ID pPSDzeo-FLAG-stuffer-V5-SAG1; SV 1; circular; unknown; STD; UNC; 6078 BP. DT 14-MRT.-2022 DE LMBP 9523. OS unknown CC Accession number: LMBP 9523 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 14-MAR-2022 FH Key Location/Qualifiers FH FT promoter 44..809 FT /label="AOX1 promoter" FT misc_feature 810..974 FT /label="5' UTR of AOX1" FT CDS 986..1228 FT /label="ppMF" FT misc_feature 1253..1276 FT /label="FLAG epitope" FT CDS 1290..1532 FT /label="ppMF" FT CDS 1569..2762 FT /label="hGLA" FT misc_feature 2763..2792 FT /label="Myc epitope" FT misc_feature 2808..2825 FT /label="His-tag" FT misc_feature 2840..2881 FT /label="V5 epitope" FT CDS 2888..3850 FT /label="SAG1 fragment" FT terminator 3938..4197 FT /label="AOX1 terminator" FT promoter 4204..4611 FT /label="TEF1 promoter" FT promoter 4619..4684 FT /label="synthetic EM7 promoter" FT CDS 4685..5059 FT /label="zeocin resistance" FT terminator 5105..5375 FT /label="CYC1 terminator" FT rep_origin complement(5385..5665) FT /label="pMB1 ori" SQ SEQUENCE 6078 AA; 1874484 MW; ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATCAGA TCTAACATCC AAAGACGAAA 60 GGTTGAATGA AACCTTTTTG CCATCCGACA TCCACAGGTC CATTCTCACA CATAAGTGCC 120 AAACGCAACA GGAGGGGATA CACTAGCAGC AGACCGTTGC AAACGCAGGA CCTCCACTCC 180 TCTTCTCCTC AACACCCACT TTTGCCATCG AAAAACCAGC CCAGTTATTG GGCTTGATTG 240 GAGCTCGCTC ATTCCAATTC CTTCTATTAG GCTACTAACA CCATGACTTT ATTAGCCTGT 300 CTATCCTGGC CCCCCTGGCG AGGTTCATGT TTGTTTATTT CCGAATGCAA CAAGCTCCGC 360 ATTACACCCG AACATCACTC CAGATGAGGG CTTTCTGAGT GTGGGGTCAA ATAGTTTCAT 420 GTTCCCCAAA TGGCCCAAAA CTGACAGTTT AAACGCTGTC TTGGAACCTA ATATGACAAA 480 AGCGTGATCT CATCCAAGAT GAACTAAGTT TGGTTCGTTG AAATGCTAAC GGCCAGTTGG 540 TCAAAAAGAA ACTTCCAAAA GTCGGCATAC CGTTTGTCTT GTTTGGTATT GATTGACGAA 600 TGCTCAAAAA TAATCTCATT AATGCTTAGC GCAGTCTCTC TATCGCTTCT GAACCCCGGT 660 GCACCTGTGC CGAAACGCAA ATGGGGAAAC ACCCGCTTTT TGGATGATTA TGCATTGTCT 720 CCACATTGTA TGCTTCCAAG ATTCTGGTGG GAATACTGCT GATAGCCTAA CGTTCATGAT 780 CAAAATTTAA CTGTTCTAAC CCCTACTTGA CAGCAATATA TAAACAGAAG GAAGCTGCCC 840 TGTCTTAAAC CTTTTTTTTT ATCATCATTA TTAGCTTACT TTCATAATTG CGACTGGTTC 900 CAATTGACAA GCTTTTGATT TTAACGACTT TTAACGACAA CTTGAGAAGA TCAAAAAACA 960 ACTAATTATT CGAAGGATCC AAACGATGAG ATTTCCTTCA ATTTTTACTG CAGTTTTATT 1020 CGCAGCATCC TCCGCATTAG CTGCTCCAGT CAACACTACA ACAGAAGATG AAACGGCACA 1080 AATTCCGGCT GAAGCTGTCA TCGGTTACTC AGATTTAGAA GGGGATTTCG ATGTTGCTGT 1140 TTTGCCATTT TCCAACAGCA CAAATAACGG GTTATTGTTT ATAAATACTA CTATTGCCAG 1200 CATTGCTGCT AAAGAAGAAG GGGTATCTCT CGAGAAAAGA GAGGCCGAAG CGGATTACAA 1260 AGACGATGAC GATAAGGCCG AGATGGCCGA TGAGATTTCC TTCAATTTTT ACTGCAGTTT 1320 TATTCGCAGC ATCCTCCGCA TTAGCTGCTC CAGTCAACAC TACAACAGAA GATGAAACGG 1380 CACAAATTCC GGCTGAAGCT GTCATCGGTT ACTCAGATTT AGAAGGGGAT TTCGATGTTG 1440 CTGTTTTGCC ATTTTCCAAC AGCACAAATA ACGGGTTATT GTTTATAAAT ACTACTATTG 1500 CCAGCATTGC TGCTAAAGAA GAAGGGGTAT CTCTCGAGAA AAGAGAGGCT GAAGCTTACG 1560 TAGAATTCTT GGACAACGGA TTGGCTAGAA CTCCAACTAT GGGTTGGTTG CACTGGGAAA 1620 GATTCATGTG TAACTTGGAC TGTCAAGAGG AACCAGACTC CTGTATTTCC GAGAAGTTGT 1680 TCATGGAAAT GGCTGAGTTG ATGGTTTCTG AGGGATGGAA GGATGCTGGT TACGAGTACT 1740 TGTGTATCGA CGACTGTTGG ATGGCTCCAC AAAGAGACTC TGAGGGAAGA TTGCAAGCTG 1800 ACCCACAAAG ATTCCCACAC GGTATCAGAC AATTGGCTAA CTACGTTCAC TCCAAGGGAT 1860 TGAAGTTGGG AATCTACGCT GATGTTGGTA ACAAGACTTG TGCTGGTTTC CCTGGTTCTT 1920 TCGGTTACTA CGACATCGAC GCTCAAACTT TTGCTGATTG GGGTGTTGAC TTGTTGAAGT 1980 TCGACGGTTG TTACTGTGAC TCCTTGGAGA ACTTGGCTGA TGGTTACAAG CACATGTCCT 2040 TGGCTTTGAA CAGAACTGGT AGATCCATCG TTTACTCTTG TGAGTGGCCA TTGTACATGT 2100 GGCCATTCCA GAAGCCAAAC TACACTGAGA TCAGACAGTA CTGTAACCAC TGGAGAAACT 2160 TCGCAGACAT TGACGACTCT TGGAAGTCCA TTAAGTCCAT CTTGGACTGG ACATCCTTCA 2220 ACCAAGAGAG AATCGTTGAC GTTGCTGGTC CTGGTGGATG GAACGATCCA GACATGTTGG 2280 TTATCGGTAA CTTCGGTTTG TCCTGGAACC AACAGGTTAC ACAGATGGCT TTGTGGGCTA 2340 TTATGGCTGC TCCATTGTTC ATGTCCAACG ACTTGAGACA CATTTCCCCA CAAGCTAAGG 2400 CTTTGTTGCA GGACAAGGAC GTTATTGCTA TCAACCAGGA CCCATTGGGT AAACAAGGTT 2460 ACCAGTTGAG ACAGGGTGAC AACTTCGAAG TTTGGGAAAG ACCATTGTCT GGATTGGCTT 2520 GGGCTGTTGC TATGATCAAC AGACAAGAGA TCGGTGGTCC AAGATCCTAC ACTATCGCTG 2580 TTGCTTCATT GGGTAAAGGT GTTGCTTGTA ACCCAGCTTG TTTCATCACT CAGTTGTTGC 2640 CAGTTAAGAG AAAGTTGGGA TTCTATGAGT GGACTTCCAG ATTGAGATCC CACATCAACC 2700 CAACTGGTAC TGTTTTGTTG CAGTTGGAGA ACACTATGCA GATGTCCTTG AAGGACTTGT 2760 TGGAGCAGAA GTTGATTTCC GAAGAGGACT TGAACTCTGC TGTTGATCAT CATCACCATC 2820 ACCACTAACC TTAATTAATG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 2880 GGAATTCAGC GCCAAAAGCT CTTTTATCTC AACCACTACT ACTGATTTAA CAAGTATAAA 2940 CACTAGTGCG TATTCCACTG GATCCATTTC CACAGTAGAA ACAGGCAATC GAACTACATC 3000 AGAAGTGATC AGCCATGTGG TGACTACCAG CACAAAACTG TCTCCAACTG CTACTACCAG 3060 CCTGACAATT GCACAAACCA GTATCTATTC TACTGACTCA ACTATCACAG TAGGAACAGA 3120 TATTCACACC ACATCAGAAG TGATTAGTGA TGTGGAAACC ATTAGCAGAG AAACAGCTTC 3180 GACCGTTGTA GCCGCTCCAA CCTCAACAAC TGGATGGACA GGCGCTATGA ATACTTACAT 3240 CTCGCAATTT ACATCCTCTT CTTTCGCAAC AATCAACAGC ACACCAATAA TCTCTTCATC 3300 AGCAGTATTT GAAACCTCAG ATGCTTCAAT TGTCAATGTG CACACTGAAA ATATCACGAA 3360 TACTGCTGCT GTTCCATCTG AAGAGCCCAC TTTTGTAAAT GCCACGAGAA ACTCCTTAAA 3420 TTCCTTCTGC AGCAGCAAAC AGCCATCCAG TCCCTCATCT TATACGTCTT CCCCACTCGT 3480 ATCGTCCCTC TCCGTAAGCA AAACATTACT AAGCACCAGT TTTACGCCTT CTGTGCCAAC 3540 ATCTAATACA TATATCAAAA CGAAAAATAC GGGTTACTTT GAGCACACGG CTTTGACAAC 3600 ATCTTCAGTT GGCCTTAATT CTTTTAGTGA AACAGCAGTC TCATCTCAGG GAACGAAAAT 3660 TGACACCTTT TTAGTGTCAT CCTTGATCGC ATATCCTTCT TCTGCATCAG GAAGCCAATT 3720 GTCCGGTATC CAACAGAATT TCACATCAAC TTCTCTCATG ATTTCAACCT ATGAAGGTAA 3780 AGCGTCTATA TTTTTCTCAG CTGAGCTCGG TTCGATCATT TTTCTGCTTT TGTCGTACCT 3840 GCTATTCTAA GCGGCCGCGA ATTAATTCGC CTTAGACATG ACTGTTCCTC AGTTCAAGTT 3900 GGGCACTTAC GAGAAGACCG GTCTTGCTAG ATTCTAATCA AGAGGATGTC AGAATGCCAT 3960 TTGCCTGAGA GATGCAGGCT TCATTTTTGA TACTTTTTTA TTTGTAACCT ATATAGTATA 4020 GGATTTTTTT TGTCATTTTG TTTCTTCTCG TACGAGCTTG CTCCTGATCA GCCTATCTCG 4080 CAGCTGATGA ATATCTTGTG GTAGGGGTTT GGGAAAATCA TTCGAGTTTG ATGTTTTTCT 4140 TGGTATTTCC CACTCCTCTT CAGAGTACAG AAGATTAAGT GAGACCTTCG TTTGTGCGGA 4200 TCCCCCACAC ACCATAGCTT CAAAATGTTT CTACTCCTTT TTTACTCTTC CAGATTTTCT 4260 CGGACTCCGC GCATCGCCGT ACCACTTCAA AACACCCAAG CACAGCATAC TAAATTTCCC 4320 CTCTTTCTTC CTCTAGGGTG TCGTTAATTA CCCGTACTAA AGGTTTGGAA AAGAAAAAAG 4380 AGACCGCCTC GTTTCTTTTT CTTCGTCGAA AAAGGCAATA AAAATTTTTA TCACGTTTCT 4440 TTTTCTTGAA AATTTTTTTT TTTGATTTTT TTCTCTTTCG ATGACCTCCC ATTGATATTT 4500 AAGTTAATAA ACGGTCTTCA ATTTCTCAAG TTTCAGTTTC ATTTTTCTTG TTCTATTACA 4560 ACTTTTTTTA CTTCTTGCTC ATTAGAAAGA AAGCATAGCA ATCTAATCTA AGGGCGGTGT 4620 TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA 4680 AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC 4740 GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC 4800 CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC 4860 GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC 4920 GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA 4980 GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT 5040 GGCCGAGGAG CAGGACTGAC ACGTCCGACG CGGCCCGACG GGTCCGAGGC CTCGGAGATC 5100 CGTCCCCCTT TTCCTTTGTC GATATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC 5160 CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC 5220 CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC 5280 TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA 5340 AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCAAGCTGGA GACCAACATG TGAGCAAAAG 5400 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 5460 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 5520 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 5580 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 5640 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 5700 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 5760 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 5820 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 5880 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 5940 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 6000 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 6060 GGTCTGACGC TCAGTGGA 6078 //