LOCUS       pDG1i-V5\blast          8383 bp    DNA     circular     23-JUN-2023
DEFINITION  LMBP 9591.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 9591
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-JUN-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     promoter        2252..2317
                     /label=synthetic\EM7\promoter
     CDS             268..927
                     /label=chloramhenicol\resistance
     CDS             1269..1574
                     /label=ccdB
     CDS             2318..2716
                     /label=blasticidin\resistance
     misc_recomb     1615..1739
                     /label=attR2
     misc_recomb     complement(35..159)
                     /label=attR1
     promoter        1866..2147
                     /label=SV40\early\promoter
     rep_origin      2104..2179
                     /label=SV40\ori
     terminator      3108..3182
                     /label=SV40\polyA\early
     promoter        6364..6692
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      3611..4066
                     /label=f1\ori
     CDS             4197..5057
                     /label=ampicillin\resistance
     rep_origin      5228..5819
                     /label=pMB1\ori
     promoter        6092..6175
                     /label=lac\promoter
     promoter        6239..6255
                     /label=T3\promoter
     terminator      complement(3108..3182)
                     /label=SV40\polyA\late
     rep_origin      3318..3393
                     /label=SV40\ori
     promoter        complement(3427..3443)
                     /label=T7\promoter
     misc_feature    6803..6847
                     /label=HIV-1\psi\packaging\signal
     misc_feature    7357..7590
                     /label=HIV-1\Rev\response\element
     terminator      2802..3036
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    2786..2801
                     /label=HIV-1\cPPT
     promoter        8107..8375
                     /label=hCMVTRE\promoter
     misc_feature    1792..1833
                     /label=V5\epitope
     promoter        167..219
                     /label=lacUV5\promoter
BASE COUNT     2260 a      1896 c      2089 g      2138 t 
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 
       61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 
      181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg 
      241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 
      301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 
      361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 
      421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 
      481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 
      541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 
      601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 
      661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 
      721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 
      781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 
      841 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 
      901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 
      961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 
     1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 
     1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 
     1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 
     1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg 
     1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 
     1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 
     1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 
     1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 
     1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 
     1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 
     1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 
     1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct 
     1801 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttggaat 
     1861 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 
     1921 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 
     1981 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 
     2041 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     2101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 
     2161 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 
     2221 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat 
     2281 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga 
     2341 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga 
     2401 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt 
     2461 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc 
     2521 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag 
     2581 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt 
     2641 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta 
     2701 tgtgtgggag ggctaagcac aattcgagct cggtaccttt aagaccaatg acttacaagg 
     2761 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 
     2821 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 
     2881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     2941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     3001 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 
     3061 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 
     3121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     3181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 
     3241 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat 
     3421 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac 
     3481 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 
     3541 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 
     3601 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     3661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     3721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     3781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     3841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     3901 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     3961 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     4021 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 
     4081 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
     4141 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 
     4201 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 
     4261 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 
     4321 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 
     4381 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 
     4441 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 
     4501 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 
     4561 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 
     4621 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 
     4681 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 
     4741 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 
     4801 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 
     4861 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 
     4921 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 
     4981 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 
     5041 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 
     5101 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     5161 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     5221 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     5281 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     5341 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     5401 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     5461 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     5521 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     5581 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     5641 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     5701 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     5761 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     5821 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     5881 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
     5941 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 
     6001 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
     6061 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 
     6121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 
     6181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa 
     6241 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat 
     6301 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa 
     6361 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag 
     6421 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat 
     6481 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc 
     6541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 
     6601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 
     6661 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg 
     6721 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca 
     6781 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag 
     6841 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa 
     6901 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca 
     6961 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt 
     7021 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca 
     7081 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc 
     7141 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa 
     7201 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt 
     7261 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag 
     7321 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt 
     7381 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca 
     7441 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca 
     7501 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt 
     7561 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat 
     7621 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg 
     7681 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca 
     7741 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt 
     7801 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa 
     7861 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc 
     7921 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac 
     7981 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga 
     8041 cagatccatt cgattagtga acggatctcg acggtatcga tggtccgagg ttctagacga 
     8101 gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga 
     8161 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat 
     8221 cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt 
     8281 ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta 
     8341 tataagcaga gctcgtttag tgaaccgtca gatcgcaccg gtc 
//
