ID pCfB255 standard; circular DNA; ; 6050 BP. DE LMBP 9494. CC Accession number: LMBP 9494 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-APR-2015 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1318..1848 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\X\integration\targeting\sequence FT terminator complement(1951..2052) FT /vntifkey="43" FT /label=S.\cerevisiae\ADH1\terminator FT terminator 2110..2300 FT /vntifkey="43" FT /label=S.\cerevisiae\CYC1\terminator FT misc_feature 4016..4506 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\X\integration\targeting\sequence FT rep_origin complement(5156..5555) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(6049..859) FT /vntifkey="4" FT /label=ampicillin\resistance FT misc_feature complement(2348..2381) FT /vntifkey="21" FT /label=loxP FT misc_feature complement(3834..3867) FT /vntifkey="21" FT /label=loxP FT promoter complement(3319..3814) FT /vntifkey="29" FT /label=K.\lactis\URA3\promoter FT terminator complement(2397..2514) FT /vntifkey="43" FT /label=K.\lactis\URA3\terminator FT promoter complement(3913..3929) FT /vntifkey="30" FT /label=T7\promoter FT terminator complement(1163..1200) FT /vntifkey="43" FT /label=T1\terminator FT misc_feature 1244..1277 FT /vntifkey="21" FT /label=FRT FT misc_feature complement(4548..4581) FT /vntifkey="21" FT /label=FRT FT promoter complement(4582..4596) FT /vntifkey="30" FT /label=T7\promoter FT misc_feature 2082..2110 FT /vntifkey="21" FT /label=USER\cloning\cassette FT CDS complement(2515..3318) FT /vntifkey="4" FT /label=K.\lactis\URA3 SQ Sequence 6050 BP; 1647 A; 1388 C; 1253 G; 1762 t; accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 60 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 120 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 180 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 240 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 300 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 360 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 420 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 480 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 540 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 600 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 660 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 720 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 780 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 840 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 900 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 960 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1020 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1080 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1140 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1200 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1260 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt 1320 ctatgaggag actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga 1380 tatattcttt ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg 1440 tatattcgta tctgtatgta cacatatact atatacagga aaagataagc aagagagagg 1500 atatcaacta cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat 1560 cggcgcttag tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta 1620 actcctatag tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc 1680 tatcctcttt aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat 1740 ctatttgccg tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt 1800 cctcgggcag agaaactcgc aggcaacttg ctctcgaagt ggtcacgtat taagtcctca 1860 gcgagctcgc atggaatgcg tgcgatgagc gacctcatgc tatacctgag aaagcaacct 1920 gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact 1980 ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa tcataaatca 2040 taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacggaat gcgtgcgatc 2100 gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc 2160 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc 2220 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga 2280 aggttttggg acgctcgaag atcgcgtcag ctgaagcttc gtacgctgca ggtcgacaac 2340 ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag agatcccaat 2400 acaacagatc acgtgatctt ttgtaagatg aagttgaagt gagtgttgca ccgtgccaat 2460 gcaggtggct attagattaa atatgtgatt tgttctatta agtttcctgt ataattaatg 2520 gggagcgctg attctctttt ggtacgcttc ccatccagca tttctgtatc tttcaccttc 2580 aaccttagga tctctaccct tggcgaaaag tcctctgcca acaatgatga tatctgatcc 2640 accacttaca acttcgtcga cggttctgta ctgctgaccc aatgcatcgc ctttgtcgtc 2700 taaacctaca cctggggtca tgattagcca atcaaaccct tcttctcttc ctcccatatc 2760 gttctgagca atgaacccaa taacgaaatc tttatcactc tttgcaatat caacggtacc 2820 cttagtatat tcaccgtgtg ctagagaacc cttggaagac aattcagcaa gcatcaataa 2880 tccccttggt tctttggtga cctcttgcgc accttgtttc aagccagcaa caataccagc 2940 accagtaacc ccgtgggcgt tggtgatatc agaccattct gcgatacggt aaacgcccga 3000 tgtatattgt aatttgactg tgttaccgat atcggcgaat tttctgtcct caaatatcaa 3060 gaacttgtat ttctctgcca atgctttcaa tggaacgaca gtaccctcat aactgaaatc 3120 atccaagata tcaacgtgtg ttttcaaaag gcaaatgtat ggacccaacg tttcaacaag 3180 tttcaatagc tcatcagtcg aacgaacgtc aagagaagca cacaaattgg tcttcttttc 3240 atccattaaa cgtaaaagtt tcgatgcaac cggacttgca tgagtctcag ctctactggt 3300 atatgatttt gtggacatgg tgcaactaat tgacgggagt gtattgacgc tggcgtactg 3360 gctttcacaa aatggcccaa tcacaaccac atcttagata gttgaaatga ctttagataa 3420 catcaattga gatgagctta atcatgtcaa agctaaaagt gtcaccatga acgacaattc 3480 ttaagcaaat cacgtgatat agatccacga ataaccacca tttgatgctc gaggcaagta 3540 atgtgtgtaa aaaaatgcgt taccaccatc caatgcagac cgatcttcta cccagaatca 3600 catatattta tgtaccgagt accttttttc tatcttccaa ttgcttctcc catatgattg 3660 tctccgtaag ctcgaaattt ctaagttgga ttttaatctt cacgcaggat gacagttcga 3720 tgagcttctg aggagtgttt agaacataat cagtttatcc atggtctatc tcttcttgtc 3780 gctttttctc ctcgatagaa cctaaataaa acgagctctc gagaaccctt aatataactt 3840 cgtataatgt atgctatacg aagttattag gtgatatcag atccactagt ggcctatgca 3900 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 3960 cgtgactggg aaaaccctgg cgttacccct gcaggactag tgctgaggca ttaatcctgc 4020 ataatcggcc tcacagaggg atcccgttac ccatctatgc tgaagattta tcatactatt 4080 cctccgctcg tttctttttt cagtgaggtg tgtcgtgaaa gaaaacccac aattaaactt 4140 tcaacaaccg ggcgactagg aagagagtag tgggcgcgga tgacgaaggc taaggtcact 4200 tcttcgtttc ctttattggg gtttccgtgt agccttcccc tgaatagtgt gggacgtttt 4260 atgagaagcc gtaagaaata ggcaaattga gttatgacaa gtagacatga tgccgcagcc 4320 ttgcctgact ttacgtctcc ttcatgaata agtttttcta tcgagttctt ttcctttttt 4380 cgccttaatt agctcaatta agcctgtcct cactactttt ctttttctta tcggctttgt 4440 gccacaccta accttcgaat gctgttttat tccgttctta catgggatgg taatgccttg 4500 gcgagattaa gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa gttcctatac 4560 tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa atttattcta 4620 aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta ttatcgttga 4680 catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag atttattttc 4740 ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga 4800 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt 4860 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacaggcgcc 4920 cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa cccgccgaag 4980 cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc ccagggggcc 5040 cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa cgcaaaaagg 5100 ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact ctcgccttcc 5160 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 5220 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 5280 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5340 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5400 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5460 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5520 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5580 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5640 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5700 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtgggctaac 5760 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5820 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5880 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5940 ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt 6000 tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctttt 6050 //