accagtaacc ccgtgggcgt tggtgatatc agaccattct gcgatacggt aaacgcccga 3001 tgtatattgt aatttgactg tgttaccgat atcggcgaat tttctgtcct caaatatcaa 3061 gaacttgtat ttctctgcca atgctttcaa tggaacgaca gtaccctcat aactgaaatc 3121 atccaagata tcaacgtgtg ttttcaaaag gcaaatgtat ggacccaacg tttcaacaag 3181 tttcaatagc tcatcagtcg aacgaacgtc aagagaagca cacaaattgg tcttcttttc 3241 atccattaaa cgtaaaagtt tcgatgcaac cggacttgca tgagtctcag ctctactggt 3301 atatgatttt gtggacatgg tgcaactaat tgacgggagt gtattgacgc tggcgtactg 3361 gctttcacaa aatggcccaa tcacaaccac atcttagata gttgaaatga ctttagataa 3421 catcaattga gatgagctta atcatgtcaa agctaaaagt gtcaccatga acgacaattc 3481 ttaagcaaat cacgtgatat agatccacga ataaccacca tttgatgctc gaggcaagta 3541 atgtgtgtaa aaaaatgcgt taccaccatc caatgcagac cgatcttcta cccagaatca 3601 catatattta tgtaccgagt accttttttc tatcttccaa ttgcttctcc catatgattg 3661 tctccgtaag ctcgaaattt ctaagttgga ttttaatctt cacgcaggat gacagttcga 3721 tgagcttctg aggagtgttt agaacataat cagtttatcc atggtctatc tcttcttgtc 3781 gctttttctc ctcgatagaa cctaaataaa acgagctctc gagaaccctt aatataactt 3841 cgtataatgt atgctatacg aagttattag gtgatatcag atccactagt ggcctatgca 3901 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 3961 cgtgactggg aaaaccctgg cgttacccct gcaggactag tgctgaggca ttaatcctgc 4021 ataatcggcc tcacagaggg atcccgttac ccatctatgc tgaagattta tcatactatt 4081 cctccgctcg tttctttttt cagtgaggtg tgtcgtgaaa gaaaacccac aattaaactt 4141 tcaacaaccg ggcgactagg aagagagtag tgggcgcgga tgacgaaggc taaggtcact 4201 tcttcgtttc ctttattggg gtttccgtgt agccttcccc tgaatagtgt gggacgtttt 4261 atgagaagcc gtaagaaata ggcaaattga gttatgacaa gtagacatga tgccgcagcc 4321 ttgcctgact ttacgtctcc ttcatgaata agtttttcta tcgagttctt ttcctttttt 4381 cgccttaatt agctcaatta agcctgtcct cactactttt ctttttctta tcggctttgt 4441 gccacaccta accttcgaat gctgttttat tccgttctta catgggatgg taatgccttg 4501 gcgagattaa gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa gttcctatac 4561 tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa atttattcta 4621 aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta ttatcgttga 4681 catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag atttattttc 4741 ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga 4801 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt 4861 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacaggcgcc 4921 cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa cccgccgaag 4981 cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc ccagggggcc 5041 cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa cgcaaaaagg 5101 ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact ctcgccttcc 5161 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 5221 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 5281 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5521 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtgggctaac 5761 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5941 ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt 6001 tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctttt //