LOCUS       pCfB2225                6144 bp    DNA     circular SYN 28-MAY-2019
DEFINITION  LMBP 9501.
ACCESSION   .
VERSION     .
KEYWORDS    p0120 pXII-2-kanMXsyn.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 7. feb 2014 from SnapGene 2.2.1
            http://www.snapgene.com
COMMENT     Accession number: LMBP 9501
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-MAY-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728913804|
COMMENT     VNTDBDATE|-12834228|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB2225|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCfB2225" 0 6144 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "HindIII" "NcoI" "SspI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6144 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2398 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 2321 0 3330 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 2785 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 5 879 0 1735 0 3137 0 4863 
COMMENT            0 5024 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3846 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1 861 0 
COMMENT            (CStringList) (CStringList "/codon_start=1") 1 1 1 1 
COMMENT            "complement(6143..859)") 
COMMENT        #6=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1957 2058 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "complement(1955..2056)") 
COMMENT        #7=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2116 2306 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "2114..2304") 
COMMENT        #8=(CFSignal (CObList) "loxP" 21 0 1 2354 2387 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2352..2385)") 
COMMENT        #9=(CFSignal (CObList) "E. gossypii TEF1 promoter" 29 0 0 2442 2785 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2405..2783") 
COMMENT        #10=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 2786 3595 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/transl_table=11" "/gene=\"kanMX\"" 
COMMENT              "/product=\"geneticin resistance protein\"" 
COMMENT              "/db_xref=\"GI:11344910\"" "/protein_id=\"AAG34543.1\"") 1 1 1 1 
COMMENT             "2784..3593") 
COMMENT        #11=(CFSignal (CObList) "E. gossypii TEF1 terminator" 43 0 0 3598 3798 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3596..3828") 
COMMENT        #12=(CFSignal (CObList) "loxP" 21 0 1 3861 3894 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3859..3892)") 
COMMENT        #13=(CFSignal (CObList) "T1 terminator" 43 0 1 1165 1202 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1163..1200)") 
COMMENT        #14=(CFSignal (CObList) "FRT" 21 0 0 1246 1279 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1244..1277") 
COMMENT        #15=(CFSignal (CObList) "FRT" 21 0 1 4644 4677 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4642..4675)") 
COMMENT        #16=(CFSignal (CObList) "USER cloning cassette" 21 0 0 2093 2116 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2091..2114") 
COMMENT        #17=(CFSignal (CObList) "T7 promoter" 30 0 1 3940 3956 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3938..3954)") 
COMMENT        #18=(CFSignal (CObList) "T7 promoter" 30 0 1 4678 4694 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4676..4690)") 
COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 5250 5651 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5250..5649)") 
COMMENT        #20=(CFSignal (CObList) 
COMMENT             "S. cerevisiae chrom. XII integration targeting sequence" 21 0 0 
COMMENT             1320 1855 0 (CStringList) (CStringList) 1 1 1 1 "1318..1852") 
COMMENT        #21=(CFSignal (CObList) 
COMMENT             "S. cerevisiae chrom. XII integration targeting sequence" 21 0 0 
COMMENT             4039 4602 0 (CStringList) (CStringList) 1 1 1 1 "4041..4599")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "LMBP 9501" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 28-MAY-2019" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #25=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pCfB2225" 2) 5 "" 
COMMENT                   0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pCfB2225'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9501." 
COMMENT                          1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/20/15 12:03" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/28/19 03:03" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6144 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Keywords: p0120 pXII-2-kanMXsyn" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-MAR-2015" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
COMMENT                          1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Version in the Original DB: ." 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct." 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
COMMENT                          1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  kanMX" 1))) "Standard Fields") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 3 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "REFERENCE   1  (bases 1 to 6144)" 1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  AUTHORS   ." 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  TITLE     Direct Submission" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  JOURNAL   Exported 7. feb 2014 from SnapGene 2.2.1" 
COMMENT                          1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "            http://www.snapgene.com" 1))) 
COMMENT                   "References") 
COMMENT              #51=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Ghent University" 1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Technologiepark 927" 1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "B-9052 Gent-Zwijnaarde Belgium" 1) 
COMMENT                     #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
COMMENT                     #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
COMMENT                          1) 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #59=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #60=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #61=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #62=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 9501") 
COMMENT                     #63=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #64=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 28-MAY-2019"))) 
COMMENT                   "Annotations") 
COMMENT              #65=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 1 (3 #5# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 861  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(6143..859)" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1))) 
COMMENT                                 "ampicillin resistance") 
COMMENT                            #73=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2786 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2786  End: 3595" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2784..3593" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /transl_table=11" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"kanMX\"" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"geneticin resistance protein\"" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /db_xref=<a href=\"ncbi:sequence/11344910\">GI:11344910</a>" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /protein_id=<a href=\"ncbi:protein/AAG34543.1\">AAG34543.1</a>" 
COMMENT                                        1))) "Tn903 neomycin resistance"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #84=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 2 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1246 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1246  End: 1279" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1244..1277" 1))) "FRT") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1320 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1320  End: 1855" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1318..1852" 1))) 
COMMENT                                 "S. cerevisiae chrom. XII integration targeting sequence") 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2093 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2093  End: 2116" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2091..2114" 1))) 
COMMENT                                 "USER cloning cassette") 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2354 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2354  End: 2387 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(2352..2385)" 1))) "loxP") 
COMMENT                            #101=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3861 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3861  End: 3894 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #103=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #104=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(3859..3892)" 1))) 
COMMENT                                  "loxP") 
COMMENT                            #105=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4039 (3 #21# 0) 1 2 2 1 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4039  End: 4602" 1) 
COMMENT                                    #107=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #108=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   4041..4599" 1))) 
COMMENT                                  "S. cerevisiae chrom. XII integration targeting sequence") 
COMMENT                            #109=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 4644 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #110=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 4644  End: 4677 (Complementary)" 
COMMENT                                          1) 
COMMENT                                    #111=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Original Location Description:" 1) 
COMMENT                                    #112=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   complement(4642..4675)" 1))) "FRT"))) 
COMMENT                          "Misc. Feature (7 total)") 
COMMENT                     #113=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #114=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2442 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2442  End: 2785" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2405..2783" 1))) 
COMMENT                                   "E. gossypii TEF1 promoter"))) 
COMMENT                           "Promoter Eukaryotic (1 total)") 
COMMENT                     #118=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #119=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3940 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3940  End: 3956 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3938..3954)" 1))) 
COMMENT                                   "T7 promoter") 
COMMENT                             #123=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4678 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4678  End: 4694 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4676..4690)" 1))) 
COMMENT                                   "T7 promoter"))) 
COMMENT                           "Promoter Prokaryotic (2 total)") 
COMMENT                     #127=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #128=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5250 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5250  End: 5651 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5250..5649)" 1))) 
COMMENT                                   "pMB1 ori"))) "Replication Origin (1 total)") 
COMMENT                     #132=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #133=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1165 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1165  End: 1202 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #136=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1163..1200)" 1))) 
COMMENT                                   "T1 terminator") 
COMMENT                             #137=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1957 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1957  End: 2058 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1955..2056)" 1))) 
COMMENT                                   "S. cerevisiae ADH1 terminator") 
COMMENT                             #141=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2116 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2116  End: 2306" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #144=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2114..2304" 1))) 
COMMENT                                   "S. cerevisiae CYC1 terminator") 
COMMENT                             #145=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3598 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #146=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3598  End: 3798" 1) 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3596..3828" 1))) 
COMMENT                                   "E. gossypii TEF1 terminator"))) 
COMMENT                           "Terminator (4 total)"))) "Feature Map") 
COMMENT              #149=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #150=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #151=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2786 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #152=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 2786  End: 3595" 1) 
COMMENT                                      #153=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "kanMX" 1) 
COMMENT                                      #154=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #155=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   2784..3593" 1) 
COMMENT                                      #156=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #157=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /gene=\"kanMX\"" 1))) "kanMX") 
COMMENT                              #158=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3194 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #159=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 3194  End: 3217" 1) 
COMMENT                                      #160=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "kanMX" 1) 
COMMENT                                      #161=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #162=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   3192..3215" 1) 
COMMENT                                      #163=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #164=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /gene=\"kanMX\"" 1))) "kanMX"))) 
COMMENT                            "Gene (2 total)") 
COMMENT                      #165=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #166=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2314 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #167=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 2314  End: 3926" 1) 
COMMENT                                      #168=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "derived from Saccharomyces cerevisiae" 
COMMENT                                            1) 
COMMENT                                      #169=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #170=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   2312..3924" 1) 
COMMENT                                      #171=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #172=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"PCR template vector pUG6\"" 
COMMENT                                            1) 
COMMENT                                      #173=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #174=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /db_xref=\"taxon:142962\"" 1))) 
COMMENT                                    "Source_2") 
COMMENT                              #175=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3194 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #176=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 3194  End: 3217" 1) 
COMMENT                                      #177=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "derived from Saccharomyces cerevisiae" 
COMMENT                                            1) 
COMMENT                                      #178=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #179=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   3192..3215" 1) 
COMMENT                                      #180=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #181=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"PCR template vector pUG6\"" 
COMMENT                                            1) 
COMMENT                                      #182=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1) 
COMMENT                                      #183=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /db_xref=\"taxon:142962\"" 1))) 
COMMENT                                    "Source_3") 
COMMENT                              #184=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 6082 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #185=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 6082  End: 6081" 1) 
COMMENT                                      #186=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #187=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   6080..6079" 1) 
COMMENT                                      #188=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #189=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #190=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1))) 
COMMENT                                    "Source_1"))) "Source (3 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
COMMENT              #191=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #192=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #193=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #194=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2398 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #195=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #196=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #197=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2785 " 1))))) "NcoI: 1 site") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #198=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #199=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #200=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3846 " 1))))) "XhoI: 1 site") 
COMMENT                            1 5 "CTCGAG" "GAGCTC") 
COMMENT                      #201=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #202=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #203=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2321 " 1) 
COMMENT                                      #204=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3330 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #205=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 5 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #206=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #207=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 879 " 1) 
COMMENT                                      #208=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1735 " 1) 
COMMENT                                      #209=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3137 " 1) 
COMMENT                                      #210=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4863 " 1) 
COMMENT                                      #211=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5024 " 1))))) "SspI: 5 sites") 
COMMENT                            3 3 "AATATT" "TTATAA"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #212=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #213=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #214=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #215=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #216=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #217=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #218=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #219=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #220=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #221=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #222=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #223=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #224=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #225=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #226=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #227=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #228=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #229=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #230=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #231=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #232=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #233=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #234=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #235=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #236=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #237=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #238=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #239=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #240=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #241=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #242=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #243=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #244=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #245=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #246=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #247=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #248=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #249=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #250=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #251=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #252=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #253=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #254=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #255=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #256=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #257=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #258=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #259=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #260=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #261=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #262=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #263=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #264=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #265=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #266=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #267=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #268=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #269=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #270=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #271=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 70.2726 70.2726 39 39 0.1 
COMMENT         -6144) 1 1 0 1 1 
COMMENT        (mapper: 36.3507 -13.2699 70.2726 70.2726 0.01 10 -1 6144 6144 1 0 0) 
COMMENT        #272=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -116 100) 
COMMENT              (CObjectList 
COMMENT               #273=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -900 100) 
COMMENT                     (CObjectList 
COMMENT                      #274=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #218# 101859300 0) 
COMMENT                             (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27521 
COMMENT                             6.27321 1 0.0214037) 0.102103) 
COMMENT                      #275=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #215# 1096045635 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 111 42 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "pCfB2225" "@N" 1 0.0996121 2.4365 0 
COMMENT                            -10 6.21864 1.56533 Nil) 
COMMENT                      #276=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #214# 89164884 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 127 48 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "6144 bp" "@L bp" 16 
COMMENT                            0.0996121 0.551865 0 -10.8 5.15137 1.75033 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #277=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -900 100) 
COMMENT                     (CObjectList 
COMMENT                      #278=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -514 100) 
COMMENT                            (CObjectList 
COMMENT                             #279=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #216# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.39408 6.27319 1 0.082322) 0.8 1.8 1) 
COMMENT                             #280=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# -16777216 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ampicillin resistance" "@N" 1 10.5186 11.692 
COMMENT                                   0 -10 9.03624 1.13842 #279#) 
COMMENT                             #281=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #216# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 2.6026 
COMMENT                                    3.42963 1 0.082322) 0.8 1.8 0) 
COMMENT                             #282=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# 695412850 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn903 neomycin resistance" "@N" 1 -7.79253 
COMMENT                                   -14.7578 0 -10 12.3519 1.13842 #281#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #283=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1861 100) 
COMMENT                            (CObjectList 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #219# 1368 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 1 3.87071 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #285=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1129596739 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   9.833 -11.0721 0 -10 2.00647 1.13842 #284#) 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #219# 
COMMENT                                    2082603128 100) (LOGPEN 0 10 16711680) 8 1 
COMMENT                                   2.33202 1.9 0.082322 1) 
COMMENT                             #287=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1094926659 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   -11.4795 -9.35378 0 -10 2.00647 1.13842 #286#) 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #219# 0 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 1 5.00201 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #289=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 544830049 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 
COMMENT                                   12.7397 3.66965 0 -10 2.00647 1.13842 #288#) 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #219# 51483752 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 1 1.53256 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #291=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "FRT" "@N" 1 
COMMENT                                   -13.2001 -0.0506875 0 -10 2.00647 1.13842 
COMMENT                                   #290#) 
COMMENT                             #292=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #219# 
COMMENT                                    2082603128 100) (LOGPEN 0 10 16711680) 8 1 
COMMENT                                   4.1372 1.9 0.082322 1) 
COMMENT                             #293=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 14746016 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "USER cloning cassette" "@N" 1 15.1209 
COMMENT                                   -6.86971 0 -10 10.317 1.13842 #292#) 
COMMENT                             #294=(CLine 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #219# 
COMMENT                                    1277180738 100) (LOGPEN 0 10 16711680) 8 
COMMENT                                   0.835356 4.37918 4.92645) 
COMMENT                             #295=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 209 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "S. cerevisiae chrom. XII integration targeting sequence" 
COMMENT                                   "@N" 1 23.4597 0 0 -10 24.4477 1.13842 #294#) 
COMMENT                             #296=(CLine 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #219# 
COMMENT                                    1734439494 100) (LOGPEN 0 10 16711680) 8 
COMMENT                                   0.835356 1.57442 2.15028) 
COMMENT                             #297=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #257# 1011 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "S. cerevisiae chrom. XII integration targeting sequence" 
COMMENT                                   "@N" 1 -23.1297 -3.87687 0 -10 24.4477 
COMMENT                                   1.13842 #296#)) (CObjectList)) 
COMMENT                      #298=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1003 100) 
COMMENT                            (CObjectList 
COMMENT                             #299=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #253# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    1.00149 3.42963 3.78086 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #300=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# 14745992 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "E. gossypii TEF1 promoter" "@N" 1 12.2822 
COMMENT                                   -14.0729 0 -10 11.925 1.13842 #299#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #301=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -391 100) 
COMMENT                            (CObjectList 
COMMENT                             #302=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #235# 0 100) 
COMMENT                                    (LOGPEN 0 8 128) 6 0.835356 2.25136 2.33863) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #303=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# 1413698627 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 -13.0213 -7.51183 0 -10 5.47866 
COMMENT                                   1.13842 #302#) 
COMMENT                             #304=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #235# 0 100) 
COMMENT                                    (LOGPEN 0 8 128) 6 0.670712 1.49785 1.58511) 
COMMENT                                   0.4 0.082322 0.750712 1.8 1.8 1) 
COMMENT                             #305=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 -14.7228 1.70764 0 -10 5.47866 1.13842 
COMMENT                                   #304#)) (CObjectList)) 
COMMENT                      #306=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1616 100) 
COMMENT                            (CObjectList 
COMMENT                             #307=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #228# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.503366 0.913818 1 0.082322) 0.8 1.8 1) 
COMMENT                             #308=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #256# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -10.0674 9.85007 0 -10 4.09833 1.13842 
COMMENT                                   #307#)) (CObjectList)) 
COMMENT                      #309=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -636 100) 
COMMENT                            (CObjectList 
COMMENT                             #310=(CLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #219# 2263 100) 
COMMENT                                   (LOGPEN 0 10 16711680) 8 0.835356 4.17191 
COMMENT                                   4.27606) 
COMMENT                             #311=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #266# 28030368 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "S. cerevisiae ADH1 terminator" "@N" 1 
COMMENT                                   17.4984 -5.56852 0 -10 13.5899 1.13842 #310#) 
COMMENT                             #312=(CLine 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #219# 543716457 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 0.835356 
COMMENT                                   3.9187 4.11371) 
COMMENT                             #313=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #266# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "S. cerevisiae CYC1 terminator" "@N" 1 
COMMENT                                   16.3232 -8.28474 0 -10 13.6753 1.13842 #312#) 
COMMENT                             #314=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #219# 544104808 
COMMENT                                    100) (LOGPEN 0 10 16711680) 8 0.835356 
COMMENT                                   2.39533 2.60055) 
COMMENT                             #315=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #266# 1827932698 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "E. gossypii TEF1 terminator" "@N" 1 -13.0383 
COMMENT                                   -11.9846 0 -10 12.4515 1.13842 #314#) 
COMMENT                             #316=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #219# 
COMMENT                                    2082604188 100) (LOGPEN 0 10 16711680) 8 1 
COMMENT                                   5.08471 1.9 0.082322 1) 
COMMENT                             #317=(CLabel (CWidget 0 (0 0) 1 2 0 0 #266# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 81 30 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T1 terminator" 
COMMENT                                   "@N" 1 14.2461 4.99931 0 -10 5.99096 1.13842 
COMMENT                                   #316#)) (CObjectList))) (CObjectList)) 
COMMENT               #318=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1616 100) 
COMMENT                     (CObjectList 
COMMENT                      #319=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1371 100) 
COMMENT                            (CObjectList 
COMMENT                             #320=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #271# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 3.82579 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #321=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #268# 879 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bgl}II (2398)" "@N (@S)" 5 10.9657 
COMMENT                                   -12.601 0 -10 4.86676 1.03881 #320#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #322=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1861 100) 
COMMENT                            (CObjectList 
COMMENT                             #323=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #271# 2060184304 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 3.43065 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #324=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #268# 1953718126 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Nco}I (2785)" "@N (@S)" 5 7.03445 
COMMENT                                   -15.6303 0 -10 4.78138 1.03881 #323#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #325=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil 1919957609 100) 
COMMENT                            (CObjectList 
COMMENT                             #326=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #271# 98250184 
COMMENT                                    100) (LOGPEN 0 0 16724736) 1 1 2.34734 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #327=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #268# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Xho}I (3846)" "@N (@S)" 5 -11.7667 
COMMENT                                   -10.6692 0 -10 4.78138 1.03881 #326#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #328=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -2105 100) 
COMMENT                            (CObjectList 
COMMENT                             #329=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #270# 1128482644 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 3.9044 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #330=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #269# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (2321)" "@N (@S)" 5 12.0721 
COMMENT                                   -9.7567 0 -10 5.72058 1.03881 #329#) 
COMMENT                             #331=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #270# 2082603128 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 2.87419 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #332=(CLabel (CWidget 0 (0 0) 1 2 0 0 #269# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (3330)" "@N (@S)" 5 -6.40938 
COMMENT                                   -13.3997 0 -10 5.72058 1.03881 #331#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #333=(CGroupWidget 
COMMENT                            (CWidget 5 (10 #3# 0) 1 2 0 0 Nil -518 100) 
COMMENT                            (CObjectList 
COMMENT                             #334=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #270# 2082603128 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 5.37672 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #335=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #269# 1634140336 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Ssp}I (879)" "@N (@S)" 5 11.4136 
COMMENT                                   7.92275 0 -10 4.09833 1.03881 #334#) 
COMMENT                             #336=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #270# 2082603128 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 4.50273 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #337=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #269# 265 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Ssp}I (1735)" "@N (@S)" 5 13.8963 
COMMENT                                   -3.02306 0 -10 4.58216 1.03881 #336#) 
COMMENT                             #338=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #270# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.07125 1.9 0.082322 1) 
COMMENT                             #339=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #269# 1129595719 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Ssp}I (3137)" "@N (@S)" 5 -3.31381 
COMMENT                                   -16.1159 0 -10 4.58216 1.03881 #338#) 
COMMENT                             #340=(CScratch 
COMMENT                                   (CWidget 4 (1 #3# 4) 1 2 0 0 #270# 98251304 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 1.30896 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #341=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #269# 1184741065 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Ssp}I (4863)" "@N (@S)" 5 -13.5882 
COMMENT                                   3.66965 0 -10 4.58216 1.03881 #340#) 
COMMENT                             #342=(CScratch 
COMMENT                                   (CWidget 5 (1 #3# 5) 1 2 0 0 #270# 99327032 
COMMENT                                    100) (LOGPEN 0 0 39321) 1 1 1.14457 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #343=(CLabel (CWidget 0 (0 0) 1 2 0 0 #269# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Ssp}I (5024)" "@N (@S)" 5 -12.7511 
COMMENT                                   5.22699 0 -10 4.58216 1.03881 #342#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #344=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1768055662 100) 
COMMENT                     (CObjectList 
COMMENT                      #345=(CLabel 
COMMENT                            (CWidget 0 (15 #22# 0) 1 2 0 0 #241# 221327413 100) 
COMMENT                            (LOGPEN 1128481312 1699163981 1919250034) 1634488352 
COMMENT                            (LOGFONT 111 42 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 9501" 
COMMENT                            "LMBP 9501" 65535 0.184994 -1.23804 0 -10 7.11515 
COMMENT                            1.56533 Nil) 
COMMENT                      #346=(CLabel 
COMMENT                            (CWidget 0 (15 #23# 0) 1 2 0 0 #241# 221327413 100) 
COMMENT                            (LOGPEN 1128481312 1699163981 1919250034) 1634488352 
COMMENT                            (LOGFONT 74 28 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 28-MAY-2019" 
COMMENT                            "Last modified: 28-MAY-2019" 65535 0.327297 -2.88875 
COMMENT                            0 -10 11.6119 1.03881 Nil)) (CObjectList)) 
COMMENT               #347=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1768055662 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 86969216 
COMMENT       (CStringList) 86269568 86586776 (CObList)))
FEATURES             Location/Qualifiers
     source          6082..6081
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     source          2314..3926
                     /organism="PCR template vector pUG6"
                     /mol_type="other DNA"
                     /db_xref="taxon:142962"
                     /vntifkey="98"
                     /note="derived from Saccharomyces cerevisiae"
     source          3194..3217
                     /organism="PCR template vector pUG6"
                     /mol_type="other DNA"
                     /db_xref="taxon:142962"
                     /vntifkey="98"
                     /note="derived from Saccharomyces cerevisiae"
     CDS             complement(1..861)
                     /codon_start=1
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    1320..1855
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence
     terminator      complement(1957..2058)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2116..2306
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    complement(2354..2387)
                     /vntifkey="21"
                     /label=loxP
     promoter        2442..2785
                     /vntifkey="29"
                     /label=E.\gossypii\TEF1\promoter
     gene            2786..3595
                     /gene="kanMX"
                     /vntifkey="60"
                     /note="kanMX"
     CDS             2786..3595
                     /codon_start=1
                     /transl_table=11
                     /gene="kanMX"
                     /product="geneticin resistance protein"
                     /db_xref="GI:11344910"
                     /protein_id="AAG34543.1"
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     gene            3194..3217
                     /gene="kanMX"
                     /vntifkey="60"
                     /note="kanMX"
     terminator      3598..3798
                     /vntifkey="43"
                     /label=E.\gossypii\TEF1\terminator
     misc_feature    complement(3861..3894)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    4039..4602
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence
     terminator      complement(1165..1202)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1246..1279
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4644..4677)
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2093..2116
                     /vntifkey="21"
                     /label=USER\cloning\cassette
     promoter        complement(3940..3956)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4678..4694)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(5250..5651)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1665 a      1387 c      1320 g      1772 t 
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
      121 cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa 
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
      301 cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
      841 acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca tttatcaggg 
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
      961 cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta tacctgaata 
     1021 tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 
     1081 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact ccccatgcga 
     1141 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 
     1201 cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt tcctattctc 
     1261 tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga gggtttaatc 
     1321 ggcatgcaaa catctacaca attagcaagg gcaatccata ttttgtcttt tcgcgccctg 
     1381 gaaaggccta agtaatgtcg taaacgcatt ctatctgtac ttcaactctc ctctgtgcat 
     1441 tggtttgtgc aaatcacatt ttacgatact gccagatata tgcaaaaaga gaaaaccaag 
     1501 ggaccagaac aaagcaaaat tacgatattc ttcgaattcc ttcgtgcttg actaagacaa 
     1561 agggatggac gtagcgattt ttagcgggcc aagaactggt tccgaaaaag cacaggtaca 
     1621 ccgaaccctc agctaaggag ggacagcacc gatgcggaag gacaaacttt ctttttgcct 
     1681 atcacagtat cttatcgagc taactatttt cgacacacat gaaaaagcag aaatattaac 
     1741 gaaaaagaaa agaaagacca tgtcatgtac gggcaatcag aatctgtaac aagcgccatt 
     1801 tttttttctg tatcgggccc tccttactgc tctccttccg tgtaacgcgt tatgattaag 
     1861 tcctcagcga gctcgcatgg aatgcgtgcg atgagcgacc tcatgctata cctgagaaag 
     1921 caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga 
     1981 gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat 
     2041 aaatcataag aaattcgctt atttagaagt gtcaacaacg tatctaccaa cggaatgcgt 
     2101 gcgatcgcgt gcattcatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct 
     2161 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa 
     2221 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc 
     2281 ttgagaaggt tttgggacgc tcgaagatcg cgtcagctga agcttcgtac gctgcaggtc 
     2341 gacaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctagagat 
     2401 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata 
     2461 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca 
     2521 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg 
     2581 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca 
     2641 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt 
     2701 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc 
     2761 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg 
     2821 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 
     2881 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct 
     2941 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 
     3001 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 
     3061 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc 
     3121 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tacattcgat 
     3181 tcctgtttgt aattgtcctt ttaacagcga ccgtgtattt cgtctcgctc aggcgcaatc 
     3241 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 
     3301 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt 
     3361 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 
     3421 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 
     3481 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 
     3541 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt 
     3601 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt 
     3661 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc 
     3721 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga 
     3781 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt gtcgaaaacg 
     3841 agctctcgag aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtg 
     3901 atatcagatc cactagtggc ctatgcaccc aattcgccct atagtgagtc gtattacgcg 
     3961 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccctgca 
     4021 ggactagtgc tgaggcatta atctactatc ggcgactctc tcgaaatttt tcttaacgcg 
     4081 tccttgtact gcgtctaacg cttttgccac ttggatttct attataggaa atagtctcac 
     4141 ttactgggcg acgaattttc gcgttttgat gaagcacagg aagaatttct tttttttttg 
     4201 gcttcttctg gttccgtttt ttacgcgcac aaatctaaaa aaagaaataa ttataaccta 
     4261 gtctcgaaaa ttttcatcga tccattcgtt cctttttttc gattttttca gatcaaaatt 
     4321 cttgtttctt tctttgtctt agtttatatt aaaagatatt ttgattttac tcctgaacta 
     4381 tttattcttt ctaagaaggc cagaacacta cagctgtttt aaccgactac gaagttctcc 
     4441 attctcgaac actagccttc atttaccaaa caggaactag cgtatatcat tagtccttat 
     4501 tcgaaaagag attggtagat atttattgta gtttgtgaga aggagaaaat actgtcattg 
     4561 gactgatagt tagaggacat taacctctct tacgttcgct cattaagacc tcagcgcggc 
     4621 cgcaaattta aataaaatga agtgaagttc ctatactttc tagagaatag gaacttctat 
     4681 agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat actgataaca 
     4741 tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa 
     4801 ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa 
     4861 atattgtata tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc 
     4921 aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta 
     4981 aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct 
     5041 ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat 
     5101 taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg gccgtcgttt 
     5161 tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg ccttctgctt 
     5221 agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac tgactcgctg 
     5281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     5341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     5401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     5461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     5521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     5581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     5641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     5701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     5761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     5821 ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta 
     5881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     5941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     6001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     6061 tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg cgccgtcccg 
     6121 tcaagtcagc gtaatgctct gctt 
//
