LOCUS       pCfB291                 5918 bp    DNA     circular     20-MAR-2015
DEFINITION  LMBP 9507.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9507
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-MAR-2015
COMMENT     VNTDATE|728923295|
COMMENT     VNTNAME|pCfB291|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        #1=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 1924 0 2283 0 3961 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 4071 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 1440 0 4395 0 5387 0 0 "")) 
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COMMENT        #5=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 2326 2515 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 2025 2126 
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COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2574 2976 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3517 4377 0 
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COMMENT                          "DNA Plasmid Phage 'pCfB291'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/20/15 02:41" 1) 
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COMMENT                          "Last Modified: 03/20/15 02:41" 1) 
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FEATURES             Location/Qualifiers
     CDS             504..1163
                     /vntifkey="4"
                     /label=S.\cerevisiae\HIS3
     terminator      2326..2515
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    2270..2293
                     /vntifkey="21"
                     /label=USER
     terminator      complement(2025..2126)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     rep_origin      complement(2574..2976)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3517..4377)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(1423..1878)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(4509..5489)
                     /vntifkey="33"
                     /label=2mu\ori
     misc_feature    complement(5472..5519)
                     /vntifkey="21"
                     /label=FRT
     terminator      complement(5567..5671)
                     /vntifkey="43"
                     /label=FLP\terminator
BASE COUNT     1615 a      1301 c      1347 g      1655 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc 
     1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa 
     2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata 
     2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg 
     2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg 
     2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctcg aatgcgtgcg 
     2281 atcgcgtgca ttcctcgagt aagcttggta ccgcggctag ctaagatccg ctctaaccga 
     2341 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt 
     2401 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta 
     2461 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaagatcca 
     2521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     2581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     2641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     2701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     2761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     2821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     2881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     2941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     3001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     3061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     3121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     3181 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     3241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     3301 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     3361 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     3421 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     3481 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     3541 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     3601 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 
     3661 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 
     3721 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 
     3781 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 
     3841 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 
     3901 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 
     3961 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 
     4021 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 
     4081 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 
     4141 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 
     4201 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 
     4261 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 
     4321 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 
     4381 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 
     4441 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 
     4501 gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 
     4561 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 
     4621 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 
     4681 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 
     4741 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 
     4801 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 
     4861 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 
     4921 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 
     4981 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 
     5041 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 
     5101 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 
     5161 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 
     5221 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 
     5281 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 
     5341 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 
     5401 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 
     5461 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca 
     5521 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca 
     5581 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg 
     5641 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa 
     5701 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt 
     5761 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct 
     5821 tcaatgctat catttccttt gatattggat catctaagaa accattatta tcatgacatt 
     5881 aacctataaa aataggcgta tcacgaggcc ctttcgtc 
//
