LOCUS pCfB388 6964 bp DNA circular UNA 20-MAR-2015 DEFINITION LMBP 9502. ACCESSION BGHA URA3 ADH1 CYC1 Nb.BsmI SOURCE Saccharomyces cerevisiae (unknown). ORGANISM Saccharomyces cerevisiae Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. COMMENT Accession number: LMBP 9502 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 20-MAR-2015 COMMENT VNTDATE|728914623| COMMENT VNTNAME|pCfB388| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"pCfB388 (pXI-1-LoxP-KlLEU2)" 535 877 "SspI" 1412 "SspI" COMMENT (LOGPEN 0 0 0) 1.21793e-316 4.67589e-283) COMMENT #294=(CSampFrag "pCfB388 (pXI-1-LoxP-KlLEU2)" 161 5681 "SspI" 5842 COMMENT "SspI" (LOGPEN 0 0 0) 3.95063e-283 0)))) FEATURES Location/Qualifiers gene complement(1..859) /vntifkey="60" misc_feature 1318..1787 /vntifkey="21" /label=S.\cerevisiae\chrom.\XI\fragment terminator complement(1890..1994) /vntifkey="43" /label=S.\cerevisiae\ADH1\terminator terminator 2049..2239 /vntifkey="43" /label=S.\cerevisiae\CYC1\terminator misc_feature 4795..5419 /vntifkey="21" /label=S.\cerevisiae\chrom.\XI\fragment CDS complement(6963..859) /vntifkey="4" /label=ampicillin\resistance misc_feature complement(2287..2320) /vntifkey="21" /label=loxP CDS complement(2474..3562) /gene="LEU2" /codon_start=1 /transl_table=11 /product="beta-isopropylmalate dehydrogenase" /protein_id="AAG34541.1" /db_xref="GI:11344907" /vntifkey="4" /label=K.\lactis\LEU2 misc_feature complement(4609..4642) /vntifkey="21" /label=loxP terminator complement(2336..2473) /vntifkey="43" /label=K.\lactis\LEU2\terminator promoter complement(3563..4587) /vntifkey="29" /label=K.\lactis\LEU2\promoter terminator complement(1163..1200) /vntifkey="43" /label=T1\terminator misc_feature 1244..1277 /vntifkey="21" /label=FRT misc_feature complement(5462..5495) /vntifkey="21" /label=FRT misc_feature 2026..2049 /vntifkey="21" /label=USER promoter complement(4692..4708) /vntifkey="30" /label=T7\promoter promoter complement(5496..5512) /vntifkey="30" /label=T7\promoter rep_origin complement(6070..6469) /vntifkey="33" /label=pMB1\ori BASE COUNT 1941 a 1481 c 1593 g 1949 t ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgga 1321 atagtgacgt tgtgatgcgg tgagttcggc ggttagggga atggtatatg ataaaaaacg 1381 gaaacgtgct tctttaattt aattgtttaa tattgttgca gatatataaa aagggggaaa 1441 gaaccaaaga tgtaattatt tctttattgc ctcaacctaa agcaagcaat aaggtataga 1501 gatcaggacg tctcgagagc tgatatcaaa tttgaagcca cgcaagtaac tacgtaggtc 1561 agagggcaca aggaataaca cgtgacattt ttcttttttc tttttttttt tttttttttt 1621 ttttgttagt cttggcttct gtgccgtagt ctgtatacgg ttttagatgc ggtatgttta 1681 tcatcgccca gaaatttgcg gggtgcaaag aaataaaatc cgtgctgaaa cccgtgctga 1741 aatccgtgca ccgcatcaaa ttttctcgga ggattctttg cagccggatt aagtcctcag 1801 cgagctcgca tggaatgcgt gcgatgagcg acctcatgct atacctgaga aagcaacctg 1861 acctacagga aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt 1921 taaaatttgt atacacttat tttttttata acttatttaa taataaaaat cataaatcat 1981 aagaaattcg cttatttaga agtgtcaaca acgtatctac caacggaatg cgtgcgatcg 2041 cgtgcattca tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc 2101 tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct 2161 tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa 2221 ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg tacgctgcag gtcgacaacc 2281 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga gatccgcagg 2341 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat 2401 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt 2461 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct 2521 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc 2581 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca 2641 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag 2701 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag 2761 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat 2821 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa 2881 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga 2941 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa 3001 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa 3061 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat 3121 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcgg 3181 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt 3241 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt 3301 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat 3361 cggctttctt agaggcttct agagcttcat ctggtaaagg agtgccagtg gcatcgatgg 3421 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa 3481 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta 3541 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt 3601 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat 3661 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc 3721 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt 3781 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa 3841 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcca ccacaagtac 3901 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagtctgc tatatcaaat 3961 gttaaacata actaaatgca gtagtgaata gagtaatgta tttctacata tgtgggtagg 4021 gccaatggga gcccgatgtc tattgtacag atattttcct tttatagtta atcaattcaa 4081 cttggaaatc attgagtcga tatctacttg gataactgga gccgtggcag cgtttgaaat 4141 ttgacgagtc ttcttgtttt gttcgcgcat tttcagttta ttcaaacggg agatggcacc 4201 gaatccactg acttctctgt tcaatttgaa tggtaatagg aagaaagtac atttcgagaa 4261 attttcgccc aacgtcgaaa tctcatggat gatatcctcg atacaaatga caccttcatc 4321 acctaatttc tcttcaacga tattgttgtc attcaagatt atctcttttg gttctttatc 4381 ttctggcctt tgccacatta ttctagatct cttttggatt aaagaacgga tagatgcaag 4441 tgaaggttgt ccgataacaa tatatggtga tacaagtttc aacaatgggt acacgtcctt 4501 ggtaagtttg atgaaaacac cagtgttcaa ctcaacaagt ctcaaaagag ataacacttt 4561 gtaagccttt gctgggatct tcacagcgag ctctcgagaa cccttaatat aacttcgtat 4621 aatgtatgct atacgaagtt attaggtagg tgatatcaga tccactagtg gcctatgcac 4681 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 4741 gtgactggga aaaccctggc gttacccctg caggactagt gctgaggcat taatcttcca 4801 cggaatacca agcccattgc atcgcgatgt tagtttagcg gagtttcttg gcattggcaa 4861 atctctgcta aatgctgcgt acagacggaa actcacaccg ccgcgaagac tggtcagtgg 4921 caaaaaaaaa ataaaaatat agaaaataac tattacgtat gttactgttt ctggtagttg 4981 atatgaagtt ggagttgtat attgtacgct ttaggaacag ggaagtgaat attatttact 5041 ctgctgcaca ttctggctag gtcgaagccg gaacttgaga agacgccgcg ctagaactat 5101 ggaccaagct gttgacaatg ttcagatggt gatgcaccta ccctgtgcgg ggagtggcca 5161 cggacgcgag cggaaggtgc ggaaggtgcg gaaggtgcgg gagttgcggg aggttcttcg 5221 ctaagcgtga gggttgctag ctggggcggc ggggtttccc taagtgtaaa taaggcctcg 5281 gctggcacat gagtcgccgg aggaggcggc ggaggcgacg acgctaaaac cgtggccgtt 5341 ggggaaggat gggcggctat atctaccatt gacctgatgg ggactcggtt cttaaggaat 5401 gggtttgagg tgggtgtgga ttaagacctc agcgcggccg caaatttaaa taaaatgaag 5461 tgaagttcct atactttcta gagaatagga acttctatag tgagtcgaat aagggcgaca 5521 caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt 5581 tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt 5641 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca 5701 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt 5761 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca 5821 aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa 5881 aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa 5941 ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta 6001 gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc 6061 tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6241 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6661 agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 6721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 6781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc 6901 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc 6961 tttt //