LOCUS       pCfB258                 5864 bp    DNA     circular UNA 20-MAR-2015
DEFINITION  LMBP 9496.
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     Accession number: LMBP 9496
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-MAR-2015
            
            
COMMENT     VNTDATE|728845260|
COMMENT     VNTNAME|pCfB258|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCfB258" 0 5864 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        (CWordArray) (CStringList "BglII" "BstXI" "HaeII" "NsiI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2383 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 2 2430 0 2834 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 125 0 1110 0 3340 0 4734 
COMMENT            0 5339 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NsiI" "atgcat" 6 0 1 4441 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 672 0 0 "")) 
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COMMENT            1839 0 (CStringList) (CStringList) 1 1 1 1 "1318..1839") 
COMMENT        #6=(CFSignal (CObList) "S. cerevisiae chrom. X fragment" 21 0 0 3869 
COMMENT            4319 0 (CStringList) (CStringList) 1 1 1 1 "3869..4319") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 5863 859 0 
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COMMENT        #8=(CFSignal (CObList) "E. gossypii TEF1 promoter" 29 0 0 2392 2770 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2373..2770") 
COMMENT        #9=(CFSignal (CObList) "S. pombe HIS5" 4 0 0 2771 3424 0 (CStringList) 
COMMENT            (CStringList "/gene=\"HIS5\"" "/codon_start=1" "/transl_table=11" 
COMMENT             "/product=\"imidazoleglycerol-phosphate dehydratase\"" 
COMMENT             "/protein_id=\"AAG34536.1\"" "/db_xref=\"GI:11344900\"") 1 1 1 1 
COMMENT            "2771..3424") 
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COMMENT        #11=(CFSignal (CObList) "T1 terminator" 43 0 1 1163 1200 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "FRT" 21 0 0 1244 1277 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "FRT" 21 0 1 4362 4395 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1942 2043 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2101 2291 
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COMMENT        #16=(CFSignal (CObList) "loxP" 21 0 1 2339 2372 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "loxP" 21 0 1 3687 3720 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "T7 promoter" 30 0 1 3766 3782 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "T7 promoter" 30 0 1 4396 4410 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 4970 5369 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "USER" 21 0 0 2078 2101 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT        #22=(CFSignal (CObList) "LMBP 9496" 2 0 0 0 0 0 (CStringList) 
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COMMENT        #23=(CFSignal (CObList) "Last modified: 20-MAR-2015" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCfB258'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/19/15 05:01" 1) 
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COMMENT                          "Last Modified: 03/20/15 09:39" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5864 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-2015" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
COMMENT                          1) 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
COMMENT                          1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
COMMENT                          1) 
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COMMENT                          "Associated Genes:  HIS5" 1))) "Standard Fields") 
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COMMENT                          "Replication Origin (1 total)") 
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COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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FEATURES             Location/Qualifiers
     gene            complement(1..859)
                     /vntifkey="60"
     misc_feature    1318..1839
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\X\fragment
     misc_feature    3869..4319
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\X\fragment
     CDS             complement(5863..859)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        2392..2770
                     /vntifkey="29"
                     /label=E.\gossypii\TEF1\promoter
     CDS             2771..3424
                     /gene="HIS5"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="AAG34536.1"
                     /db_xref="GI:11344900"
                     /vntifkey="4"
                     /label=S.\pombe\HIS5
     terminator      3425..3659
                     /vntifkey="43"
                     /label=E.\gossypii\TEF1\terminator
     terminator      complement(1163..1200)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1244..1277
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4362..4395)
                     /vntifkey="21"
                     /label=FRT
     terminator      complement(1942..2043)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2101..2291
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    complement(2339..2372)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(3687..3720)
                     /vntifkey="21"
                     /label=loxP
     promoter        complement(3766..3782)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4396..4410)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(4970..5369)
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_feature    2078..2101
                     /vntifkey="21"
                     /label=USER
BASE COUNT     1620 a      1323 c      1297 g      1624 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatccc 
     1321 aaagctaaga gtcccatttt attcttctat atgtatattt tcgatactct aaaccaccct 
     1381 acaatgtagc cctatactaa atctgctcaa ttttcagctt ctacaagtga ctcgagacca 
     1441 cgtggaaaga tccaactact ccagcacaac gattcaatat aatcgattgc tccactcata 
     1501 agaggcaaga acaagcttca acttttggta agccgccgtt tataaacagg gaagatgtcc 
     1561 tttgtcaagg gaggcacaga gcatggccaa tttggcaaat tgcaggtttt tctgagtgaa 
     1621 aaatgaaaaa gcattgtagt agagtcggct cactgaaaaa ccggggagga cgaaaaggtt 
     1681 tccagccaca gttgtagtca cgtgcgcgcc atgctgacta atggcagccg tcgttgggca 
     1741 gaagagaatt agtatggtac aggatacgct aattgcgctc caactaccaa ggttgttgag 
     1801 ggaacactgg ggcaataggc tgtcgccatt caagagcaga ttaagtcctc agcgagctcg 
     1861 catggaatgc gtgcgatgag cgacctcatg ctatacctga gaaagcaacc tgacctacag 
     1921 gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt 
     1981 gtatacactt atttttttta taacttattt aataataaaa atcataaatc ataagaaatt 
     2041 cgcttattta gaagtgtcaa caacgtatct accaacggaa tgcgtgcgat cgcgtgcatt 
     2101 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt 
     2161 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc 
     2221 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg 
     2281 gacgctcgaa gatcgcgtca gctgaagctt cgtacgctgc aggtcgacaa cccttaatat 
     2341 aacttcgtat aatgtatgct atacgaagtt attaggtcta gagatctgtt tagcttgcct 
     2401 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc 
     2461 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc 
     2521 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt 
     2581 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt 
     2641 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct 
     2701 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac 
     2761 ataaacaacc atgggtagga gggcttttgt agaaagaaat acgaacgaaa cgaaaatcag 
     2821 cgttgccatc gctttggaca aagctccctt acctgaagag tcgaatttta ttgatgaact 
     2881 tataacttcc aagcatgcaa accaaaaggg agaacaagta atccaagtag acacgggaat 
     2941 tggattcttg gatcacatgt atcatgcact ggctaaacat gcaggctgga gcttacgact 
     3001 ttactcaaga ggtgatttaa tcatcgatga tcatcacact gcagaagata ctgctattgc 
     3061 acttggtatt gcattcaagc aggctatggg taactttgcc ggcgttaaaa gatttggaca 
     3121 tgcttattgt ccacttgacg aagctctttc tagaagcgta gttgacttgt cgggacggcc 
     3181 ctatgctgtt atcgatttgg gattaaagcg tgaaaaggtt ggggaattgt cctgtgaaat 
     3241 gatccctcac ttactatatt ccttttcggt agcagctgga attactttgc atgttacctg 
     3301 cttatatggt agtaatgacc atcatcgtgc tgaaagcgct tttaaatctc tggctgttgc 
     3361 catgcgcgcg gctactagtc ttactggaag ttctgaagtc ccaagcacga agggagtgtt 
     3421 gtaaagagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt 
     3481 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc 
     3541 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat 
     3601 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt 
     3661 taaacgagct ctcgagaacc cttaatataa cttcgtataa tgtatgctat acgaagttat 
     3721 taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag tgagtcgtat 
     3781 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     3841 cctgcaggac tagtgctgag gcattaataa caggcatggg aagattcgct tttttttttt 
     3901 gaattacaat agtatgtctg atgtctgcaa gaagtaacag gcgtgtgcac aagaatacgt 
     3961 gtgtgtgcgt aagcgtatgc actggtggca taacttatct aagaagtata tatcactgac 
     4021 atagaaatgt agatatacag gtatttttct cgataatcga taaaaatctc gtcgcgctga 
     4081 accaaacttg gtggttacgg agagtttttc tctcatcatt actgtctttc gcattgattt 
     4141 cccctttgac cgataaaatc ccttggattc ataagattaa acaaagaggt gatcaaagag 
     4201 aaccctgtga aagtttatgt ttataaccgg gcataaagtg aactagacac tttcaagaag 
     4261 ccaaccaaag catgagtaac gaagcttacc agcatgatca taccgtaaat cctcaccaga 
     4321 ttaagacctc agcgcggccg caaatttaaa taaaatgaag tgaagttcct atactttcta 
     4381 gagaatagga acttctatag tgagtcgaat aagggcgaca caaaatttat tctaaatgca 
     4441 taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg ttgacatgta 
     4501 taattttgat atcaaaaact gattttccct ttattatttt cgagatttat tttcttaatt 
     4561 ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag atgaatagtt 
     4621 taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt 
     4681 ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg cgcccttaaa 
     4741 tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc gaagcgggtt 
     4801 tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccaggg ggcccgagct 
     4861 taagactggc cgtcgtttta caacacagaa agagtttgta gaaacgcaaa aaggccatcc 
     4921 gtcaggggcc ttctgcttag tttgatgcct ggcagttccc tactctcgcc ttccgcttcc 
     4981 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     5041 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     5101 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     5161 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     5221 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     5281 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     5341 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     5401 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     5461 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     5521 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtgggc taactacggc 
     5581 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     5641 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     5701 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     5761 acggggtctg acgctcagtg gaacgacgcg cgcgtaactc acgttaaggg attttggtca 
     5821 tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc tttt 
//
