LOCUS       pGADT7-hFOXK1           9876 bp    DNA     circular      8-FEB-2016
DEFINITION  LMBP 9552.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTDATE|-11098878|
COMMENT     VNTNAME|pGADT7-hFOXK1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGADT7-hFOXK1" 0 9876 
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COMMENT        (CObList) -1 "") 
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 9876 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "7903..7929") 
COMMENT        #1=(CFSignal (CObList) "ADH1 terminator" 43 0 0 386 487 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "386..487") 
COMMENT        #2=(CFSignal (CObList) "LEU2" 4 0 1 690 1784 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(690..1784)") 
COMMENT        #3=(CFSignal (CObList) "GALad" 4 0 0 7453 7860 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7453..7860") 
COMMENT        #4=(CFSignal (CObList) "T7 promoter" 30 0 0 7866 7882 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "7866..7882") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 2601 3003 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3544 4404 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3544..4404)") 
COMMENT        #7=(CFSignal (CObList) "loxP" 21 0 1 2512 2545 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "loxP" 21 0 1 2401 2401 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ADH1 promoter" 29 0 0 7038 7442 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "hFOXK1" 4 0 0 8030 9742 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pGADT7-hFOXK1'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 9552." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 02/08/16 11:33" 1) 
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COMMENT                          "Last Modified: 02/08/16 11:33" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9876 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 5-FEB-2016" 1))) 
COMMENT                   "Standard Fields") 
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FEATURES             Location/Qualifiers
     CDS             7903..7929
                     /vntifkey="4"
                     /label=HA\epitope
     terminator      386..487
                     /vntifkey="43"
                     /label=ADH1\terminator
     CDS             complement(690..1784)
                     /vntifkey="4"
                     /label=LEU2
     CDS             7453..7860
                     /vntifkey="4"
                     /label=GALad
     promoter        7866..7882
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(2601..3003)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3544..4404)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    complement(2512..2545)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(2401..2401)
                     /vntifkey="21"
                     /label=loxP
     promoter        7038..7442
                     /vntifkey="29"
                     /label=ADH1\promoter
     CDS             8030..9742
                     /vntifkey="4"
                     /label=hFOXK1
BASE COUNT     2658 a      2435 c      2208 g      2575 t 
ORIGIN
        1 tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat 
       61 cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata 
      121 ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact 
      181 agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc 
      241 ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt 
      301 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag 
      361 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa 
      421 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg 
      481 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca 
      541 tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa 
      601 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg 
      661 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg 
      721 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct 
      781 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat 
      841 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt 
      901 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct 
      961 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct 
     1021 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt 
     1081 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta 
     1141 ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa 
     1201 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa 
     1261 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg 
     1321 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg 
     1381 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga 
     1441 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt 
     1501 tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca 
     1561 cctaacaaaa cggcatcaac cttcttggag gcttccagcg cctcatctgg aagtgggaca 
     1621 cctgtagcgt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg 
     1681 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca 
     1741 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct 
     1801 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga 
     1861 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta 
     1921 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg 
     1981 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg 
     2041 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc 
     2101 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc 
     2161 atcttacgat acctgagtat tcccacagtt ggggatctcg actctagcta gaggatcaat 
     2221 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     2281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     2341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     2401 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca 
     2461 gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg 
     2521 tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag 
     2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     2701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     2761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     2821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     2881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     2941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     3001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     3061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     3121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     3181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     3241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     3301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3421 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3481 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3541 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3601 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3661 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     3721 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     3781 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     3841 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     3901 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     3961 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     4021 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     4081 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     4141 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     4201 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     4261 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     4321 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4381 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4441 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4501 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     4561 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 
     4621 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     4681 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     4741 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa 
     4801 gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat 
     4861 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg 
     4921 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag 
     4981 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa 
     5041 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa 
     5101 cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc 
     5161 catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta 
     5221 tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg 
     5281 aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa 
     5341 accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa 
     5401 gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg 
     5461 cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg 
     5521 aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa 
     5581 gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt 
     5641 tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc 
     5701 tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat 
     5761 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca 
     5821 gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct 
     5881 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag 
     5941 cacagatgct tcgttgctcg gtgcatgcct gcaggtcgag atccgggatc gaagaaatga 
     6001 tggtaaatga aataggaaat caaggagcat gaaggcaaaa gacaaatata agggtcgaac 
     6061 gaaaaataaa gtgaaaagtg ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga 
     6121 gtttacgcaa ttgcacaatc atgctgactc tgtggcggac ccgcgctctt gccggcccgg 
     6181 cgataacgct gggcgtgagg ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg 
     6241 tttaccctgc gctaaggggc gagattggag aagcaataag aatgccggtt ggggttgcga 
     6301 tgatgacgac cacgacaact ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt 
     6361 tgcaacatga gtatactaga agaatgagcc aagacttgcg agacgcgagt ttgccggtgg 
     6421 tgcgaacaat agagcgacca tgaccttgaa ggtgagacgc gcataaccgc tagagtactt 
     6481 tgaagaggaa acagcaatag ggttgctacc agtataaata gacaggtaca tacaacactg 
     6541 gaaatggttg tctgtttgag tacgctttca attcatttgg gtgtgcactt tattatgtta 
     6601 caatatggaa gggaacttta cacttctcct atgcacatat attaattaaa gtccaatgct 
     6661 agtagagaag gggggtaaca cccctccgcg ctcttttccg atttttttct aaaccgtgga 
     6721 atatttcgga tatccttttg ttgtttccgg gtgtacaata tggacttcct cttttctggc 
     6781 aaccaaaccc atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt 
     6841 ggcggagggg agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga 
     6901 ctacaccaat tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta 
     6961 gacttgatag ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg 
     7021 aagtaataat aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg 
     7081 ttgtctcacc atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta 
     7141 acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatctcgaag 
     7201 cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag caacggtata 
     7261 cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa 
     7321 gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct cgttcccttt 
     7381 cttccttgtt tctttttctg cacaatattt caagctatac caagcataca atcaactcca 
     7441 agctttgcaa agatggataa agcggaatta attcccgagc ctccaaaaaa gaagagaaag 
     7501 gtcgaattgg gtaccgccgc caattttaat caaagtggga atattgctga tagctcattg 
     7561 tccttcactt tcactaacag tagcaacggt ccgaacctca taacaactca aacaaattct 
     7621 caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa 
     7681 atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc tggttggacg 
     7741 gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg 
     7801 gatgatgtat ataactatct attcgatgat gaagataccc caccaaaccc aaaaaaagag 
     7861 atctttaata cgactcacta tagggcgagc gccgccatgg agtacccata cgacgtacca 
     7921 gattacgctc atatggccat ggaggccagt gaattcaaag gcatggagag tagaaaactg 
     7981 atttctgcta cagacattca gtactctggc agtctgctga actccttgaa tggcttactg 
     8041 ccttggagtt aatttcgttc cttctcgttt ctgctaccag cttcacaggc ttctgctaag 
     8101 gtgtaccttc cggtttccca gcacggccat caagatccag ttcacgtcgc tctatcacaa 
     8161 agaagaggcc ccagcctccc cgctgcggcc actgtacccc cagatctccc ctctgaagat 
     8221 ccacatcccg gagccggacc tccggagcat ggtcagcccc gtcccctccc cgacgggcac 
     8281 catcagtgtc cccaactcct gcccagccag tccacgcggt gccggctcct ccagttaccg 
     8341 ctttgtgcag aacgtgacct cggacctgca gctggcagca gagtttgcag caaaggccgc 
     8401 gtcggagcag caggcagaca cgtctggagg agacagcccc aaggatgagt caaagccgcc 
     8461 gttctcctac gcgcagctga tcgtgcaggc catctcctcc gcccaggacc ggcagctgac 
     8521 cctgagcggg atctacgccc acatcaccaa gcattacccc tactaccgga cggccgacaa 
     8581 aggctggcag aattctatcc ggcacaacct ctctttgaac cgttacttta tcaaagtccc 
     8641 acgttcccag gaggagcctg ggaaggggtc cttttggcga atagaccctg cctctgaagc 
     8701 caagctcgtg gaacaggcat tccggaaacg gaggcagagg ggtgtctcct gcttccgcac 
     8761 ccccttcggg cctctgtcct caaggagcgc tccagcttcg cccacacacc ccgggctgat 
     8821 gtcccctcgc tccggcggcc tgcagacccc agagtgcctg tctcgggagg gctcccccat 
     8881 tccacacgac cctgagtttg ggtccaagtt agcttctgtc ccagagtacc ggtattccca 
     8941 aagcgcaccc ggctcccccg tcagcgccca gccagtgatc atggccgtgc ctccccgacc 
     9001 gtccagcctc gtggccaagc ccgtggccta catgcccgcc tccatcgtaa cctcacagca 
     9061 gcccgcgggc cacgccatcc acgtcgtgca gcaggccccc accgtcacca tggtcagggt 
     9121 ggtcaccaca tctgccaact cggccaacgg atacatcctc accagccagg gcgcggcggg 
     9181 gggctcccat gatgcggcgg gcgcagccgt gctggacctg ggcagcgagg ccagaggcct 
     9241 ggaggagaaa cccaccattg cgtttgccac aatccccgcg gctggtggag tcatccagac 
     9301 ggtggccagc cagatggccc ccggggtccc cggacacacg gtcaccatcc tgcagcccgc 
     9361 cacacccgtg accctcgggc agcaccacct tccagtccgg gccgtgaccc agaacggaaa 
     9421 gcatgcggtt cccacgaaca gtttagccgg caacgcttac gccctcacca gccctttgca 
     9481 gctccttgcg acccaagcga gttcatccgc gccggtggtg gtcacccggg tgtgcgaggt 
     9541 ggggcccaag gagccagcag cagccgtcgc ggccacggcc accaccaccc cagccactgc 
     9601 caccaccgcc tctgcctccg cctcttccac tggagagccc gaggtcaaaa ggtcccgggt 
     9661 ggaggagccc agtggtgctg taaccacacc ggctggagtg atcgcagctg ccggccccca 
     9721 ggggccaggc accggggagt gaactccttt gaaatactag aaagttttgt tttggatgat 
     9781 ggggcagggg tttcagaaga tctgtaaaac aaattaaggt gcgaacaagt taatttgatc 
     9841 tgccacatta tctgaaggaa gtgtagtggg attttc 
//
