LOCUS pCfB1977 3832 bp DNA circular 20-MAR-2015 DEFINITION LMBP 9511. SOURCE Cloning vector pUC19. ORGANISM Cloning vector pUC19 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2686) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (18-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT Accession number: LMBP 9511 (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) Last modified: 20-MAR-2015 COMMENT VNTDATE|728930855| COMMENT VNTNAME|pCfB1977| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(LOGPEN 0 0 0) 4.52724e-316 1.02894e-314) COMMENT #205=(CSampFrag "p1977 (pUC19-PTDH3-PTEF1)" 1775 3445 "XmnI" 1388 "XmnI" COMMENT (LOGPEN 0 0 0) 1.2247e-316 1.02894e-314)))) FEATURES Location/Qualifiers gene complement(2772..3632) /gene="bla" /vntifkey="60" promoter complement(437..842) /vntifkey="29" /label=S.\cerevisiae\TEF1\promoter promoter 854..1551 /vntifkey="29" /label=S.\cerevisiae\TDH3\promoter CDS complement(2772..3632) /vntifkey="4" /label=ampicillin\resistance rep_origin complement(1829..2231) /vntifkey="33" /label=pMB1\ori promoter complement(1654..1737) /vntifkey="30" /label=lac\promoter RBS complement(1616..1653) /vntifkey="32" /label=5'\UTR\of\lacZ,\incl.\RBS BASE COUNT 1080 a 868 c 892 g 992 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccatgaca 421 gatttgtaat taaaacttag attagattgc tatgctttct ttctaatgag caagaagtaa 481 aaaaagttgt aatagaacaa gaaaaatgaa actgaaactt gagaaattga agaccgttta 541 ttaacttaaa tatcaatggg aggtcatcga aagagaaaaa aatcaaaaaa aaaaattttc 601 aagaaaaaga aacgtgataa aaatttttat tgcctttttc gacgaagaaa aagaaacgag 661 gcggtctctt ttttcttttc caaaccttta gtacgggtaa ttaacgacac cctagaggaa 721 gaaagagggg aaatttagta tgctgtgctt gggtgttttg aagtggtacg gcgatgcgcg 781 gagtccgaga aaatctggaa gagtaaaaaa ggagtagaaa cattttgaag ctatggtgtg 841 tgcatcagta gctataaaaa acacgctttt tcagttcgag tttatcatta tcaatactgc 901 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa 961 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac 1021 agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc cactaaatat 1081 aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat 1141 tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact acagagaaca 1201 ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcctggag 1261 taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc ttacaccttc 1321 tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc 1381 ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat tgattgtaat 1441 tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag 1501 ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa aagtgcaggt 1561 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg 1621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 1681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 1741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 1801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 1861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 1921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 1981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 2041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 2101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 2161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 2221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 2461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 2641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 2701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 2761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 2821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 2881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 2941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 3001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 3061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 3121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 3181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 3421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3601 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