LOCUS       pMET7-FLAG-hFTH1        3607 bp    DNA     circular      5-FEB-2016
DEFINITION  LMBP 9528.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9528
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            
            
COMMENT     VNTDATE|-11094586|
COMMENT     VNTNAME|pMET7-FLAG-hFTH1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMET7-FLAG-hFTH1" 0 3607 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3567 3566 1 3607 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "SRalpha promoter" 29 0 0 9 632 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "9..632") 
COMMENT        #1=(CFSignal (CObList) "SV40 ori" 33 0 0 247 322 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "247..322") 
COMMENT        #2=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1490 1586 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5948..6044") 
COMMENT        #3=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1490 1586 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5948..6044)") 
COMMENT        #4=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2615 3475 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7073..7933)") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 1672 2074 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6130..6532)") 
COMMENT        #6=(CFSignal (CObList) "hFTH1" 4 0 0 865 1413 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "FLAG epitope" 22 0 0 811 834 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA Plasmid 'pMET7-FLAG-hFTH1'" 1) 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9528." 
COMMENT                         1) 
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COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                    #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Created: 02/05/16 12:01" 1) 
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COMMENT                         "Last Modified: 02/05/16 12:01" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3607 bp" 1) 
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COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 31-OCT-2012" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                "FLAG epitope"))) "Misc. Marker (1 total)") 
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COMMENT                         "Promoter Eukaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        9..632
                     /vntifkey="29"
                     /label=SRalpha\promoter
                     /note="SV40 early/human T-cell lymphotropic virus LTR hybrid promoter"
     rep_origin      247..322
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1490..1586
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1490..1586)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(2615..3475)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(1672..2074)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             865..1413
                     /vntifkey="4"
                     /label=hFTH1
     misc_marker     811..834
                     /vntifkey="22"
                     /label=FLAG\epitope
BASE COUNT      908 a       940 c       847 g       912 t 
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat 
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact 
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg gtgcaaatca 
      721 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc 
      781 tgctctaaaa gctgcgggcc cgccaccatg gattacaagg atgacgacga taaggaattg 
      841 acaagtttgt acaaaaaagc tggcatgacg accgcgtcca cctcgcaggt gcgccagaac 
      901 taccaccagg actcagaggc cgccatcaac cgccagatca acctggagct ctacgcctcc 
      961 tacgtttacc tgtccatgtc ttactacttt gaccgcgatg atgtggcttt gaagaacttt 
     1021 gccaaatact ttcttcacca atctcatgag gagagggaac atgctgagaa actgatgaag 
     1081 ctgcagaacc aacgaggtgg ccgaatcttc cttcaggata tcaagaaacc agactgtgat 
     1141 gactgggaga gcgggctgaa tgcaatggag tgtgcattac atttggaaaa aaatgtgaat 
     1201 cagtcactac tggaactgca caaactggcc actgacaaaa atgaccccca tttgtgtgac 
     1261 ttcattgaga cacattacct gaatgagcag gtgaaagcca tcaaagaatt gggtgaccac 
     1321 gtgaccaact tgcgcaagat gggagcgccc gaatctggct tggcggaata tctctttgac 
     1381 aagcacaccc tgggagacag tgataatgaa agctgcccaa ctttcttgta caaagtggtt 
     1441 tgatctagag aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 
     1501 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1621 tcaatgtatc ttatcatgtc tggatccccg ggtaccgagc tcgaattaat tgctcttccg 
     1681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 
     1741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 
     1801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 
     1861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 
     1921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 
     1981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 
     2041 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 
     2101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 
     2161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 
     2221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 
     2281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 
     2341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 
     2401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 
     2461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 
     2521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 
     2581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 
     2641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 
     2701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 
     2761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 
     2821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 
     2881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 
     2941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 
     3001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 
     3061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 
     3121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 
     3181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 
     3241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 
     3301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 
     3361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 
     3421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 
     3481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     3541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     3601 cacctgc 
//
