LOCUS       pCfB220                 6970 bp    DNA     circular     20-MAR-2015
DEFINITION  LMBP 9508.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9508
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 20-MAR-2015
COMMENT     VNTDATE|728923316|
COMMENT     VNTNAME|pCfB220|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        #1=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 2975 0 3334 0 5012 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 5122 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 6 429 0 1889 0 2380 0 2491 
COMMENT            0 5446 0 6438 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 3320 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3346 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "S. cerevisiae CYC1 terminator" 43 0 0 3377 3566 
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COMMENT        #9=(CFSignal (CObList) "S. cerevisiae ADH1 terminator" 43 0 1 3076 3177 
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COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 3625 4027 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4568 5428 0 
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COMMENT                          "DNA Plasmid Phage 'pCfB220'" 1) 
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COMMENT                          "Created: 03/20/15 02:41" 1) 
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COMMENT                          "Last Modified: 03/20/15 02:41" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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FEATURES             Location/Qualifiers
     CDS             complement(663..1757)
                     /vntifkey="4"
                     /label=S.\cerevisiae\LEU2
     terminator      3377..3566
                     /vntifkey="43"
                     /label=S.\cerevisiae\CYC1\terminator
     misc_feature    3321..3344
                     /vntifkey="21"
                     /label=USER
     terminator      complement(3076..3177)
                     /vntifkey="43"
                     /label=S.\cerevisiae\ADH1\terminator
     rep_origin      complement(3625..4027)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4568..5428)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2474..2929)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(5560..6540)
                     /vntifkey="33"
                     /label=2mu\ori
     misc_feature    complement(6523..6570)
                     /vntifkey="21"
                     /label=FRT
     terminator      complement(6618..6722)
                     /vntifkey="43"
                     /label=FLP\terminator
BASE COUNT     1948 a      1497 c      1516 g      2009 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctgaatt ggagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga 
     3061 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac 
     3121 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta 
     3181 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat 
     3241 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa 
     3301 gaattgttaa ttaagagctc gaatgcgtgc gatcgcgtgc attcctcgag taagcttggt 
     3361 accgcggcta gctaagatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct 
     3421 aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa 
     3481 tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc 
     3541 ttgagaaggt tttgggacgc tcgaagatcc agctgcatta atgaatcggc caacgcgcgg 
     3601 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     3661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     3721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     4021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     4261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4561 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4621 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4681 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4741 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4801 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4861 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4921 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4981 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     5041 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     5101 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     5161 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     5221 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     5281 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     5341 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5401 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     5461 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5521 taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat 
     5581 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc 
     5641 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct 
     5701 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga 
     5761 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct 
     5821 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat 
     5881 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg 
     5941 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa 
     6001 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt 
     6061 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg 
     6121 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc 
     6181 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc 
     6241 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca 
     6301 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg 
     6361 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg 
     6421 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg 
     6481 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct 
     6541 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa 
     6601 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt 
     6661 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt 
     6721 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc 
     6781 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc 
     6841 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga 
     6901 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 
     6961 ccctttcgtc 
//
