LOCUS       pPSDzeo-FLAG-stuffer-V5-SAG1      6078 bp    DNA     circular UNA 14-MAR-2022
DEFINITION  LMBP 9523.
COMMENT     Accession number: LMBP 9523
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-MAR-2022
FEATURES             Location/Qualifiers
     promoter        44..809
                     /label=AOX1\promoter
     misc_feature    810..974
                     /label=5'\UTR\of\AOX1
     CDS             986..1228
                     /label=ppMF
     misc_feature    1253..1276
                     /label=FLAG\epitope
     CDS             1290..1532
                     /label=ppMF
     CDS             1569..2762
                     /label=hGLA
     misc_feature    2763..2792
                     /label=Myc\epitope
     misc_feature    2808..2825
                     /label=His-tag
     misc_feature    2840..2881
                     /label=V5\epitope
     CDS             2888..3850
                     /label=SAG1\fragment
     terminator      3938..4197
                     /label=AOX1\terminator
     promoter        4204..4611
                     /label=TEF1\promoter
     promoter        4619..4684
                     /label=synthetic\EM7\promoter
     CDS             4685..5059
                     /label=zeocin\resistance
     terminator      5105..5375
                     /label=CYC1\terminator
     rep_origin      complement(5385..5665)
                     /label=pMB1\ori
ORIGIN
        1 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATCAGA TCTAACATCC AAAGACGAAA
       61 GGTTGAATGA AACCTTTTTG CCATCCGACA TCCACAGGTC CATTCTCACA CATAAGTGCC
      121 AAACGCAACA GGAGGGGATA CACTAGCAGC AGACCGTTGC AAACGCAGGA CCTCCACTCC
      181 TCTTCTCCTC AACACCCACT TTTGCCATCG AAAAACCAGC CCAGTTATTG GGCTTGATTG
      241 GAGCTCGCTC ATTCCAATTC CTTCTATTAG GCTACTAACA CCATGACTTT ATTAGCCTGT
      301 CTATCCTGGC CCCCCTGGCG AGGTTCATGT TTGTTTATTT CCGAATGCAA CAAGCTCCGC
      361 ATTACACCCG AACATCACTC CAGATGAGGG CTTTCTGAGT GTGGGGTCAA ATAGTTTCAT
      421 GTTCCCCAAA TGGCCCAAAA CTGACAGTTT AAACGCTGTC TTGGAACCTA ATATGACAAA
      481 AGCGTGATCT CATCCAAGAT GAACTAAGTT TGGTTCGTTG AAATGCTAAC GGCCAGTTGG
      541 TCAAAAAGAA ACTTCCAAAA GTCGGCATAC CGTTTGTCTT GTTTGGTATT GATTGACGAA
      601 TGCTCAAAAA TAATCTCATT AATGCTTAGC GCAGTCTCTC TATCGCTTCT GAACCCCGGT
      661 GCACCTGTGC CGAAACGCAA ATGGGGAAAC ACCCGCTTTT TGGATGATTA TGCATTGTCT
      721 CCACATTGTA TGCTTCCAAG ATTCTGGTGG GAATACTGCT GATAGCCTAA CGTTCATGAT
      781 CAAAATTTAA CTGTTCTAAC CCCTACTTGA CAGCAATATA TAAACAGAAG GAAGCTGCCC
      841 TGTCTTAAAC CTTTTTTTTT ATCATCATTA TTAGCTTACT TTCATAATTG CGACTGGTTC
      901 CAATTGACAA GCTTTTGATT TTAACGACTT TTAACGACAA CTTGAGAAGA TCAAAAAACA
      961 ACTAATTATT CGAAGGATCC AAACGATGAG ATTTCCTTCA ATTTTTACTG CAGTTTTATT
     1021 CGCAGCATCC TCCGCATTAG CTGCTCCAGT CAACACTACA ACAGAAGATG AAACGGCACA
     1081 AATTCCGGCT GAAGCTGTCA TCGGTTACTC AGATTTAGAA GGGGATTTCG ATGTTGCTGT
     1141 TTTGCCATTT TCCAACAGCA CAAATAACGG GTTATTGTTT ATAAATACTA CTATTGCCAG
     1201 CATTGCTGCT AAAGAAGAAG GGGTATCTCT CGAGAAAAGA GAGGCCGAAG CGGATTACAA
     1261 AGACGATGAC GATAAGGCCG AGATGGCCGA TGAGATTTCC TTCAATTTTT ACTGCAGTTT
     1321 TATTCGCAGC ATCCTCCGCA TTAGCTGCTC CAGTCAACAC TACAACAGAA GATGAAACGG
     1381 CACAAATTCC GGCTGAAGCT GTCATCGGTT ACTCAGATTT AGAAGGGGAT TTCGATGTTG
     1441 CTGTTTTGCC ATTTTCCAAC AGCACAAATA ACGGGTTATT GTTTATAAAT ACTACTATTG
     1501 CCAGCATTGC TGCTAAAGAA GAAGGGGTAT CTCTCGAGAA AAGAGAGGCT GAAGCTTACG
     1561 TAGAATTCTT GGACAACGGA TTGGCTAGAA CTCCAACTAT GGGTTGGTTG CACTGGGAAA
     1621 GATTCATGTG TAACTTGGAC TGTCAAGAGG AACCAGACTC CTGTATTTCC GAGAAGTTGT
     1681 TCATGGAAAT GGCTGAGTTG ATGGTTTCTG AGGGATGGAA GGATGCTGGT TACGAGTACT
     1741 TGTGTATCGA CGACTGTTGG ATGGCTCCAC AAAGAGACTC TGAGGGAAGA TTGCAAGCTG
     1801 ACCCACAAAG ATTCCCACAC GGTATCAGAC AATTGGCTAA CTACGTTCAC TCCAAGGGAT
     1861 TGAAGTTGGG AATCTACGCT GATGTTGGTA ACAAGACTTG TGCTGGTTTC CCTGGTTCTT
     1921 TCGGTTACTA CGACATCGAC GCTCAAACTT TTGCTGATTG GGGTGTTGAC TTGTTGAAGT
     1981 TCGACGGTTG TTACTGTGAC TCCTTGGAGA ACTTGGCTGA TGGTTACAAG CACATGTCCT
     2041 TGGCTTTGAA CAGAACTGGT AGATCCATCG TTTACTCTTG TGAGTGGCCA TTGTACATGT
     2101 GGCCATTCCA GAAGCCAAAC TACACTGAGA TCAGACAGTA CTGTAACCAC TGGAGAAACT
     2161 TCGCAGACAT TGACGACTCT TGGAAGTCCA TTAAGTCCAT CTTGGACTGG ACATCCTTCA
     2221 ACCAAGAGAG AATCGTTGAC GTTGCTGGTC CTGGTGGATG GAACGATCCA GACATGTTGG
     2281 TTATCGGTAA CTTCGGTTTG TCCTGGAACC AACAGGTTAC ACAGATGGCT TTGTGGGCTA
     2341 TTATGGCTGC TCCATTGTTC ATGTCCAACG ACTTGAGACA CATTTCCCCA CAAGCTAAGG
     2401 CTTTGTTGCA GGACAAGGAC GTTATTGCTA TCAACCAGGA CCCATTGGGT AAACAAGGTT
     2461 ACCAGTTGAG ACAGGGTGAC AACTTCGAAG TTTGGGAAAG ACCATTGTCT GGATTGGCTT
     2521 GGGCTGTTGC TATGATCAAC AGACAAGAGA TCGGTGGTCC AAGATCCTAC ACTATCGCTG
     2581 TTGCTTCATT GGGTAAAGGT GTTGCTTGTA ACCCAGCTTG TTTCATCACT CAGTTGTTGC
     2641 CAGTTAAGAG AAAGTTGGGA TTCTATGAGT GGACTTCCAG ATTGAGATCC CACATCAACC
     2701 CAACTGGTAC TGTTTTGTTG CAGTTGGAGA ACACTATGCA GATGTCCTTG AAGGACTTGT
     2761 TGGAGCAGAA GTTGATTTCC GAAGAGGACT TGAACTCTGC TGTTGATCAT CATCACCATC
     2821 ACCACTAACC TTAATTAATG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC
     2881 GGAATTCAGC GCCAAAAGCT CTTTTATCTC AACCACTACT ACTGATTTAA CAAGTATAAA
     2941 CACTAGTGCG TATTCCACTG GATCCATTTC CACAGTAGAA ACAGGCAATC GAACTACATC
     3001 AGAAGTGATC AGCCATGTGG TGACTACCAG CACAAAACTG TCTCCAACTG CTACTACCAG
     3061 CCTGACAATT GCACAAACCA GTATCTATTC TACTGACTCA ACTATCACAG TAGGAACAGA
     3121 TATTCACACC ACATCAGAAG TGATTAGTGA TGTGGAAACC ATTAGCAGAG AAACAGCTTC
     3181 GACCGTTGTA GCCGCTCCAA CCTCAACAAC TGGATGGACA GGCGCTATGA ATACTTACAT
     3241 CTCGCAATTT ACATCCTCTT CTTTCGCAAC AATCAACAGC ACACCAATAA TCTCTTCATC
     3301 AGCAGTATTT GAAACCTCAG ATGCTTCAAT TGTCAATGTG CACACTGAAA ATATCACGAA
     3361 TACTGCTGCT GTTCCATCTG AAGAGCCCAC TTTTGTAAAT GCCACGAGAA ACTCCTTAAA
     3421 TTCCTTCTGC AGCAGCAAAC AGCCATCCAG TCCCTCATCT TATACGTCTT CCCCACTCGT
     3481 ATCGTCCCTC TCCGTAAGCA AAACATTACT AAGCACCAGT TTTACGCCTT CTGTGCCAAC
     3541 ATCTAATACA TATATCAAAA CGAAAAATAC GGGTTACTTT GAGCACACGG CTTTGACAAC
     3601 ATCTTCAGTT GGCCTTAATT CTTTTAGTGA AACAGCAGTC TCATCTCAGG GAACGAAAAT
     3661 TGACACCTTT TTAGTGTCAT CCTTGATCGC ATATCCTTCT TCTGCATCAG GAAGCCAATT
     3721 GTCCGGTATC CAACAGAATT TCACATCAAC TTCTCTCATG ATTTCAACCT ATGAAGGTAA
     3781 AGCGTCTATA TTTTTCTCAG CTGAGCTCGG TTCGATCATT TTTCTGCTTT TGTCGTACCT
     3841 GCTATTCTAA GCGGCCGCGA ATTAATTCGC CTTAGACATG ACTGTTCCTC AGTTCAAGTT
     3901 GGGCACTTAC GAGAAGACCG GTCTTGCTAG ATTCTAATCA AGAGGATGTC AGAATGCCAT
     3961 TTGCCTGAGA GATGCAGGCT TCATTTTTGA TACTTTTTTA TTTGTAACCT ATATAGTATA
     4021 GGATTTTTTT TGTCATTTTG TTTCTTCTCG TACGAGCTTG CTCCTGATCA GCCTATCTCG
     4081 CAGCTGATGA ATATCTTGTG GTAGGGGTTT GGGAAAATCA TTCGAGTTTG ATGTTTTTCT
     4141 TGGTATTTCC CACTCCTCTT CAGAGTACAG AAGATTAAGT GAGACCTTCG TTTGTGCGGA
     4201 TCCCCCACAC ACCATAGCTT CAAAATGTTT CTACTCCTTT TTTACTCTTC CAGATTTTCT
     4261 CGGACTCCGC GCATCGCCGT ACCACTTCAA AACACCCAAG CACAGCATAC TAAATTTCCC
     4321 CTCTTTCTTC CTCTAGGGTG TCGTTAATTA CCCGTACTAA AGGTTTGGAA AAGAAAAAAG
     4381 AGACCGCCTC GTTTCTTTTT CTTCGTCGAA AAAGGCAATA AAAATTTTTA TCACGTTTCT
     4441 TTTTCTTGAA AATTTTTTTT TTTGATTTTT TTCTCTTTCG ATGACCTCCC ATTGATATTT
     4501 AAGTTAATAA ACGGTCTTCA ATTTCTCAAG TTTCAGTTTC ATTTTTCTTG TTCTATTACA
     4561 ACTTTTTTTA CTTCTTGCTC ATTAGAAAGA AAGCATAGCA ATCTAATCTA AGGGCGGTGT
     4621 TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA
     4681 AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC
     4741 GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC
     4801 CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC
     4861 GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC
     4921 GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA
     4981 GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT
     5041 GGCCGAGGAG CAGGACTGAC ACGTCCGACG CGGCCCGACG GGTCCGAGGC CTCGGAGATC
     5101 CGTCCCCCTT TTCCTTTGTC GATATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC
     5161 CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC
     5221 CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC
     5281 TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA
     5341 AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCAAGCTGGA GACCAACATG TGAGCAAAAG
     5401 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     5461 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     5521 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     5581 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     5641 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     5701 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     5761 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     5821 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     5881 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     5941 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     6001 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     6061 GGTCTGACGC TCAGTGGA
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