LOCUS       pCfB1976                3702 bp    DNA     circular     15-MAY-2020
DEFINITION  LMBP 9510.
SOURCE      Cloning vector pUC19.
  ORGANISM  Cloning vector pUC19
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     Accession number: LMBP 9510
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-MAY-2020
            NGS data implemented
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728930625|
COMMENT     VNTDBDATE|-13342223|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCfB1976|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Taxonomy: other sequences; artificial sequences; vectors" 
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FEATURES             Location/Qualifiers
     gene            complement(2642..3502)
                     /gene="bla"
                     /vntifkey="60"
     CDS             complement(2642..3502)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(1699..2101)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1524..1607)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(1486..1523)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        429..547
                     /vntifkey="30"
                     /label=DAHP\synthase\promoter
     RBS             548..638
                     /vntifkey="32"
                     /label=5'\UTR\of\S1,\incl.\RBS
     CDS             639..1355
                     /vntifkey="4"
                     /label=GFP
     terminator      1356..1397
                     /vntifkey="43"
                     /label=Rho-independent\terminator
BASE COUNT      995 a       879 c       902 g       926 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accccgtgcg 
      421 atccgcatag ggagtgtaaa tttatctata cagaggtaag ggttgaaagc gcgactaaat 
      481 tgcctgtgta aataaaaatg tacgaaatat ggattgaaaa ctttacttta tgtgttatcg 
      541 ttacgtcccc cgttgcaatg gaatgacagc gggtatgtta aacaacccca tccggcatgg 
      601 agccaggtgg acgttaaata taaacctgaa gattaaacat gcgtaaagga gaagaacttt 
      661 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt 
      721 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 
      781 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg 
      841 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca 
      901 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga 
      961 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta 
     1021 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 
     1081 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta 
     1141 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa 
     1201 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt 
     1261 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg 
     1321 ggattacaca tggcatggat gaactataca aataagtgcc ggatgattca catcaccggc 
     1381 acccccgctt cggcgggctg ggcctcatgg gccttccttt cactatcgcg ggggatcctc 
     1441 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     1501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     1561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 
     1621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 
     1681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
     1741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
     1801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     1861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     1921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     1981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     2041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     2101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     2161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     2221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     2281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 
     2341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     2401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     2461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     2521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     2581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     2641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     2701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     2761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     2821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     2881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     2941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     3001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     3061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     3121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     3181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     3241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     3301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     3361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     3421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     3481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     3541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     3601 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 
     3661 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc 
//
