ID pCfB2225 standard; circular DNA; SYN; 6144 BP. AC .; DT 20-MAR-2015 DE LMBP 9501. KW p0120 pXII-2-kanMXsyn. OS synthetic DNA construct CC Accession number: LMBP 9501 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 28-MAY-2019 CC CC GBRFLN|REFERENCE 1 (bases 1 to 6144) CC GBRFLN| AUTHORS . CC GBRFLN| TITLE Direct Submission CC GBRFLN| JOURNAL Exported 7. feb 2014 from SnapGene 2.2.1 CC GBRFLN| http://www.snapgene.com CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|728913804| CC VNTDBDATE|-12834228| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid CC VNTEXTCHREPL|Bacteria FH Key Location/Qualifiers FH FT source 6082..6081 FT /organism="synthetic DNA construct" FT /mol_type="other DNA" FT /vntifkey="98" FT source 2314..3926 FT /organism="PCR template vector pUG6" FT /mol_type="other DNA" FT /db_xref="taxon:142962" FT /vntifkey="98" FT /note="derived from Saccharomyces cerevisiae" FT source 3194..3217 FT /organism="PCR template vector pUG6" FT /mol_type="other DNA" FT /db_xref="taxon:142962" FT /vntifkey="98" FT /note="derived from Saccharomyces cerevisiae" FT CDS complement(1..861) FT /codon_start=1 FT /vntifkey="4" FT /label=ampicillin\resistance FT misc_feature 1320..1855 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence FT terminator complement(1957..2058) FT /vntifkey="43" FT /label=S.\cerevisiae\ADH1\terminator FT terminator 2116..2306 FT /vntifkey="43" FT /label=S.\cerevisiae\CYC1\terminator FT misc_feature complement(2354..2387) FT /vntifkey="21" FT /label=loxP FT promoter 2442..2785 FT /vntifkey="29" FT /label=E.\gossypii\TEF1\promoter FT gene 2786..3595 FT /gene="kanMX" FT /vntifkey="60" FT /note="kanMX" FT CDS 2786..3595 FT /codon_start=1 FT /transl_table=11 FT /gene="kanMX" FT /product="geneticin resistance protein" FT /db_xref="GI:11344910" FT /protein_id="AAG34543.1" FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT gene 3194..3217 FT /gene="kanMX" FT /vntifkey="60" FT /note="kanMX" FT terminator 3598..3798 FT /vntifkey="43" FT /label=E.\gossypii\TEF1\terminator FT misc_feature complement(3861..3894) FT /vntifkey="21" FT /label=loxP FT misc_feature 4039..4602 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XII\integration\targeting\sequence FT terminator complement(1165..1202) FT /vntifkey="43" FT /label=T1\terminator FT misc_feature 1246..1279 FT /vntifkey="21" FT /label=FRT FT misc_feature complement(4644..4677) FT /vntifkey="21" FT /label=FRT FT misc_feature 2093..2116 FT /vntifkey="21" FT /label=USER\cloning\cassette FT promoter complement(3940..3956) FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(4678..4694) FT /vntifkey="30" FT /label=T7\promoter FT rep_origin complement(5250..5651) FT /vntifkey="33" FT /label=pMB1\ori SQ Sequence 6144 BP; 1665 A; 1387 C; 1320 G; 1772 t; ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 60 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 120 cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa 180 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 240 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 300 cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 360 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 420 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 480 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 540 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 600 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 660 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 720 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 780 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 840 acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca tttatcaggg 900 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 960 cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta tacctgaata 1020 tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 1080 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact ccccatgcga 1140 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1200 cgcccgggct aattatgggg tgtcgccctt attcgactct atagtgaagt tcctattctc 1260 tagaaagtat aggaacttct gaagtgggga tttaaatgcg gccgcgctga gggtttaatc 1320 ggcatgcaaa catctacaca attagcaagg gcaatccata ttttgtcttt tcgcgccctg 1380 gaaaggccta agtaatgtcg taaacgcatt ctatctgtac ttcaactctc ctctgtgcat 1440 tggtttgtgc aaatcacatt ttacgatact gccagatata tgcaaaaaga gaaaaccaag 1500 ggaccagaac aaagcaaaat tacgatattc ttcgaattcc ttcgtgcttg actaagacaa 1560 agggatggac gtagcgattt ttagcgggcc aagaactggt tccgaaaaag cacaggtaca 1620 ccgaaccctc agctaaggag ggacagcacc gatgcggaag gacaaacttt ctttttgcct 1680 atcacagtat cttatcgagc taactatttt cgacacacat gaaaaagcag aaatattaac 1740 gaaaaagaaa agaaagacca tgtcatgtac gggcaatcag aatctgtaac aagcgccatt 1800 tttttttctg tatcgggccc tccttactgc tctccttccg tgtaacgcgt tatgattaag 1860 tcctcagcga gctcgcatgg aatgcgtgcg atgagcgacc tcatgctata cctgagaaag 1920 caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta aaacctaaga 1980 gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat 2040 aaatcataag aaattcgctt atttagaagt gtcaacaacg tatctaccaa cggaatgcgt 2100 gcgatcgcgt gcattcatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct 2160 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa 2220 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc 2280 ttgagaaggt tttgggacgc tcgaagatcg cgtcagctga agcttcgtac gctgcaggtc 2340 gacaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtctagagat 2400 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata 2460 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca 2520 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg 2580 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca 2640 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt 2700 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc 2760 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg 2820 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 2880 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct 2940 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 3000 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 3060 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc 3120 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tacattcgat 3180 tcctgtttgt aattgtcctt ttaacagcga ccgtgtattt cgtctcgctc aggcgcaatc 3240 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 3300 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt 3360 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 3420 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 3480 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 3540 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt 3600 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt 3660 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc 3720 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga 3780 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt gtcgaaaacg 3840 agctctcgag aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtg 3900 atatcagatc cactagtggc ctatgcaccc aattcgccct atagtgagtc gtattacgcg 3960 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccctgca 4020 ggactagtgc tgaggcatta atctactatc ggcgactctc tcgaaatttt tcttaacgcg 4080 tccttgtact gcgtctaacg cttttgccac ttggatttct attataggaa atagtctcac 4140 ttactgggcg acgaattttc gcgttttgat gaagcacagg aagaatttct tttttttttg 4200 gcttcttctg gttccgtttt ttacgcgcac aaatctaaaa aaagaaataa ttataaccta 4260 gtctcgaaaa ttttcatcga tccattcgtt cctttttttc gattttttca gatcaaaatt 4320 cttgtttctt tctttgtctt agtttatatt aaaagatatt ttgattttac tcctgaacta 4380 tttattcttt ctaagaaggc cagaacacta cagctgtttt aaccgactac gaagttctcc 4440 attctcgaac actagccttc atttaccaaa caggaactag cgtatatcat tagtccttat 4500 tcgaaaagag attggtagat atttattgta gtttgtgaga aggagaaaat actgtcattg 4560 gactgatagt tagaggacat taacctctct tacgttcgct cattaagacc tcagcgcggc 4620 cgcaaattta aataaaatga agtgaagttc ctatactttc tagagaatag gaacttctat 4680 agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat actgataaca 4740 tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa 4800 ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa 4860 atattgtata tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc 4920 aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta 4980 aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct 5040 ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat 5100 taacgattac tcgttatcag aaccgcccag ggggcccgag cttaagactg gccgtcgttt 5160 tacaacacag aaagagtttg tagaaacgca aaaaggccat ccgtcagggg ccttctgctt 5220 agtttgatgc ctggcagttc cctactctcg ccttccgctt cctcgctcac tgactcgctg 5280 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 5340 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 5400 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 5460 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 5520 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5580 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5640 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5700 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5760 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5820 ggcggtgcta cagagttctt gaagtggtgg gctaactacg gctacactag aagaacagta 5880 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5940 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 6000 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 6060 tggaacgacg cgcgcgtaac tcacgttaag ggattttggt catgagcttg cgccgtcccg 6120 tcaagtcagc gtaatgctct gctt 6144 //