ID pCfB132 standard; circular DNA; ; 5843 BP. DT 20-MAR-2015 DE LMBP 9506. CC Accession number: LMBP 9506 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 28-MAY-2019 CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDATE|728923277| CC VNTDBDATE|-12834281| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71| CC VNTOAUTHORAD3|B-9052 Gent Belgium| CC VNTREPLTYPE|Plasmid CC VNTREPLTYPE|Phage CC VNTEXTCHREPL|Bacteria CC VNTEXTCHREPL|Yeast FH Key Location/Qualifiers FH FT CDS 417..1220 FT /vntifkey="4" FT /label=S.\cerevisiae\URA3 FT terminator 2250..2439 FT /vntifkey="43" FT /label=S.\cerevisiae\CYC1\terminator FT CDS complement(3441..4301) FT /vntifkey="4" FT /label=ampicillin\resistance FT misc_feature 2194..2217 FT /vntifkey="21" FT /label=USER\cloning\cassette FT terminator complement(1949..2050) FT /vntifkey="43" FT /label=S.\cerevisiae\ADH1\terminator FT rep_origin complement(2498..2900) FT /vntifkey="33" FT /label=pMB1\ori FT rep_origin complement(1351..1806) FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(4433..5413) FT /vntifkey="33" FT /label=2mu\ori FT terminator complement(5491..5595) FT /vntifkey="43" FT /label=S.\cerevisiae\FLP\terminator FT misc_feature complement(5396..5443) FT /vntifkey="21" FT /label=FRT SQ Sequence 5843 BP; 1647 A; 1237 C; 1341 G; 1618 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 240 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 300 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 360 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 420 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 480 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 540 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 600 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 660 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 720 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 780 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 840 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 900 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 960 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 1020 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 1080 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 1140 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 1200 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 1260 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 1320 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 1380 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 1440 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 1500 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 1560 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 1620 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 1680 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 1740 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 1800 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 1860 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 1920 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 1980 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 2040 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 2100 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 2160 aacctctggc gaagaattgt taattaagag ctcgaatgcg tgcgatcgcg tgcattcctc 2220 gagtaagctt ggtaccgcgg ctagctaaga tccgctctaa ccgaaaagga aggagttaga 2280 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta 2340 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata 2400 ctgaaaacct tgcttgagaa ggttttggga cgctcgaaga tccagctgca ttaatgaatc 2460 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 2520 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2580 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 2640 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 2700 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 2760 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 2820 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 2880 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2940 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 3000 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 3060 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 3120 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 3180 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 3240 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3300 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 3360 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3420 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 3480 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 3540 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 3600 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 3660 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 3720 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 3780 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3840 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3900 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3960 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 4020 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 4080 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 4140 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 4200 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 4260 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 4320 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 4380 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc 4440 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa 4500 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg 4560 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa 4620 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac 4680 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt 4740 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct 4800 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt 4860 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct 4920 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg 4980 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat 5040 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta 5100 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata 5160 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag 5220 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg 5280 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt 5340 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt 5400 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 5460 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 5520 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta 5580 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc 5640 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag 5700 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc 5760 ctttgatatt ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag 5820 gcgtatcacg aggccctttc gtc 5843 //