LOCUS       pGADT7-hPTPN6           9951 bp    DNA     circular      8-FEB-2016
DEFINITION  LMBP 9548.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTDATE|-11098829|
COMMENT     VNTNAME|pGADT7-hPTPN6|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGADT7-hPTPN6" 0 9951 
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COMMENT       (CMolPar 0 0 0 0 4294967295 1 9951 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "386..487") 
COMMENT        #2=(CFSignal (CObList) "LEU2" 4 0 1 690 1784 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(690..1784)") 
COMMENT        #3=(CFSignal (CObList) "GALad" 4 0 0 7453 7860 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "T7 promoter" 30 0 0 7866 7882 0 (CStringList) 
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COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 2601 3003 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3544 4404 0 
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COMMENT        #7=(CFSignal (CObList) "loxP" 21 0 1 2512 2545 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "loxP" 21 0 1 2401 2401 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ADH1 promoter" 29 0 0 7038 7442 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "hPTPN6" 4 0 0 8030 9817 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pGADT7-hPTPN6'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 02/08/16 10:44" 1) 
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COMMENT                          "Last Modified: 02/08/16 10:44" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9951 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 5-FEB-2016" 1))) 
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FEATURES             Location/Qualifiers
     CDS             7903..7929
                     /vntifkey="4"
                     /label=HA\epitope
     terminator      386..487
                     /vntifkey="43"
                     /label=ADH1\terminator
     CDS             complement(690..1784)
                     /vntifkey="4"
                     /label=LEU2
     CDS             7453..7860
                     /vntifkey="4"
                     /label=GALad
     promoter        7866..7882
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(2601..3003)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3544..4404)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    complement(2512..2545)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(2401..2401)
                     /vntifkey="21"
                     /label=loxP
     promoter        7038..7442
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             8030..9817
                     /vntifkey="4"
                     /label=hPTPN6
BASE COUNT     2776 a      2287 c      2286 g      2602 t 
ORIGIN
        1 tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat 
       61 cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata 
      121 ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact 
      181 agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc 
      241 ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt 
      301 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag 
      361 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa 
      421 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg 
      481 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca 
      541 tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa 
      601 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg 
      661 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg 
      721 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct 
      781 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat 
      841 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt 
      901 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct 
      961 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct 
     1021 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt 
     1081 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta 
     1141 ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa 
     1201 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa 
     1261 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg 
     1321 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg 
     1381 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga 
     1441 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt 
     1501 tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca 
     1561 cctaacaaaa cggcatcaac cttcttggag gcttccagcg cctcatctgg aagtgggaca 
     1621 cctgtagcgt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg 
     1681 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca 
     1741 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct 
     1801 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga 
     1861 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta 
     1921 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg 
     1981 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg 
     2041 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc 
     2101 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc 
     2161 atcttacgat acctgagtat tcccacagtt ggggatctcg actctagcta gaggatcaat 
     2221 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     2281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     2341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     2401 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca 
     2461 gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg 
     2521 tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag 
     2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     2701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     2761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     2821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     2881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     2941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     3001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     3061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     3121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     3181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     3241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     3301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     3721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     3781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     3841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     3901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     3961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     4021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     4081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     4141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     4201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     4261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     4321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4501 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     4561 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 
     4621 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     4681 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     4741 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa 
     4801 gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat 
     4861 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg 
     4921 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag 
     4981 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa 
     5041 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa 
     5101 cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc 
     5161 catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta 
     5221 tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg 
     5281 aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa 
     5341 accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa 
     5401 gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg 
     5461 cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg 
     5521 aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa 
     5581 gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt 
     5641 tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc 
     5701 tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat 
     5761 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca 
     5821 gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct 
     5881 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag 
     5941 cacagatgct tcgttgctcg gtgcatgcct gcaggtcgag atccgggatc gaagaaatga 
     6001 tggtaaatga aataggaaat caaggagcat gaaggcaaaa gacaaatata agggtcgaac 
     6061 gaaaaataaa gtgaaaagtg ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga 
     6121 gtttacgcaa ttgcacaatc atgctgactc tgtggcggac ccgcgctctt gccggcccgg 
     6181 cgataacgct gggcgtgagg ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg 
     6241 tttaccctgc gctaaggggc gagattggag aagcaataag aatgccggtt ggggttgcga 
     6301 tgatgacgac cacgacaact ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt 
     6361 tgcaacatga gtatactaga agaatgagcc aagacttgcg agacgcgagt ttgccggtgg 
     6421 tgcgaacaat agagcgacca tgaccttgaa ggtgagacgc gcataaccgc tagagtactt 
     6481 tgaagaggaa acagcaatag ggttgctacc agtataaata gacaggtaca tacaacactg 
     6541 gaaatggttg tctgtttgag tacgctttca attcatttgg gtgtgcactt tattatgtta 
     6601 caatatggaa gggaacttta cacttctcct atgcacatat attaattaaa gtccaatgct 
     6661 agtagagaag gggggtaaca cccctccgcg ctcttttccg atttttttct aaaccgtgga 
     6721 atatttcgga tatccttttg ttgtttccgg gtgtacaata tggacttcct cttttctggc 
     6781 aaccaaaccc atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt 
     6841 ggcggagggg agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga 
     6901 ctacaccaat tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta 
     6961 gacttgatag ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg 
     7021 aagtaataat aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg 
     7081 ttgtctcacc atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta 
     7141 acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatctcgaag 
     7201 cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag caacggtata 
     7261 cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa 
     7321 gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct cgttcccttt 
     7381 cttccttgtt tctttttctg cacaatattt caagctatac caagcataca atcaactcca 
     7441 agctttgcaa agatggataa agcggaatta attcccgagc ctccaaaaaa gaagagaaag 
     7501 gtcgaattgg gtaccgccgc caattttaat caaagtggga atattgctga tagctcattg 
     7561 tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca aacaaattct 
     7621 caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa 
     7681 atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc tggttggacg 
     7741 gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg 
     7801 gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc aaaaaaagag 
     7861 atctttaata cgactcacta tagggcgagc gccgccatgg agtacccata cgacgtacca 
     7921 gattacgctc atatggccat ggaggccagt gaattcaaag gcatggagag tagaaaactg 
     7981 atttctgcta cagacattca gtactctggc agtctgctga actccttgaa tggtgaggtg 
     8041 gtttcaccga gacctcagtg ggctggatgc agagaccctg ctcaagggcc gaggtgtcca 
     8101 cggtagcttc ctggctcggc ccagtcgcaa gaaccagggt gacttctcgc tctccgtcag 
     8161 ggtgggggat caggtgaccc atattcggat ccagaactca ggggatttct atgacctgta 
     8221 tggaggggag aagtttgcga ctctgacaga gctggtggag tactacactc agcagcaggg 
     8281 tgtcctgcag gaccgcgacg gcaccatcat ccacctcaag tacccgctga actgctccga 
     8341 tcccactagt gagaggtggt accatggcca catgtctggc gggcaggcag agacgctgct 
     8401 gcaggccaag ggcgagccct ggacgtttct tgtgcgtgag agcctcagcc agcctggaga 
     8461 cttcgtgctt tctgtgctca gtgaccagcc caaggctggc ccaggctccc cgctcagggt 
     8521 cacccacatc aaggtcatgt gcgagggtgg acgctacaca gtgggtggtt tggagacctt 
     8581 cgacagcctc acggacctgg tggagcattt caagaagacg gggattgagg aggcctcagg 
     8641 cgcctttgtc tacctgcggc agccgtacta tgccacgagg gtgaatgcgg ctgacattga 
     8701 gaaccgagtg ttggaactga acaagaagca ggagtccgag gatacagcca aggctggctt 
     8761 ctgggaggag tttgagagtt tgcagaagca ggaggtgaag aacttgcacc agcgtctgga 
     8821 agggcagcgg ccagagaaca agggcaagaa ccgctacaag aacattctcc cctttgacca 
     8881 cagccgagtg atcctgcagg gacgggacag taacatcccc gggtccgact acatcaatgc 
     8941 caactacatc aagaaccagc tgctaggccc tgatgagaac gctaagacct acatcgccag 
     9001 ccagggctgt ctggaggcca cggtcaatga cttctggcag atggcgtggc aggagaacag 
     9061 ccgtgtcatc gtcatgacca cccgagaggt ggagaaaggc cggaacaaat gcgtcccata 
     9121 ctggcccgag gtgggcatgc agcgtgctta tgggccctac tctgtgacca actgcgggga 
     9181 gcatgacaca accgaataca aactccgtac cttacaggtc tccccgctgg acaatggaga 
     9241 cctgattcgg gagatctggc attaccagta cctgagctgg cccgaccatg gggtccccag 
     9301 tgagcctggg ggtgtcctca gcttcctgga ccagatcaac cagcggcagg aaagtctgcc 
     9361 tcacgcaggg cccatcatcg tgcactgcag cgccggcatc ggccgcacag gcaccatcat 
     9421 tgtcatcgac atgctcatgg agaacatctc caccaagggc ctggactgtg acattgacat 
     9481 ccagaagacc atccagatgg tgcgggcgca gcgctcgggc atggtgcaga cggaggcgca 
     9541 gtacaagttc atctacgtgg ccatcgccca gttcattgaa accactaaga agaagctgga 
     9601 ggtcctgcag tcgcagaagg gccaggagtc ggagtacggg aacatcacct atcccccagc 
     9661 catgaagaat gcccatgcca aggcctcccg cacctcgtcc aaacacaagg aggatgtgta 
     9721 tgagaacctg cacactaaga acaagaggga ggagaaagtg aagaagcagc ggtcagcaga 
     9781 caaggagaag agcaagggtt ccctcaagag gaagtgaact cctttgaaat actagaaagt 
     9841 tttgttttgg atgatggggc aggggtttca gaagatctgt aaaacaaatt aaggtgcgaa 
     9901 caagttaatt tgatctgcca cattatctga aggaagtgta gtgggatttt c 
//
