LOCUS       pCfB257                 6993 bp    DNA     circular UNA 26-MAR-2015
DEFINITION  LMBP 9495.
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     Accession number: LMBP 9495
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 26-MAR-2015
            
            
            
COMMENT     VNTDATE|728842887|
COMMENT     VNTNAME|pCfB257|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2348 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5207 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 3 1321 0 3308 0 4500 0 0 
COMMENT            "") #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4979 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 4 125 0 1110 0 5863 0 6468 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2143 2333 
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COMMENT        #8=(CFSignal (CObList) "S. cerevisiae chrom. X fragment" 21 0 0 4889 
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COMMENT        #9=(CFSignal (CObList) "loxP" 21 0 1 2381 2414 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 6099 6498 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "T1 terminator" 43 0 1 1163 1200 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "T7 promoter" 30 0 1 5525 5539 0 (CStringList) 
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COMMENT        #21=(CFSignal (CObList) "USER" 21 0 0 2120 2143 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCfB257'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/19/15 04:21" 1) 
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COMMENT                          "Last Modified: 03/26/15 10:31" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6993 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 20-MAR-2015" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
COMMENT                          1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
COMMENT                          1) 
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COMMENT                          "Associated Genes:  LEU2" 1))) "Standard Fields") 
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FEATURES             Location/Qualifiers
     misc_feature    1318..1881
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\X\fragment
     terminator      complement(1984..2085)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2143..2333
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    4889..5448
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\X\fragment
     misc_feature    complement(2381..2414)
                     /vntifkey="21"
                     /label=loxP
     CDS             complement(2568..3656)
                     /vntifkey="4"
                     /label=K.\lactis\LEU2
     misc_feature    complement(4703..4736)
                     /vntifkey="21"
                     /label=loxP
     terminator      complement(2430..2567)
                     /vntifkey="43"
                     /label=K.\lactis\LEU2\terminator
     promoter        complement(3657..4681)
                     /vntifkey="29"
                     /label=K.\lactis\LEU2\promoter
     rep_origin      complement(6099..6498)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6992..859)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(1163..1200)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1244..1277
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(5491..5524)
                     /vntifkey="21"
                     /label=FRT
     promoter        complement(4786..4802)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(5525..5539)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    2120..2143
                     /vntifkey="21"
                     /label=USER
BASE COUNT     1973 a      1516 c      1525 g      1979 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga 
     1321 gatctttgtg ttcggttacc cggctcagat cctaacttgg tatgtttatt cgtataagtt 
     1381 actgttgtcc acaggcaata ctctgcagaa aattaaaacg gcattaatgc taggacaacc 
     1441 agaattgtta ctactgtatg tgcgatagtt gataactgca acattatgcc cggtatattc 
     1501 tcaaaaaacc ctattactgc atacgaagaa atcgcaagag aaatctttcg gtttggaaaa 
     1561 gctcactgtg aggttccttg gagccaatag taatacagca caatccaagg aaaaatctgg 
     1621 cctatatgca aggaaggaga gatagtcaaa agcattcttt cccctagaag ttggtgcata 
     1681 tatggcatcg ttaaaacata ttacccccaa aatttcttct ctaaacgatg tgcttggcct 
     1741 ttgttttggt ttttgatgtc ggtcgtttga ggccccttgc ggaaaatcga gatcgccgaa 
     1801 tggcacgcga gggaagggaa ataaggttta aaggcactga aacaataggc aagaagtagg 
     1861 cgagagccga catacgagac tattaagtcc tcagcgagct cgcatggaat gcgtgcgatg 
     1921 agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa 
     1981 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt 
     2041 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc 
     2101 aacaacgtat ctaccaacgg aatgcgtgcg atcgcgtgca ttcatccgct ctaaccgaaa 
     2161 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag 
     2221 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg 
     2281 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aagatcgcgt 
     2341 cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt ataatgtatg 
     2401 ctatacgaag ttattaggtc tagagatccg caggctaacc ggaacctgta ttatttagtt 
     2461 tatgctacgt taaataaaga cctttcgttc acataactga atgtgtaatg gccttgagat 
     2521 ttcaagcata ccaagttggt ggagacgggg tcgttacaaa agactcttta agccaagatt 
     2581 tccttgacag ccttggcgat agcatcgcca acctcagtgg tagagttaga accaccaagg 
     2641 tcaccggttc tgacacctgc atccaagaca tttctaacag cttcttcaag agccctacct 
     2701 tcttcaacca aatccaagga taacttcaac atcatagctg cagataagat ggtagcaatt 
     2761 gggttaacct tgtttgctgg taaatctggg gcagaaccat gacatggttc gtacaaaccg 
     2821 aatgccttgt tagtgtcagg tagggaagct agagatgcag aaggtaataa acccaaagaa 
     2881 cctggaataa cagaggcttc atcggagata atatcaccaa acatgttgtt ggtaataaca 
     2941 acaccgttta gcttagttgg tgatttaacc aaaatcatag cagcagagtc gatcaattgg 
     3001 tgctgaacag ttaattgtgg gaactcagtc ttgatggttt cttcaacagt ctttctccac 
     3061 aatctggaag aggcaagcac gttagccttg tcaagagacc agattggtaa tggtgggttt 
     3121 tgttgcaatg ccaagaaagc agccattctt gtaattcttt gaacttcagg aacactgtat 
     3181 ttctcagagt cccaagcaac tccgtcacct tcatcttctt ttctttcacc aaagtagata 
     3241 ccaccaacca attctctaac gacgacgaaa tcggtaccct ttgcatattc aggcttcaaa 
     3301 ggagaaagat ctagtaaaga atcagaagca aagttacatg gtctcaagtt ggcgtataga 
     3361 cccaattcct ttctgatctt caatagacct tgttctggtc taactgcgcc cgtaccccat 
     3421 tttggaccac caacagcacc tagtaagaca gcatcggctt tcttagaggc ttctagagct 
     3481 tcatctggta aaggagtgcc agtggcatcg atggcagcac ccccgatcaa gtgatgttgg 
     3541 aaattgaact taatttctgg acggacttca gcaatggcat tcaagacctt aatagcttcg 
     3601 tcagtaactt ctttaccgac gtgatcaccc ggtaggacaa cgatattctt agacatggta 
     3661 taatctgtgt agtgtgggat actttttact tcttcaaata ggtatcaaca ataaaaataa 
     3721 atcgaagcaa atgtaggaat gcgttaaagc agatgtactt acttagagta catagatata 
     3781 tttatataat tcaatatata aaagtatatg aagcatctga tgttgaacct gtcatgactc 
     3841 taaactggaa tgggtagtta tgcttctgaa ggttttccgt tagaaaatga gtgcagggat 
     3901 caggcccgga accggtttta gcctgaaagg aaaagaaaaa aatgcggtga ttactagcac 
     3961 gtgactgcgc tgaattggaa tccaccacaa gtacagcgaa tggcaagagg gaaggggaat 
     4021 atattgtgcc tggcggtagt ctgctatatc aaatgttaaa cataactaaa tgcagtagtg 
     4081 aatagagtaa tgtatttcta catatgtggg tagggccaat gggagcccga tgtctattgt 
     4141 acagatattt tccttttata gttaatcaat tcaacttgga aatcattgag tcgatatcta 
     4201 cttggataac tggagccgtg gcagcgtttg aaatttgacg agtcttcttg ttttgttcgc 
     4261 gcattttcag tttattcaaa cgggagatgg caccgaatcc actgacttct ctgttcaatt 
     4321 tgaatggtaa taggaagaaa gtacatttcg agaaattttc gcccaacgtc gaaatctcat 
     4381 ggatgatatc ctcgatacaa atgacacctt catcacctaa tttctcttca acgatattgt 
     4441 tgtcattcaa gattatctct tttggttctt tatcttctgg cctttgccac attattctag 
     4501 atctcttttg gattaaagaa cggatagatg caagtgaagg ttgtccgata acaatatatg 
     4561 gtgatacaag tttcaacaat gggtacacgt ccttggtaag tttgatgaaa acaccagtgt 
     4621 tcaactcaac aagtctcaaa agagataaca ctttgtaagc ctttgctggg atcttcacag 
     4681 cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga agttattagg 
     4741 taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag tgagtcgtat 
     4801 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     4861 cctgcaggac tagtgctgag gcattaattg tgtccgcgtt tctaaggcca cttttcaatg 
     4921 aaacggatat tgatatgcta gtaaaaggac gagctcaaga gcgaaaatat aagtaaagaa 
     4981 ttcgagtgca cttgtctcca tgcagcaaga tttcatatga gtctttttta tctttttact 
     5041 ttttacatta cacgatatgc actttatgaa aatttaacga ggttggaagc cggataatca 
     5101 accaaaatca ggcacgaagg cacactcgta tatgcatgtt gttgaaactc tgttacgctg 
     5161 aactaacaat cacacatgta gaggtcaccg ggaaaagttg cgaccccatg gaaggtcgat 
     5221 ctcttcgttt ggctttgctt ggctggcggc attgcgcttc ttcgcttata cccgtctctt 
     5281 gacgctcgag ctcgttcatt gagatacctt tattcttgca cattttctgg cttttttcgc 
     5341 tactcgggta catgtaatca tgcacacaga aggtgctgta gggtgaaagt tcctttgtgc 
     5401 tgtcgtttgt ttttaatgcc aaactttccg gtgatcaata accacctcat taagacctca 
     5461 gcgcggccgc aaatttaaat aaaatgaagt gaagttccta tactttctag agaataggaa 
     5521 cttctatagt gagtcgaata agggcgacac aaaatttatt ctaaatgcat aataaatact 
     5581 gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata 
     5641 tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa 
     5701 actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt 
     5761 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat 
     5821 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat attctgacaa 
     5881 atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt 
     5941 tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt aagactggcc 
     6001 gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg tcaggggcct 
     6061 tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct cgctcactga 
     6121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     6181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     6241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     6301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     6361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     6421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     6481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     6541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     6601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     6661 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag 
     6721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     6781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     6841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     6901 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagcttgcgc 
     6961 cgtcccgtca agtcagcgta atgctctgct ttt 
//
