LOCUS       pCfB389                 6161 bp    DNA     circular UNA 20-MAR-2015
DEFINITION  LMBP 9503.
ACCESSION   BGHA URA3 ADH1 CYC1 Nb.BsmI
SOURCE      Saccharomyces cerevisiae (unknown).
  ORGANISM  Saccharomyces cerevisiae
            Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
COMMENT     Accession number: LMBP 9503
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-MAR-2015
COMMENT     VNTDATE|728920072|
COMMENT     VNTNAME|pCfB389|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCfB389" 0 6161 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HaeII" "NcoI" "PvuII" "SalI" "ScaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 125 0 1110 0 2567 0 4068 
COMMENT            0 5031 0 5636 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3798 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 2349 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2371 0 2695 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 553 0 0 "")) 
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COMMENT            1886 0 (CStringList) (CStringList) 1 1 1 1 "1318..1886") 
COMMENT        #6=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 1989 2090 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2148 2338 
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COMMENT        #8=(CFSignal (CObList) "S. cerevisiae chrom. XI fragment" 21 0 0 4054 
COMMENT            4616 0 (CStringList) (CStringList) 1 1 1 1 "4054..4616") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6160 859 0 
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COMMENT             (CStringList) 1 1 1 1 "2370..2429") 
COMMENT        #11=(CFSignal (CObList) "K. lactis URA3" 4 0 1 2553 3356 0 (CStringList) 
COMMENT             (CStringList "/gene=\"URA3\"" "/codon_start=1" "/transl_table=11" 
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COMMENT              "/protein_id=\"AAG34531.1\"" "/db_xref=\"GI:11344893\"") 1 1 1 1 
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COMMENT             (CStringList) 1 1 1 1 "3856..3909") 
COMMENT        #13=(CFSignal (CObList) "K. lactis URA3 promoter" 29 0 1 3357 3852 0 
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COMMENT        #14=(CFSignal (CObList) "K. lactis URA3 terminator" 43 0 1 2435 2552 0 
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COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 5267 5666 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "T1 terminator" 43 0 1 1163 1200 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "FRT" 21 0 0 1244 1277 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "FRT" 21 0 1 4659 4692 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "USER" 21 0 0 2125 2148 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "T7 promoter" 30 0 1 3951 3967 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "T7 promoter" 30 0 1 4693 4707 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "LMBP 9503" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCfB389'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/20/15 01:47" 1) 
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COMMENT                          "Last Modified: 03/20/15 01:47" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6161 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-DEC-2013" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/BGHA\">BGHA</a>; <a href=\"ncbilink:dnarna/URA3\">URA3</a>; <a href=\"ncbilink:dnarna/ADH1\">ADH1</a>; <a href=\"ncbilink:dnarna/CYC1\">CYC1</a>; <a href=\"ncbilink:dnarna/Nb.BsmI\">Nb.BsmI</a>" 
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COMMENT                          "Sequence Source: Saccharomyces cerevisiae (unknown)." 
COMMENT                          1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/Saccharomyces+cerevisiae\">Saccharomyces cerevisiae(Saccharomyces cerevisiae)</a>" 
COMMENT                          1) 
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COMMENT                          "Taxonomy: Saccharomyces cerevisiae Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces" 
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FEATURES             Location/Qualifiers
     gene            complement(1..859)
                     /vntifkey="60"
     misc_feature    1318..1886
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XI\fragment
     terminator      complement(1989..2090)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     terminator      2148..2338
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    4054..4616
                     /vntifkey="21"
                     /label=S.\cerevisiae\chrom.\XI\fragment
     CDS             complement(6160..859)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    complement(2386..2419)
                     /vntifkey="21"
                     /label=loxP
     CDS             complement(2553..3356)
                     /gene="URA3"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="AAG34531.1"
                     /db_xref="GI:11344893"
                     /vntifkey="4"
                     /label=K.\lactis\URA3
     misc_feature    complement(3872..3905)
                     /vntifkey="21"
                     /label=loxP
     promoter        complement(3357..3852)
                     /vntifkey="29"
                     /label=K.\lactis\URA3\promoter
     terminator      complement(2435..2552)
                     /vntifkey="43"
                     /label=K.\lactis\URA3\terminator
     rep_origin      complement(5267..5666)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1163..1200)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_feature    1244..1277
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4659..4692)
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2125..2148
                     /vntifkey="21"
                     /label=USER
     promoter        complement(3951..3967)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4693..4707)
                     /vntifkey="30"
                     /label=T7\promoter
BASE COUNT     1683 a      1412 c      1286 g      1780 t 
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 
     1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgag 
     1321 gattttcgat ggagcaggat gaggagaaat agtaccacat gtatatatcc attacaaaaa 
     1381 ggtttatata caattacaat agacccttgt tggggtttct gaaaaaagaa gtagtcgatg 
     1441 ccatcggcaa taatacggaa ttacgagaaa cacaatcccg atcctttttt gggtaattac 
     1501 ttcaccgatt ctaccgattt atcatgccaa aaaaaattca ccgtgggttc tagaagtgcc 
     1561 ctttgaggat tgtagccact ctaacccaca cggcctcctt actagctgac taaggtgaca 
     1621 aaaccgcaag gactggaaag tcgccactca tctgaaaatt ctcaagtttt tcactactga 
     1681 gtttatgctt tcgaattttt ttgttcggta atagcacggc ggttcgattc aattccgccg 
     1741 ctccgagcga tgctccgcaa aactcagtaa taagctttct gatggttcac ccctttttta 
     1801 gcacgcgggg tgtaactcaa cagaaaaatg tgccatagaa caagactagg caaaagccaa 
     1861 ggagcgtttg ccatgaactt ccacaaatta agtcctcagc gagctcgcat ggaatgcgtg 
     1921 cgatgagcga cctcatgcta tacctgagaa agcaacctga cctacaggaa agagttactc 
     1981 aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt 
     2041 ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa 
     2101 gtgtcaacaa cgtatctacc aacggaatgc gtgcgatcgc gtgcattcat ccgctctaac 
     2161 cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat 
     2221 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt 
     2281 gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaagat 
     2341 cgcgtcagct gaagcttcgt acgctgcagg tcgacaaccc ttaatataac ttcgtataat 
     2401 gtatgctata cgaagttatt aggtctagag atcccaatac aacagatcac gtgatctttt 
     2461 gtaagatgaa gttgaagtga gtgttgcacc gtgccaatgc aggtggctat tagattaaat 
     2521 atgtgatttg ttctattaag tttcctgtat aattaatggg gagcgctgat tctcttttgg 
     2581 tacgcttccc atccagcatt tctgtatctt tcaccttcaa ccttaggatc tctacccttg 
     2641 gcgaaaagtc ctctgccaac aatgatgata tctgatccac cacttacaac ttcgtcgacg 
     2701 gttctgtact gctgacccaa tgcatcgcct ttgtcgtcta aacctacacc tggggtcatg 
     2761 attagccaat caaacccttc ttctcttcct cccatatcgt tctgagcaat gaacccaata 
     2821 acgaaatctt tatcactctt tgcaatatca acggtaccct tagtatattc accgtgtgct 
     2881 agagaaccct tggaagacaa ttcagcaagc atcaataatc cccttggttc tttggtgacc 
     2941 tcttgcgcac cttgtttcaa gccagcaaca ataccagcac cagtaacccc gtgggcgttg 
     3001 gtgatatcag accattctgc gatacggtaa acgcccgatg tatattgtaa tttgactgtg 
     3061 ttaccgatat cggcgaattt tctgtcctca aatatcaaga acttgtattt ctctgccaat 
     3121 gctttcaatg gaacgacagt accctcataa ctgaaatcat ccaagatatc aacgtgtgtt 
     3181 ttcaaaaggc aaatgtatgg acccaacgtt tcaacaagtt tcaatagctc atcagtcgaa 
     3241 cgaacgtcaa gagaagcaca caaattggtc ttcttttcat ccattaaacg taaaagtttc 
     3301 gatgcaaccg gacttgcatg agtctcagct ctactggtat atgattttgt ggacatggtg 
     3361 caactaattg acgggagtgt attgacgctg gcgtactggc tttcacaaaa tggcccaatc 
     3421 acaaccacat cttagatagt tgaaatgact ttagataaca tcaattgaga tgagcttaat 
     3481 catgtcaaag ctaaaagtgt caccatgaac gacaattctt aagcaaatca cgtgatatag 
     3541 atccacgaat aaccaccatt tgatgctcga ggcaagtaat gtgtgtaaaa aaatgcgtta 
     3601 ccaccatcca atgcagaccg atcttctacc cagaatcaca tatatttatg taccgagtac 
     3661 cttttttcta tcttccaatt gcttctccca tatgattgtc tccgtaagct cgaaatttct 
     3721 aagttggatt ttaatcttca cgcaggatga cagttcgatg agcttctgag gagtgtttag 
     3781 aacataatca gtttatccat ggtctatctc ttcttgtcgc tttttctcct cgatagaacc 
     3841 taaataaaac gagctctcga gaacccttaa tataacttcg tataatgtat gctatacgaa 
     3901 gttattaggt gatatcagat ccactagtgg cctatgcacc caattcgccc tatagtgagt 
     3961 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
     4021 ttacccctgc aggactagtg ctgaggcatt aatcacacga ctagcgcttt cagatattaa 
     4081 aaagtttaga tgtaggtttt agcggtaaca gttatataaa tcgtgtttct tctcttgatg 
     4141 aaacaaaaaa atgctagaaa aactttgtcg tttcttactt ttggtgcgct ttgcagtttt 
     4201 cgtggctaga cttagaatca tttctcctca gatttcttga ttaaagtttg gtgcgaagcc 
     4261 ctactctaac attggtgttc ttcttttcat tcacgcaagt taagtccagg aaggtgagca 
     4321 aatgctcatc cttctgttca tgcgtgacgg ctgaattatc cttatctggc gtacccgtgc 
     4381 agccgtttcc gtgcctcggt tcctccgaga tatccttagg gaccgccagg gaccatgatt 
     4441 gcgtcaactg ttgtcaccgc tccagaggat cctctgtaac cttttcaacc ataaaaatag 
     4501 aaagggcaca gggttggggt atttgaattt tttttttggg ttttttcgta ttacttatta 
     4561 cttaccttct tttctatata attttgtttt tccctggtag agcggaatct tcccacatta 
     4621 agacctcagc gcggccgcaa atttaaataa aatgaagtga agttcctata ctttctagag 
     4681 aataggaact tctatagtga gtcgaataag ggcgacacaa aatttattct aaatgcataa 
     4741 taaatactga taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa 
     4801 ttttgatatc aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc 
     4861 tttaacaaac tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa 
     4921 ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc 
     4981 tcatttataa ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat 
     5041 tctgacaaat gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt 
     5101 acgttatttg cggattaacg attactcgtt atcagaaccg cccagggggc ccgagcttaa 
     5161 gactggccgt cgttttacaa cacagaaaga gtttgtagaa acgcaaaaag gccatccgtc 
     5221 aggggccttc tgcttagttt gatgcctggc agttccctac tctcgccttc cgcttcctcg 
     5281 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     5341 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     5401 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     5461 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     5521 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     5581 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     5641 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     5701 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     5761 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     5821 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtgggctaa ctacggctac 
     5881 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     5941 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     6001 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     6061 gggtctgacg ctcagtggaa cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga 
     6121 gcttgcgccg tcccgtcaag tcagcgtaat gctctgcttt t 
//
