ID pCfB391 standard; circular DNA; UNA; 6065 BP. DE LMBP 9505. CC Accession number: LMBP 9505 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 1-APR-2015 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1318..1980 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XI\integration\targeting\sequence FT terminator complement(2083..2184) FT /vntifkey="43" FT /label=S.\cerevisiae\ADH1\terminator FT terminator 2242..2432 FT /vntifkey="43" FT /label=S.\cerevisiae\CYC1\terminator FT misc_feature 4010..4521 FT /vntifkey="21" FT /label=S.\cerevisiae\chrom.\XI\integration\targeting\sequence FT misc_feature complement(2480..2513) FT /vntifkey="21" FT /label=loxP FT promoter 2568..2911 FT /vntifkey="29" FT /label=E.\gossypii\TEF1\promoter FT terminator 3571..3768 FT /vntifkey="43" FT /label=E.\gossypii\TEF1\terminator FT misc_feature complement(3828..3861) FT /vntifkey="21" FT /label=loxP FT CDS complement(6064..859) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(5171..5570) FT /vntifkey="33" FT /label=pMB1\ori FT terminator complement(1163..1200) FT /vntifkey="43" FT /label=T1\terminator FT misc_feature 1244..1277 FT /vntifkey="21" FT /label=FRT FT misc_feature complement(4563..4596) FT /vntifkey="21" FT /label=FRT FT misc_feature 2214..2242 FT /vntifkey="21" FT /label=USER\cloning\cassette FT promoter complement(3907..3923) FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(4597..4611) FT /vntifkey="30" FT /label=T7\promoter FT CDS 2912..3565 FT /vntifkey="4" FT /label=S.\pombe\HIS5 SQ Sequence 6065 BP; 1677 A; 1398 C; 1294 G; 1696 t; accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 60 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 120 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 180 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 240 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 300 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 360 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 420 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 480 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 540 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 600 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 660 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 720 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 780 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 840 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 900 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 960 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 1020 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 1080 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga 1140 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1200 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta 1260 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatgcg 1320 gagaagtcgt tgatagcatt tccgaaggct tttccacatt gactgcagta tctaaaagag 1380 ctccagcagc ggtcttagta gttcctgtgc ttgccgcttt gcagtataca gggacattgg 1440 gaactacgga ggaaaaatcg gaatgctctg agctcttctc aaaaccagac gaactcgtct 1500 tatttctcag taaattaccc aacatataaa ctctccctct tcaccttctc tacaaggcca 1560 cacacaagac agttttaaca ggcaaatggt actggaggct ctcaccaatg gggtttaaga 1620 cgctccaaac acacgctctc ctttttttct ttatatatcg gtagcaacga aagctagtcg 1680 cagaacgtta cacactaaag cagatcaatc aatcaatcct gagaacctgc ttcgttcaaa 1740 ccatccacta gaagaaacac gcacgtgtac ccaaaaatag agacaaacaa acagacaaaa 1800 ataacaatga ttcctaccga ctaaagtaaa acaacactca cgtaataaaa aatcctagat 1860 acaactcact tgtattcctc tcttctttga ccatcactaa agcttctctc ttactatatt 1920 cctcaaataa aaaaaagttg caatgattac ttaccaatgt gccataaact ccgtgcacca 1980 attaagtcct cagcgagctc gcatggaatg cgtgcgatga gcgacctcat gctatacctg 2040 agaaagcaac ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac 2100 ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa 2160 aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc taccaacgga 2220 atgcgtgcga tcgcgtgcat tcatccgctc taaccgaaaa ggaaggagtt agacaacctg 2280 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat 2340 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa 2400 ccttgcttga gaaggttttg ggacgctcga agatcgcgtc agctgaagct tcgtacgctg 2460 caggtcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct 2520 agagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 2580 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 2640 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 2700 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 2760 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 2820 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 2880 gttctcacat cacatccgaa cataaacaac catgggtagg agggcttttg tagaaagaaa 2940 tacgaacgaa acgaaaatca gcgttgccat cgctttggac aaagctccct tacctgaaga 3000 gtcgaatttt attgatgaac ttataacttc caagcatgca aaccaaaagg gagaacaagt 3060 aatccaagta gacacgggaa ttggattctt ggatcacatg tatcatgcac tggctaaaca 3120 tgcaggctgg agcttacgac tttactcaag aggtgattta atcatcgatg atcatcacac 3180 tgcagaagat actgctattg cacttggtat tgcattcaag caggctatgg gtaactttgc 3240 cggcgttaaa agatttggac atgcttattg tccacttgac gaagctcttt ctagaagcgt 3300 agttgacttg tcgggacggc cctatgctgt tatcgatttg ggattaaagc gtgaaaaggt 3360 tggggaattg tcctgtgaaa tgatccctca cttactatat tccttttcgg tagcagctgg 3420 aattactttg catgttacct gcttatatgg tagtaatgac catcatcgtg ctgaaagcgc 3480 ttttaaatct ctggctgttg ccatgcgcgc ggctactagt cttactggaa gttctgaagt 3540 cccaagcacg aagggagtgt tgtaaagagt actgacaata aaaagattct tgttttcaag 3600 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc 3660 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag 3720 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga 3780 tactaacgcc gccatccagt ttaaacgagc tctcgagaac ccttaatata acttcgtata 3840 atgtatgcta tacgaagtta ttaggtgata tcagatccac tagtggccta tgcacccaat 3900 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 3960 tgggaaaacc ctggcgttac ccctgcagga ctagtgctga ggcattaatg ccttcgattt 4020 gacacatctc taagctgaaa ctgagaatac tgttgtaaaa caggtattgg ctgcttcata 4080 gtacacccaa ttgcccacaa cccaagttaa tcataagctc ttgttgtcga tgaattgctc 4140 aaaatgtggc cattctttca attttgcacc tctaaaactg ctccgtgggt tgtgacaaca 4200 accttaaggg taatggactt ttttagaaag acaaatgaga tgatttcacc tactggtata 4260 tcatttattt atacacgacc gttttgacat ttcagagttg cacgttggta tatcctaata 4320 ttctcacaag catcacagtg aagaaacgca agaaaatgga aaaaattccc aggtggttgt 4380 tgttttctct tatatcgtcg gtgctgtgca tacttggggc cctgtgtgtg ccgttgttat 4440 cggttgcctt cgatagcaag cgcaatagcc aatctaagtt ggtcaactat ggtctttctc 4500 taagtgccgg atctatgatc attaagacct cagcgcggcc gcaaatttaa ataaaatgaa 4560 gtgaagttcc tatactttct agagaatagg aacttctata gtgagtcgaa taagggcgac 4620 acaaaattta ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt 4680 ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt 4740 tcgagattta ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc 4800 ataaataata gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct 4860 tatttaaagt gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc 4920 aaagtgacag gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa 4980 aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga 5040 accgcccagg gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt 5100 agaaacgcaa aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc 5160 ctactctcgc cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 5220 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5280 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5340 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5400 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5460 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5520 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5580 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5640 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5700 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 5760 aagtggtggg ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 5820 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 5880 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 5940 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact 6000 cacgttaagg gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg 6060 ctttt 6065 //