ID pCfB259 standard; circular DNA; UNA; 6972 BP. DE LMBP 9497. CC Accession number: LMBP 9497 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-APR-2015 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1317..1832 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence FT terminator complement(1934..2035) FT /vntifkey="43" FT /label=S.\cerevisiae\ADH1\terminator FT terminator 2093..2283 FT /vntifkey="43" FT /label=S.\cerevisiae\CYC1\terminator FT misc_feature 4838..5429 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence FT CDS complement(6971..859) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(2380..2517) FT /vntifkey="43" FT /label=K.\lactis\LEU2\terminator FT promoter complement(3607..4631) FT /vntifkey="29" FT /label=K.\lactis\LEU2\promoter FT terminator complement(1163..1200) FT /vntifkey="43" FT /label=T1\terminator FT misc_feature 1244..1277 FT /vntifkey="21" FT /label=FRT FT misc_feature complement(5470..5503) FT /vntifkey="21" FT /label=FRT FT misc_feature 2066..2093 FT /vntifkey="21" FT /label=USER\cloning\cassette FT misc_feature complement(2331..2364) FT /vntifkey="21" FT /label=loxP FT misc_feature complement(4653..4686) FT /vntifkey="21" FT /label=loxP FT promoter complement(4736..4752) FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(5504..5518) FT /vntifkey="30" FT /label=T7\promoter FT rep_origin complement(6078..6477) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(2518..3606) FT /vntifkey="4" FT /label=K.\lactis\LEU2 SQ Sequence 6972 BP; 1989 A; 1485 C; 1489 G; 2009 t; accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 60 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 120 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 180 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 240 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 300 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 360 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 420 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 480 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 540 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 600 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 660 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 720 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 780 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 840 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 900 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 960 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1020 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1080 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1140 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1200 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1260 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgtt 1320 gagctctgtc cttcatggac tttttggacc acttcttctt ggacttctta ccaccagcaa 1380 gagcggcagc ggctttggca gctttagaca attgttgctt tggaggcatg ttatattatg 1440 ttctgagacg taagaaaggg tgaaaattga tgttagtgtc aaaaattata ttacaaaata 1500 cgcagagata ttctagttcc tttgatgaat gaatctttca gaaaaaaaag tcaaagcaaa 1560 agcaaaatgg cctgcagact aaactgtatg gtggtcttgg aatgataaag atctgtttaa 1620 tagatttagt agatacaata gcacatctca ttacccagtt atgattgacg tcattctgag 1680 ttacaatgat cttagcgacc tgtgctcatt atttgctcca ctaattctaa ttttcctcgc 1740 ctttcatatt tcgtatcttt attctatatc ctaaaatttt tttggcaaat cccagatttg 1800 gctttgattt tggcatcggt tcggttcttt cattaagtcc tcagcgagct cgcatggaat 1860 gcgtgcgatg agcgacctca tgctatacct gagaaagcaa cctgacctac aggaaagagt 1920 tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac 1980 ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt 2040 tagaagtgtc aacaacgtat ctaccaacgg aatgcgtgcg atcgcgtgca ttcatccgct 2100 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 2160 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 2220 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 2280 aagatcgcgt cagctgaagc ttcgtacgct gcaggtcgac aacccttaat ataacttcgt 2340 ataatgtatg ctatacgaag ttattaggtc tagagatccg caggctaacc ggaacctgta 2400 ttatttagtt tatgctacgt taaataaaga cctttcgttc acataactga atgtgtaatg 2460 gccttgagat ttcaagcata ccaagttggt ggagacgggg tcgttacaaa agactcttta 2520 agccaagatt tccttgacag ccttggcgat agcatcgcca acctcagtgg tagagttaga 2580 accaccaagg tcaccggttc tgacacctgc atccaagaca tttctaacag cttcttcaag 2640 agccctacct tcttcaacca aatccaagga taacttcaac atcatagctg cagataagat 2700 ggtagcaatt gggttaacct tgtttgctgg taaatctggg gcagaaccat gacatggttc 2760 gtacaaaccg aatgccttgt tagtgtcagg tagggaagct agagatgcag aaggtaataa 2820 acccaaagaa cctggaataa cagaggcttc atcggagata atatcaccaa acatgttgtt 2880 ggtaataaca acaccgttta gcttagttgg tgatttaacc aaaatcatag cagcagagtc 2940 gatcaattgg tgctgaacag ttaattgtgg gaactcagtc ttgatggttt cttcaacagt 3000 ctttctccac aatctggaag aggcaagcac gttagccttg tcaagagacc agattggtaa 3060 tggtgggttt tgttgcaatg ccaagaaagc agccattctt gtaattcttt gaacttcagg 3120 aacactgtat ttctcagagt cccaagcaac tccgtcacct tcatcttctt ttctttcacc 3180 aaagtagata ccaccaacca attctctaac gacgacgaaa tcggtaccct ttgcatattc 3240 aggcttcaaa ggagaaagat ctagtaaaga atcagaagca aagttacatg gtctcaagtt 3300 ggcgtataga cccaattcct ttctgatctt caatagacct tgttctggtc taactgcgcc 3360 cgtaccccat tttggaccac caacagcacc tagtaagaca gcatcggctt tcttagaggc 3420 ttctagagct tcatctggta aaggagtgcc agtggcatcg atggcagcac ccccgatcaa 3480 gtgatgttgg aaattgaact taatttctgg acggacttca gcaatggcat tcaagacctt 3540 aatagcttcg tcagtaactt ctttaccgac gtgatcaccc ggtaggacaa cgatattctt 3600 agacatggta taatctgtgt agtgtgggat actttttact tcttcaaata ggtatcaaca 3660 ataaaaataa atcgaagcaa atgtaggaat gcgttaaagc agatgtactt acttagagta 3720 catagatata tttatataat tcaatatata aaagtatatg aagcatctga tgttgaacct 3780 gtcatgactc taaactggaa tgggtagtta tgcttctgaa ggttttccgt tagaaaatga 3840 gtgcagggat caggcccgga accggtttta gcctgaaagg aaaagaaaaa aatgcggtga 3900 ttactagcac gtgactgcgc tgaattggaa tccaccacaa gtacagcgaa tggcaagagg 3960 gaaggggaat atattgtgcc tggcggtagt ctgctatatc aaatgttaaa cataactaaa 4020 tgcagtagtg aatagagtaa tgtatttcta catatgtggg tagggccaat gggagcccga 4080 tgtctattgt acagatattt tccttttata gttaatcaat tcaacttgga aatcattgag 4140 tcgatatcta cttggataac tggagccgtg gcagcgtttg aaatttgacg agtcttcttg 4200 ttttgttcgc gcattttcag tttattcaaa cgggagatgg caccgaatcc actgacttct 4260 ctgttcaatt tgaatggtaa taggaagaaa gtacatttcg agaaattttc gcccaacgtc 4320 gaaatctcat ggatgatatc ctcgatacaa atgacacctt catcacctaa tttctcttca 4380 acgatattgt tgtcattcaa gattatctct tttggttctt tatcttctgg cctttgccac 4440 attattctag atctcttttg gattaaagaa cggatagatg caagtgaagg ttgtccgata 4500 acaatatatg gtgatacaag tttcaacaat gggtacacgt ccttggtaag tttgatgaaa 4560 acaccagtgt tcaactcaac aagtctcaaa agagataaca ctttgtaagc ctttgctggg 4620 atcttcacag cgagctctcg agaaccctta atataacttc gtataatgta tgctatacga 4680 agttattagg taggtgatat cagatccact agtggcctat gcacccaatt cgccctatag 4740 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 4800 tggcgttacc cctgcaggac tagtgctgag gcattaatct tcccgtgaat caactgcaca 4860 taccttaata actagttcag tttagtgctc tgtctgagtg actgtaataa taaattttac 4920 aagccacttc tcatgacata tattggtaag taacttcatc aatactaatt agtctttgcc 4980 ggttacccat ctggcccctg acttgcgatg cttaggaagt tccatactcg cggctcttcc 5040 caacagtagc acatccgtga aacttctggc gctattcatt atgcagtacc aggacaagaa 5100 gttaaaaaaa aagctctgtt acaagttcaa tggtggtgca aggattgaag ttattatcca 5160 ggaggcacgg atgcaaaaga tagaagacaa attaatttcc ttaaaattca aaatgctcat 5220 tattgtcacg ctgtatgagc atttggtgaa gatttcactg ggaaatgttg caataatttg 5280 ataatcgttc gtattggatg aaactgtaac atcatctgtt tattaagtat ccgtgttatt 5340 agtatatcat cacatacggt gtaagaagat aacataaaga ttgagaaaca gtcatcaaat 5400 ataatggaag ctgaaatgcg aggattgatt aagacctcag cgcggccgca aatttaaata 5460 aaatgaagtg aagttcctat actttctaga gaataggaac ttctatagtg agtcgaataa 5520 gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt atagtttgta 5580 ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga ttttcccttt 5640 attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat tgtatataca 5700 aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc gaaaaagcaa 5760 cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata attctcatat 5820 atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc ctaaactccc 5880 cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac gattactcgt 5940 tatcagaacc gcccaggggg cccgagctta agactggccg tcgttttaca acacagaaag 6000 agtttgtaga aacgcaaaaa ggccatccgt caggggcctt ctgcttagtt tgatgcctgg 6060 cagttcccta ctctcgcctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6120 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6180 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6240 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6300 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6360 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6420 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6480 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 6540 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 6600 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6660 gttcttgaag tggtgggcta actacggcta cactagaaga acagtatttg gtatctgcgc 6720 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 6780 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 6840 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgcg 6900 cgtaactcac gttaagggat tttggtcatg agcttgcgcc gtcccgtcaa gtcagcgtaa 6960 tgctctgctt tt 6972 //