LOCUS       pCfB259                 6972 bp    DNA     circular UNA  1-APR-2015
DEFINITION  LMBP 9497.
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     Accession number: LMBP 9497
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 1-APR-2015
            
            
            
COMMENT     VNTDATE|728908285|
COMMENT     VNTNAME|pCfB259|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        #1=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 125 0 1110 0 5073 0 5842 
COMMENT            0 6447 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 443 0 2083 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 553 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 4638 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NsiI" "atgcat" 6 0 1 5549 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2093 2283 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6971 859 0 
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COMMENT        #9=(CFSignal (CObList) "K. lactis LEU2 terminator" 43 0 1 2380 2517 0 
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COMMENT        #10=(CFSignal (CObList) "T1 terminator" 43 0 1 1163 1200 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "loxP" 21 0 1 4653 4686 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "T7 promoter" 30 0 1 4736 4752 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "T7 promoter" 30 0 1 5504 5518 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 6078 6477 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "USER cloning cassette" 21 0 0 2066 2093 0 
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COMMENT             "S. cerevisiae chrom. XII integration targeting sequence" 21 0 0 
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COMMENT        #22=(CFSignal (CObList) "K. lactis LEU2" 4 0 1 2518 3606 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCfB259'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/20/15 10:31" 1) 
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COMMENT                          "Last Modified: 04/01/15 12:29" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6972 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 26-MAR-2015" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
COMMENT                          1) 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
COMMENT                          1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
COMMENT                          1) 
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COMMENT                                 "" 1))) "LMBP 9497") 
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COMMENT                                        "   complement(3607..4631)" 1))) 
COMMENT                                 "K. lactis LEU2 promoter"))) 
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FEATURES             Location/Qualifiers
     misc_feature    1317..1832
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence
     terminator      complement(1934..2035)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2093..2283
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    4838..5429
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence
     CDS             complement(6971..859)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(2380..2517)
                     /vntifkey="43"
                     /label=K.\lactis\LEU2\terminator
     promoter        complement(3607..4631)
                     /vntifkey="29"
                     /label=K.\lactis\LEU2\promoter
     terminator      complement(1163..1200)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1244..1277
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(5470..5503)
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2066..2093
                     /vntifkey="21"
                     /label=USER\cloning\cassette
     misc_feature    complement(2331..2364)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(4653..4686)
                     /vntifkey="21"
                     /label=loxP
     promoter        complement(4736..4752)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(5504..5518)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(6078..6477)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2518..3606)
                     /vntifkey="4"
                     /label=K.\lactis\LEU2
BASE COUNT     1989 a      1485 c      1489 g      2009 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgtt 
     1321 gagctctgtc cttcatggac tttttggacc acttcttctt ggacttctta ccaccagcaa 
     1381 gagcggcagc ggctttggca gctttagaca attgttgctt tggaggcatg ttatattatg 
     1441 ttctgagacg taagaaaggg tgaaaattga tgttagtgtc aaaaattata ttacaaaata 
     1501 cgcagagata ttctagttcc tttgatgaat gaatctttca gaaaaaaaag tcaaagcaaa 
     1561 agcaaaatgg cctgcagact aaactgtatg gtggtcttgg aatgataaag atctgtttaa 
     1621 tagatttagt agatacaata gcacatctca ttacccagtt atgattgacg tcattctgag 
     1681 ttacaatgat cttagcgacc tgtgctcatt atttgctcca ctaattctaa ttttcctcgc 
     1741 ctttcatatt tcgtatcttt attctatatc ctaaaatttt tttggcaaat cccagatttg 
     1801 gctttgattt tggcatcggt tcggttcttt cattaagtcc tcagcgagct cgcatggaat 
     1861 gcgtgcgatg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt 
     1921 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac 
     1981 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt 
     2041 tagaagtgtc aacaacgtat ctaccaacgg aatgcgtgcg atcgcgtgca ttcatccgct 
     2101 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 
     2161 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 
     2221 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 
     2281 aagatcgcgt cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt 
     2341 ataatgtatg ctatacgaag ttattaggtc tagagatccg caggctaacc ggaacctgta 
     2401 ttatttagtt tatgctacgt taaataaaga cctttcgttc acataactga atgtgtaatg 
     2461 gccttgagat ttcaagcata ccaagttggt ggagacgggg tcgttacaaa agactcttta 
     2521 agccaagatt tccttgacag ccttggcgat agcatcgcca acctcagtgg tagagttaga 
     2581 accaccaagg tcaccggttc tgacacctgc atccaagaca tttctaacag cttcttcaag 
     2641 agccctacct tcttcaacca aatccaagga taacttcaac atcatagctg cagataagat 
     2701 ggtagcaatt gggttaacct tgtttgctgg taaatctggg gcagaaccat gacatggttc 
     2761 gtacaaaccg aatgccttgt tagtgtcagg tagggaagct agagatgcag aaggtaataa 
     2821 acccaaagaa cctggaataa cagaggcttc atcggagata atatcaccaa acatgttgtt 
     2881 ggtaataaca acaccgttta gcttagttgg tgatttaacc aaaatcatag cagcagagtc 
     2941 gatcaattgg tgctgaacag ttaattgtgg gaactcagtc ttgatggttt cttcaacagt 
     3001 ctttctccac aatctggaag aggcaagcac gttagccttg tcaagagacc agattggtaa 
     3061 tggtgggttt tgttgcaatg ccaagaaagc agccattctt gtaattcttt gaacttcagg 
     3121 aacactgtat ttctcagagt cccaagcaac tccgtcacct tcatcttctt ttctttcacc 
     3181 aaagtagata ccaccaacca attctctaac gacgacgaaa tcggtaccct ttgcatattc 
     3241 aggcttcaaa ggagaaagat ctagtaaaga atcagaagca aagttacatg gtctcaagtt 
     3301 ggcgtataga cccaattcct ttctgatctt caatagacct tgttctggtc taactgcgcc 
     3361 cgtaccccat tttggaccac caacagcacc tagtaagaca gcatcggctt tcttagaggc 
     3421 ttctagagct tcatctggta aaggagtgcc agtggcatcg atggcagcac ccccgatcaa 
     3481 gtgatgttgg aaattgaact taatttctgg acggacttca gcaatggcat tcaagacctt 
     3541 aatagcttcg tcagtaactt ctttaccgac gtgatcaccc ggtaggacaa cgatattctt 
     3601 agacatggta taatctgtgt agtgtgggat actttttact tcttcaaata ggtatcaaca 
     3661 ataaaaataa atcgaagcaa atgtaggaat gcgttaaagc agatgtactt acttagagta 
     3721 catagatata tttatataat tcaatatata aaagtatatg aagcatctga tgttgaacct 
     3781 gtcatgactc taaactggaa tgggtagtta tgcttctgaa ggttttccgt tagaaaatga 
     3841 gtgcagggat caggcccgga accggtttta gcctgaaagg aaaagaaaaa aatgcggtga 
     3901 ttactagcac gtgactgcgc tgaattggaa tccaccacaa gtacagcgaa tggcaagagg 
     3961 gaaggggaat atattgtgcc tggcggtagt ctgctatatc aaatgttaaa cataactaaa 
     4021 tgcagtagtg aatagagtaa tgtatttcta catatgtggg tagggccaat gggagcccga 
     4081 tgtctattgt acagatattt tccttttata gttaatcaat tcaacttgga aatcattgag 
     4141 tcgatatcta cttggataac tggagccgtg gcagcgtttg aaatttgacg agtcttcttg 
     4201 ttttgttcgc gcattttcag tttattcaaa cgggagatgg caccgaatcc actgacttct 
     4261 ctgttcaatt tgaatggtaa taggaagaaa gtacatttcg agaaattttc gcccaacgtc 
     4321 gaaatctcat ggatgatatc ctcgatacaa atgacacctt catcacctaa tttctcttca 
     4381 acgatattgt tgtcattcaa gattatctct tttggttctt tatcttctgg cctttgccac 
     4441 attattctag atctcttttg gattaaagaa cggatagatg caagtgaagg ttgtccgata 
     4501 acaatatatg gtgatacaag tttcaacaat gggtacacgt ccttggtaag tttgatgaaa 
     4561 acaccagtgt tcaactcaac aagtctcaaa agagataaca ctttgtaagc ctttgctggg 
     4621 atcttcacag cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga 
     4681 agttattagg taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag 
     4741 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     4801 tggcgttacc cctgcaggac tagtgctgag gcattaatct tcccgtgaat caactgcaca 
     4861 taccttaata actagttcag tttagtgctc tgtctgagtg actgtaataa taaattttac 
     4921 aagccacttc tcatgacata tattggtaag taacttcatc aatactaatt agtctttgcc 
     4981 ggttacccat ctggcccctg acttgcgatg cttaggaagt tccatactcg cggctcttcc 
     5041 caacagtagc acatccgtga aacttctggc gctattcatt atgcagtacc aggacaagaa 
     5101 gttaaaaaaa aagctctgtt acaagttcaa tggtggtgca aggattgaag ttattatcca 
     5161 ggaggcacgg atgcaaaaga tagaagacaa attaatttcc ttaaaattca aaatgctcat 
     5221 tattgtcacg ctgtatgagc atttggtgaa gatttcactg ggaaatgttg caataatttg 
     5281 ataatcgttc gtattggatg aaactgtaac atcatctgtt tattaagtat ccgtgttatt 
     5341 agtatatcat cacatacggt gtaagaagat aacataaaga ttgagaaaca gtcatcaaat 
     5401 ataatggaag ctgaaatgcg aggattgatt aagacctcag cgcggccgca aatttaaata 
     5461 aaatgaagtg aagttcctat actttctaga gaataggaac ttctatagtg agtcgaataa 
     5521 gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt atagtttgta 
     5581 ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt 
     5641 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca 
     5701 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa 
     5761 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat 
     5821 atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc ctaaactccc 
     5881 cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac gattactcgt 
     5941 tatcagaacc gcccaggggg cccgagctta agactggccg tcgttttaca acacagaaag 
     6001 agtttgtaga aacgcaaaaa ggccatccgt caggggcctt ctgcttagtt tgatgcctgg 
     6061 cagttcccta ctctcgcctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     6121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     6181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     6241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     6301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     6361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     6421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     6481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     6541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     6601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     6661 gttcttgaag tggtgggcta actacggcta cactagaaga acagtatttg gtatctgcgc 
     6721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     6781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     6841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgcg 
     6901 cgtaactcac gttaagggat tttggtcatg agcttgcgcc gtcccgtcaa gtcagcgtaa 
     6961 tgctctgctt tt 
//
