LOCUS       pCfB1975                4117 bp    DNA     circular     15-MAY-2020
DEFINITION  LMBP 9509.
SOURCE      Cloning vector pUC19.
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     Accession number: LMBP 9509
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-MAY-2020
            NGS data implemented
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728930403|
COMMENT     VNTDBDATE|-13342180|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB1975|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     gene            complement(3057..3917)
                     /gene="bla"
                     /vntifkey="60"
     rep_origin      complement(2114..2516)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1939..2022)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             complement(3057..3917)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     RBS             complement(1901..1938)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        1418..1823
                     /vntifkey="29"
                     /label=S.\cerevisiae\TEF1\promoter
     promoter        complement(427..1244)
                     /vntifkey="29"
                     /label=S.\cerevisiae\PGK1\promoter
BASE COUNT     1117 a       946 c       937 g      1117 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccatgaca 
      421 gatttgtttt atatttgttg taaaaagtag ataattactt ccttgatgat ctgtaaaaaa 
      481 gagaaaaaga aagcatctaa gaacttgaaa aactacgaat tagaaaagac caaatatgta 
      541 tttcttgcat tgaccaattt atgcaagttt atatatatgt aaatgtaagt ttcacgaggt 
      601 tctactaaac taaaccaccc ccttggttag aagaaaagag tgtgtgagaa caggctgttg 
      661 ttgtcacacg attcggacaa ttctgtttga aagagagaga gtaacagtac gatcgaacga 
      721 actttgctct ggagatcaca gtgggcatca tagcatgtgg tactaaaccc tttcccgcca 
      781 ttccagaacc ttcgattgct tgttacaaaa cctgtgagcc gtcgctagga ccttgttgtg 
      841 tgacgaaatt ggaagctgca atcaatagga agacaggaag tcgagcgtgt ctgggttttt 
      901 tcagttttgt tctttttgca aacaaatcac gagcgacggt aatttctttc tcgataagag 
      961 gccacgtgct ttatgagggt aacatcaatt caagaaggag ggaaacactt cctttttctg 
     1021 gccctgataa tagtatgagg gtgaagccaa aataaaggat tcgcgcccaa atcggcatct 
     1081 ttaaatgcag gtatgcgata gttcctcact ctttccttac tcacgagtaa ttcttgcaaa 
     1141 tgcctattat gcagatgtta taatatctgt gcgtcttgag ttgaagtcag gaatctaaaa 
     1201 taaaaattaa ggttaataaa aagaggaaag aaaaaaaaat taatcgattt acagaaactt 
     1261 gcacactaaa aatacacaac taaaagcaat tacagtatgg gaagtcatcg acgttatctc 
     1321 tactatagta tattatcatt tctattatta tcctgctcag tggtacttgc aaaacaagat 
     1381 aagaccccat tctttgaagg tacttccagg ccggccgcac acaccatagc ttcaaaatgt 
     1441 ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc 
     1501 aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat 
     1561 tacccgtact aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg 
     1621 aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt tttttgattt 
     1681 ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca 
     1741 agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa 
     1801 gaaagcatag caatctaatc taagttttaa ttacaaagtg caggtgggga tcctctagag 
     1861 tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
     1921 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
     1981 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     2041 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     2101 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     2161 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     2221 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     2281 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     2341 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     2401 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     2461 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 
     2521 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     2581 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     2641 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     2701 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 
     2761 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     2821 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     2881 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     2941 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     3001 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     3061 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     3121 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     3181 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     3241 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     3301 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     3361 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     3421 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     3481 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     3541 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     3601 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     3661 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     3721 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     3781 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     3841 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     3901 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     3961 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     4021 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 
     4081 acctataaaa ataggcgtat cacgaggccc tttcgtc 
//
